Molecular beacons

Current Opinion in Chemical Biology

Volumn 8, Issue 5, 2004, Pages 547-553

  Tan, Weihong ^a,b   Wang, Kemim ^a,b   Drake, Timothy J ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b HUNAN UNIVERSITY   (China)

Author keywords

Fluorescence resonance energy transfer; FRET; MB; MBA; Molecular beacon; Molecular beacon aptamer; Resonance energy transfer; RET

Indexed keywords

DNA; DNA BINDING PROTEIN; DNA FRAGMENT; MESSENGER RNA; NUCLEIC ACID; PROTEIN; RNA;

ANISOTROPY; BIOSENSOR; CELL STRAIN COS7; COMPUTER PROGRAM; DNA CLEAVAGE; DNA HYBRIDIZATION; DNA PROBE; GENE EXPRESSION; GENETIC TRANSFECTION; GENOMICS; HUMAN; MOLECULAR RECOGNITION; NONHUMAN; ONCOGENE C FOS; POLYMERASE CHAIN REACTION; PROTEIN DNA INTERACTION; PROTEIN SYNTHESIS; PROTEOMICS; REVIEW; RNA ANALYSIS; RNA SEQUENCE; RNA SYNTHESIS; SIGNAL TRANSDUCTION;

BIOSENSING TECHNIQUES; DNA; DNA PROBES; EXTRACELLULAR SPACE; MICROSCOPY, FLUORESCENCE; MOLECULAR PROBE TECHNIQUES; PROTEINS; RNA; SENSITIVITY AND SPECIFICITY;

EID: 4644289739     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2004.08.010     Document Type: Review

References (40)

