Cystatins as calpain inhibitors: Engineered chicken cystatin- and stefin B-kininogen domain 2 hybrids support a cystatin-like mode of interaction with the catalytic subunit of μ-calpain

Biological Chemistry

Volumn 382, Issue 1, 2001, Pages 97-108

  Garcia Diaz B ^a   Gross, S ^a   Assfalg Machleidt, I ^a   Pfeiler, D ^a   Gollmitzer, N ^a   Gabrijelcic Geiger, D ^a   Stubbs, M T ^a   Fritz, H ^a   Auerswald, E A ^a   Machleidt, W ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Calpastatin; Kininogen; Papain; Stefin B; Surface plasmon resonance; Temporary inhibition

Indexed keywords

ALANINE; CALMODULIN; CALPAIN; CALPASTATIN; CYSTATIN; DIMER; GLYCINE; HYBRID PROTEIN; KININOGEN; MUTANT PROTEIN; PAPAIN; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; STEFIN B; SYNTHETIC PEPTIDE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CHEMICAL BOND; CHICKEN; CONTROLLED STUDY; DRUG STRUCTURE; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; GENE DELETION; GENE MUTATION; GENETIC ENGINEERING; HUMAN; INHIBITION KINETICS; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CALPAIN; CATALYSIS; CATALYTIC DOMAIN; CHICKENS; CLONING, MOLECULAR; CYSTATINS; CYSTEINE PROTEINASE INHIBITORS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTES; HUMANS; KININOGENS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

GALLUS GALLUS;

EID: 17744397102     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2001.015     Document Type: Article

References (41)

1. Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum
2. Hairpin loop mutations of chicken cystatin have different effects on the inhibition of cathepsin B, cathepsin L and papain
3. Hybrids of chicken cystatin with human kininogen domain 2 sequences exhibit novel inhibition of calpain, improved inhibition of actinidin and impaired inhibition of papain, cathepsin L and cathepsin B
4. Cathepsin B, Cathepsin H, and cathepsin L
5. Probing the functional role of the N-terminal region of cystatins by equilibrium and kinetic studies of the binding of Gly-11 variants of recombinant human cystatin C to target proteinases
6. 2+-induced conformational changes
7. The 2.0 Å X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases
8. Contiguous binding and inhibitory sites on kininogens required for the inhibition of platelet calpain
9. Calpain: A protease in search of a function?
10. The determination of protein, oligonucleotide and peptide molecular weights by ion-spray mass spectrometry
11. Inhibition of chicken calpain II by proteins of the cystatin superfamily and alpha 2-macroglobulin
12. Studies of the active site of m-calpain and the interaction with calpastatin
13. High-level expression of active human cystatin C in Escherichia coli
14. All four repeating domains of the endogenous inhibitor for calcium- dependent protease independently retain inhibitory activity. Expression of the cDNA fragments in Escherichia coli
15. Total synthesis of a gene for bovine rhodopsin
16. Quail cystatin: Isolation and characterisation of a new member of the cystatin family and its hypothetical interaction with cathepsin B
17. Structural basis for different inhibitory specificities of human cystatins C and D
18. Crystal structure of calpain reveals the structural basis for Ca(2+)-dependent protease activity and a novel mode of enzyme activation
19. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
20. Identification and characterization of inhibitory sequences in four repeating domains of the endogenous inhibitor for calcium-dependent protease
21. Total synthesis of a gene
22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
23. Crystal structure of calcium bound domain VI of calpain at 1.9 Å resolution and its role in enzyme assembly, regulation, and inhibitor binding
24. Amino-terminal conserved region in proteinase inhibitor domain of calpastatin potentiates its calpain inhibitory activity by interacting with calmodulin-like domain of the proteinase
25. Kinetics and molecular mechanism of inhibition of cysteine proteinases by their protein inhibitors
26. Temporary inhibition of papain by hairpin loop mutants of chicken cystatin. Distorted binding of the loops results in cleavage of the Gly(9)-Ala(10) bond
27. All four internally repetitive domains of pig calpastatin possess inhibitory activities against calpains I and II
28. Analysis of structure-function relationship of pig calpastatin by expression of mutated cDNAs in Escherichia coli
29. Inhibition of calpain by a synthetic oligopeptide corresponding to an exon of the human calpastatin gene
30. Interpreting mass spectra of multiply charged ions
31. Amino acid substitutions in the N-terminal segment of cystatin C create selective protein inhibitors of lysosomal cysteine proteinases
32. The slow-binding and slow, tight-binding inhibition of enzyme-catalysed reactions
33. r kininogen contains three copies of a cystatin sequence that are divergent in structure and in inhibitory activity for cysteine proteinases
34. The functional expression of antibody Fv fragments in Escherichia coli: Improved vectors and a generally applicable purification technique
35. Structure and physiological function of calpains
36. The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium
37. The refined 2.4 Å X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: A novel type of proteinase inhibitor interaction
38. A novel aspect of calpain activation
39. Preference of calcium-dependent interactions between calmodulin-like domains of calpain and calpastatin subdomains
40. The cystatins: Protein inhibitors of cysteine proteinases
41. Characterization of a functional domain of human calpastatin