Sodium arsenite exposure alters cell migration, focal adhesion localization and decreases tyrosine phosphorylation of focal adhesion kinase in H9C2 myoblasts

Toxicological Sciences

Volumn 84, Issue 2, 2005, Pages 278-286

  Tancy, Shannon L ^a   Shelden, Eric A ^b   Gilmont, Robert R ^c   Welsh, Michael J ^c  

^a UNIVERSITY OF MICHIGAN SCHOOL OF PUBLIC HEALTH   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Cell migration; FAK; Focal adhesion; Paxillin; Phosphotyrosine; Sodium arsenite

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ACTIN; ARSENITE SODIUM; FOCAL ADHESION KINASE; INTEGRIN; NEUTRAL RED; PAXILLIN; PROPIDIUM IODIDE; TALIN; TRYPAN BLUE; TYROSINE; VINCULIN;

ACTIN POLYMERIZATION; ANIMAL CELL; APOPTOSIS; ARTICLE; AUTOPHOSPHORYLATION; CELL ADHESION; CELL MIGRATION; CELL PROLIFERATION; CELL VIABILITY; CELL VOLUME; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; ENVIRONMENTAL EXPOSURE; ENZYME INHIBITION; ENZYME LOCALIZATION; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; ENZYME STRUCTURE; HYPOTHESIS; METABOLISM; MYOBLAST; NONHUMAN; PROTEIN SYNTHESIS; RAT; SIGNAL TRANSDUCTION; STAINING;

ANIMALS; ARSENITES; BIOLOGICAL MARKERS; CELL ADHESION; CELL LINE, TRANSFORMED; CELL MOVEMENT; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; FOCAL ADHESION KINASE 1; FOCAL ADHESION PROTEIN-TYROSINE KINASES; MYOBLASTS; PHOSPHORYLATION; PROTEIN-TYROSINE KINASES; RATS; SODIUM COMPOUNDS; TYROSINE;

EID: 17444421557     PISSN: 10966080     EISSN: None     Source Type: Journal    
DOI: 10.1093/toxsci/kfi032     Document Type: Article

References (44)

