In vitro adaptation of a ligase ribozyme for activity under a low-pH condition

Biotechnology and Bioengineering

Volumn 75, Issue 5, 2001, Pages 590-596

  Miyamoto, Yoshitaka ^a   Teramoto, Naozumi ^b   Imanishi, Yukio ^a   Ito, Yoshihiro ^c  

^a NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c UNIVERSITY OF TOKUSHIMA   (Japan)

Author keywords

Extremozyme; In vitro selection; Ligase; Ribozyme; SELEX

Indexed keywords

CLONING; MUTAGENESIS; PH EFFECTS; RNA;

CLONES;

ENZYMES;

LIGASE; RIBOZYME; RNA;

ACIDITY; ADAPTATION; ARTICLE; CHEMICAL REACTION; ENZYME ACTIVITY; IN VITRO STUDY; MOLECULAR CLONING; PH; REACTION ANALYSIS; RNA SEQUENCE; SEQUENCE ANALYSIS;

BASE SEQUENCE; CLONING, MOLECULAR; HYDROGEN-ION CONCENTRATION; LIGASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; NUCLEIC ACID CONFORMATION; OLIGONUCLEOTIDES; RNA; RNA, CATALYTIC; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 0035814390     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10033     Document Type: Article

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