1. S. Tyagi, and F.R. Kramer Molecular beacons: probes that fluoresce upon hybridization Nat Biotechnol 14 1996 303 308
2. O. Noya, M.E. Patarroyo, F. Guzman, and B.A. De Noya Immunodiagnosis of parasitic diseases with synthetic peptides Curr Prot Peptide Sci 4 2003 299 308
3. X. Fang, J. Li, J. Perlette, K. Wang, and W. Tan Molecular beacons: novel fluorescent probes Anal Chem 72 2000 747A 753A
4. K. Wang, J. Li, X. Fang, S. Schuster, M. Vicens, S. Kelley, S. Lou, J.J. Li, T. Beck, R. Hogrefe, and W. Tan Novel fluorescent molecular beacon DNA probes for biomolecular recognition T. Vo-Dinh Biomedical Photonics Handbook 2003 CRC Press Washington 57.1 57.2
5. B. Solomon Towards diagnosis and treatment of Alzheimer's disease D.J. Selkoe Y. Christen Immunization Against Alzheimer's Disease and Other Neurodegenerative Disorders 2003 Springer-Verlag Heildelberg 11 19
6. A. Tsourkas, and G. Bao Shedding light on health and disease using molecular beacons Briefings in Funct Genomics Proteomics 1 2003 372 384
7. J.J. Li, R. Geyer, and W. Tan Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA Nucleic Acids Res 28 2003 e52
8. J. Perlette, J. Li, X. Fang, S. Schuster, J. Lou, and W. Tan Novel DNA probes for detection and quantification of protein molecules Rev Anal Chem 21 2002 1 14
9. S. Tyagi, D.P. Bratu, and F.R. Kramer Multicolor molecular beacons for allele discrimination Nat Biotechnol 16 1998 49 53
10. H. Wang, J. Li, H. Liu, Q. Liu, Q. Mei, Y. Wang, J. Zhu, N. He, and Z. Lu Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film Nucleic Acids Res 30 2002 e61
11. R. Manganelli, S. Tyagi, and I. Smith Real-time PCR using molecular beacons: a new tool to identify point mutations and to analyze gene expression in mycobacterium tuberculosis T. Parish N.G. Stoker Methods in Molecular Medicine vol. 54, Mycobacterium tuberculosis Protocols 2001 Humana Press 295 310
12. Vet JAM, Van der Rijt BJM, and H. Blom Molecular beacons: colorful analysis of nucleic acids Expert Rev Mol Diagn 2 2002 77 86
13. N.E. Broude Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology Trends Biotechnol 20 2002 249 256
14. H.J. Lou, and W. Tan Femtoliter microarray wells for ultrasensitive DNA/mRNA detection Instrumentation Sci Technol 30 2002 465 476
15. Marras SAE, F.R. Kramer, and S. Tyagi Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes Nucleic Acids Res 30 2002 e122
16. J.J. Li, and W. Tan A real-time assay for DNA sticky-end pairing using molecular beacons Anal Biochem 312 2003 251 254
17. S. Nozaki, Y. Tomioka, T. Hishinuma, M. Inoue, Y. Nagumo, L.R. Tsuruta, K. Hayashi, T. Matsumoto, Y. Kato, and S. Ishiwata Design, production, and characterization of recombinant neocarzinostatin apoprotein in Escherichia coli J Biochem 131 2002 729 738
18. Z. Tang, K. Wang, W. Tan, J. Li, L. Liu, Q. Guo, X. Meng, C. Ma, and S. Huang Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons Nucleic Acids Res 31 2003 e148
19. J.R. Epstein, Leung APK, K.H. Lee, and D.R. Walt High-density, microsphere-based fiber optic DNA microarrays Biosensors Bioelectronics 18 2003 541 546
20. A. Ramachandran, J. Flinchbaugh, P. Ayoubi, G.A. Olah, and J.R. Malayer Target discrimination by surface-immobilized molecular beacons designed to detect Francisella tularensis Biosensors Bioelectronics 19 2004 727 736
21. X. Zhao, R. Tapec-Dytioco, K. Wang, and W. Tan Collection of trace amounts of DNA/mRNA molecules using genomagnetic nanocapturers Anal Chem 75 2003 3476 3483
22. M.C. Daniel, and D. Astruc Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology Chem Rev 104 2004 293 346
23. H. Du, M.D. Disney, B.L. Miller, and T.D. Krauss Hybridization-based unquenching of DNA hairpins on au surfaces: prototypical "molecular beacon" biosensors J Am Chem Soc 125 2003 4012 4013
24. G. Yao, and W. Tan A molecular beacon based array for sensitive DNA analysis Anal Biochem 331 2004 216 223
25. G. Yao, X. Fang, H. Yokota, T. Yanagida, and W. Tan Monitoring molecular beacon DNA probe hybridization at single molecule level Chemistry 9 2003 5686 5692
26. T. Matsuo In situ visualization of messenger RNA for basic fibroblast growth factor in living cells Biochim Biophys Acta 1379 1998 178 184
27. A. Tsuji, H. Koshimoto, Y. Sato, M. Hirano, Y. Sei-Iida, S. Kondo, and K. Ishibashi Direct observation of specific messenger RNA in a single living cell under a fluorescence microscope Biophys J 78 2000 3260 3274
28. D.L. Sokol, X. Zhang, P. Lu, and A.M. Gewirtz Real time detection of DNA.RNA hybridization in living cells Proc Natl Acad Sci USA 95 1998 11538 11543
29. J. Perlette, and W. Tan Real time monitoring of mRNA hybridization in single living cells Anal Chem 73 2001 5544 5550
30. D.P. Bratu, B. Cha, M.M. Mhlanga, F.R. Kramer, and S. Tyagi Visualizing the distribution and transport of mRNAs in living cells Proc Natl Acad Sci USA 100 2003 13308 13313
31. P.J. Santangelo, B. Nix, A. Tsourkas, and G. Bao Dual FRET molecular beacons for mRNA detection in living cells Nucleic Acids Res 32 2004 e57
32. N. Nitin, P.J. Santangelo, G. Kim, S. Nie, and G. Bao Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells Nucleic Acids Res 32 2004 e58
33. J. Li, X. Fang, S. Schuster, and W. Tan Molecular beacons: a novel approach to detect protein-DNA interactions Angew Chem Int Ed 39 2000 1049 1052
34. J.J. Li, X. Fang, and W. Tan Molecular aptamer beacons for real-time protein recognition Biochem Biophys Res Comm 292 2002 31 40
35. A. Ellington, and J.W. Szostak In vitro selection of RNA molecules that bind specific ligands Nature 346 1990 818 822
36. D.L. Robertson, and G.F. Joyce Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA Nature 344 1990 467 468
37. C. Tuerk, and L. Gold Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase Science 249 1990 505 510
38. X. Fang, Z. Cao, T. Beck, and W. Tan Molecular aptamer for real-time oncoprotein platelet-derived growth factor monitoring by fluorescence anisotropy Anal Chem 73 2001 5752 5757
39. H.J. Lou, J.R. Brister, J.J. Li, W. Chen, N. Muzyczka, and W. Tan Adeno-associated virus Rep78/Rep68 promotes localized melting of the Rep binding element in the absence of ATP Chem Bio Chem 5 2004 100 108
40. X. Fang, A. Sen, M. Vicens, and W. Tan Synthetic DNA: aptamers to detect protein molecular variants in a high throughput fluorescence quenching assay Chem Bio Chem 4 2003 829 834