1. Barchowsky, A., Roussel, R. R., Klei, L. R., James, P. E., Ganju, N., Smith, K. R., and Dudek, E. J. (1999). Low levels of arsenic trioxide stimulate proliferative signals in primary vascular cells without activating stress effector pathways. Toxicol. Appl. Pharmacol. 159, 65-75.
2. Bellis, S. L., Miller, J. T., and Turner, C. E. (1995). Characterization of the tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase. J. Biol. Chem. 270, 17437-17441.
3. Bode, A. M., and Dong, Z. (2002). The paradox of arsenic: Molecular mechanisms of cell transformation and chemotherapeutic effects. Crit. Rev. Oncol. Hematol. 42, 5-24.
4. Bonham, R. T., Fine, M. R., Pollock, F. M., and Shelden, E. A. (2003). Hsp27, Hsp70, and metallothionein in MDCK and LLC-PK1 renal epithelial cells: Effects of prolonged exposure to cadmium. Toxicol. Appl. Pharm. 191, 63-73.
5. Bonifacino, J. (1998). Metabolic labeling with amino acids. In Current Protocols in Molecular Biology (F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, and K. Struhl, Eds.), Section 10.18.1-10.18.10. John Wiley and Sons, New York, NY.
6. Burridge, K., and Chrzanowska-Wodnicka, M. (1996). Focal adhesions, contractility and signaling. Annu. Rev. Cell. Dev. Biol. 12, 463-519.
7. Chen, C. J., Chiou, H. Y., Chiang, M. H., Lin, L. J., and Tai, T. Y. (1996). Dose-response relationship between ischemic heart disease mortality and long-term arsenic exposure. Arterioscler. Thromb. Vasc. Biol. 16, 504-510.
8. Chou, I. N. (1989). Distinct cytoskeletal injuries induced by As, Cd, Co, Cr, and Ni compounds. Biomed. Environ. Sci. 2, 358-365.
9. Clark, E. A., and Brugge, J. S. (1995). Integrins and signal transduction pathway: The road taken. Science 268, 233-239.
10. Eide, B. L., Turck, C. W., and Escobedo, J. A. (1995). Identification of Tyr-397 as the primary site of tyrosine phosphorylation and pp60src association in the focal adhesion kinase, pp125FAK. Mol. Cell Biol. 15, 2819-2827.
11. Fincham, V. J., James, M., Frame, M. C., and Winder, S. J. (2000). Active ERK/ MAP kinase is targeted to newly forming cell-matrix adhesions by integrin engagement and v-Src. EMBO J. 19, 2911-2923.
12. Gebel, T. W. (2001). Unanswered questions in arsenic toxicity. J. Environ. Pathol. Toxicol. Oncol. 20, 299-309.
13. Harris, G. K., and Shi, X. (2003). Signaling by carcinogenic metals and metal-induced reactive oxygen species. Mutat. Res. 533, 183-200.
14. Hescheler, J., Meyer, R., Plant, S., Krautwurst, D., Rosenthal, W., and Schultz, G. (1991). Morphological, biochemical, and electrophysiological characterization of a clonal cell (H9C2) line from rat heart. Circ. Res. 69, 1476-1486.
15. Hossain, K., Akhand, A. A., Kato, M., Du, J., Takeda, K., Wu, J., Takeuchi, K., Liu, W., Suzuki, H., and Nakashima, I. (2000). Arsenite induces apoptosis of murine T lymphocytes through membrane raft-linked signaling for activation of c-Jun amino-terminal kinase. J. Immunol. 165, 4290-4297.
16. Hughes, M. F. (2002). Arsenic toxicity and potential mechanisms of action. Toxicol. Letters. 133, 1-16.
17. Illic, D., Damsky, C. H., and Yamamoto, T. (1997). Focal adhesion kinase at the cross roads of signal transduction. J. Cell Sci. 110, 401-407.
18. Ishrath, A., Kumar, N., and Dey, C. S. (2002). Differential activation of ERK and JNK by arsenite in mouse muscle cell. Comp. Biochem. Physiol. Part C 132, 375-384.
19. Keely, P., Parise, L., and Juliano, R. (1998). Integrins and GTPases in tumor cell growth, motility and invasion. Trends Cell. Biol 8, 101-106.
20. Lauffenburger, D. A., and Horwitz, A. F. (1996). Cell migration: A physically integrated molecular process. Cell 84, 359-369.
21. Li, W., and Chou, I. (1992). Effects of sodium arsenite on the cytoskeleton and cellular glutathione levels in cultured cells. Toxicol. Appl. Pharmacol. 114, 132-139.
22. Liu, J., Kadiiska, M., Liu, Y., Lu, T., Qu, W., and Waalkes, M. (2001). Stress related gene expression in mice treated with inorganic arsenicals. Toxicol. Sci. 61, 314-320.
23. Martin, K., Slack, J., Boerner, S., Matrin, C., and Parson, J. (2002). Integrins connections map: To affinity and beyond. Science 296, 1652-1653.
24. Morgan, D. M. (1998). Tetrazolium (MTT) assay for cellular viability and activity. Methods Mol. Biol. 79, 179-183.
25. Nickson, R., McArthur, J., Burgess, W., Ahmed, K. M., Ravencroft, P., and Rahman, M. (1998). Arsenic poisoning of Bangladesh groundwater. Nature 395, 338.
26. Norman, J. T., and Fine, L. G. (1999). Progressive renal disease: Fibroblasts, extracellular matrix, and integrins. Exp. Nephrol. 2, 167-177.
27. Obara, S., Nakata, M., Takeshima, H., Kuratsu, J., Maruyama, I., and Kitajima, I. (2002). Inhibition of migration of human glioblastoma cells by cerivastatin in association with focal adhesion kinase (FAK). Cancer Lett. 185, 153-161.
28. Porter, A. C., Fanger, G. R., and Vaillancourt, R. R. (1999). Signal transduction pathways regulated by arsenate and arsenite. Oncogene 18, 7794-7802.
29. Qian, Y., Castranova, V., and Shi, X. (2003). New perspectives in arsenic-induced cell signal transduction. J. Inorg. Biochem. 96, 271-278.
30. Ross, R. S., and Borg, T. K. (2001). Integrins and the myocardium. Circ. Res. 88, 1112-1119.
31. Sawada, K., Morishige, K., Tahara, M., Ikebuchi, Y., Kawagishi, R., Tasaka, K., and Murata, Y. (2002). Lysophophatidic acid induces focal adhesion assembly through Rho/Rho-associated kinase pathway in human ovarian cancer cells. Gynecol. Oncol. 87, 252-259.
32. Schaller, M. D., and Parsons, J. T. (1994). Focal adhesion kinase and associated proteins. Curr. Opin. Cell Biol. 6, 705-710.
33. Schaller, M. D., Hildebrand, J. D., Shannon, J. D., Fox, J. W., Vines, R. R., and Parsons, J. T. (1994). Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2dependent binding of pp60Src. Mol. Cell Biol. 14, 1680-1688.
34. Sharp, W. W., Simpson, D. G., Borg, T. K., Samarel, A. M., and Teffacio, L. (1997). Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes. Am. J. Physiol. 273, H546-H556.
35. Shi, H., Hudson, L. G., Ding, W., Wang, S., Cooper, K. L., Liu, S., Chen, Y., Shi, X., and Liu, K. J. (2004). Arsenite causes DNA damage in keratinocytes via generation of hydroxyl radicals. Chem. Res. Toxicol. 17, 871-878.
36. Singhal, P. C., Franki, N., Gibbons, N., and Hays, R. M. (1992). Effects of angiotensin II and arginine vasopressin on F-actin content of cultured mesangial cells. J. Am. Soc. Nephrol. 3, 80-87.
37. Trouba, K. J., Wauson, E. M., and Vorce, R. L. (2000). Sodium arsenite-induced dysregulation of proteins involved in proliferative signaling. Toxicol. Appl. Pharmacol. 164, 161-170.
38. Tseng, C. H., Tseng, C. P., Chiou, H. Y., Hsueh, Y. M., Chong, C. K., and Chen, C. J. (2002). Epidemiologic evidence of diabetogenic effect of arsenic. Toxicol. Lett. 133, 69-76.
39. Turner, C. E. (2000). Paxillin and focal adhesion signaling. Nat. Cell Biol. 2, E231-E236.
40. van de Water, B., Nagelkerke, J. F., and Stevens, J. L. (1999). Dephosphorylation of focal adhesion kinase (FAK) and loss of focal contacts precede caspase-mediated cleavage of FAK during apoptosis in renal epithelial cells. J. Biol. Chem. 274, 13328-13337.
41. van de Water, B., Houtepen, F., Huigsloot, M., and Tijdens, I. B. (2001). Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial cells (LLC-PK1) cells by focal adhesion kinase (FAK); role of FAK in maintaining focal adhesion organization after acute renal cell injury. J. Biol. Chem. 276, 36183-36193.
42. Wu, W., and Welsh, M. J. (1996). Expression of the 25-kDa heat-shock protein (HSP27) correlates with resistance to the toxicity of cadmium chloride, mercuric chloride, cis-platinum(II)-diammine dichloride, or sodium arsenite in mouse embryonic stem cells transfected with sense or antisense HSP27 cDNA. Toxicol. Appl. Pharmacol. 141, 330-339.
43. Yih, L., Peck, K., and Lee, T. (2002). Changes in gene expression profiles of human fibroblasts in response to sodium arsenite treatment. Carcinogenesis 23, 867-876.
44. Zamir, E., and Geiger, B. (2001). Components of cell-matrix adhesions. J. Cell Sci 114, 3577-3579.