Strain improvement and metabolic flux analysis in the wild-type and a mutant Lactobacillus lactis strain for L(+)-lactic acid production

Biotechnology and Bioengineering

Volumn 88, Issue 6, 2004, Pages 681-689

  Bai, Dong Mei ^a,b   Zhao, Xue Ming ^a   Li, Xin Gang ^a   Xu, Shi Min ^a  

^a TIANJIN UNIVERSITY   (China)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

L(+) lactic acid; Lactobacillus lactis; Metabolic flux analysis; NADH oxidase; Pyruvate; Strain improvement

Indexed keywords

BACTERIA; CELL CULTURE; ENZYMES; GLUCOSE; GROWTH KINETICS; METABOLISM; MUTAGENESIS;

CELL GROWTH; LACTIC ACID; METABOLIC FLUX ANALYSIS; PARENT STRAIN;

CARBOXYLIC ACIDS;

CALCIUM LACTATE; GLUCOSE; LACTIC ACID; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; MULTIENZYME COMPLEX; OXIDOREDUCTASE;

ARTICLE; BACTERIUM MUTANT; CONCENTRATION RESPONSE; ELIMINATION REACTION; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; LACTATE BLOOD LEVEL; LACTOBACILLUS DELBRUECKII; LACTOBACILLUS LACTIS BME5-18; MOLECULAR MECHANICS; MUTAGENESIS; NONHUMAN; PRODUCTIVITY; STRAIN IDENTIFICATION; ULTRAVIOLET RADIATION; WILD TYPE; BIOLOGICAL MODEL; BIOSYNTHESIS; COMPARATIVE STUDY; COMPUTER SIMULATION; ENERGY METABOLISM; GENE EXPRESSION REGULATION; GENETIC ENHANCEMENT; GENETICS; LACTOBACILLUS; METABOLISM; METHODOLOGY; PHYSIOLOGY; RADIATION EXPOSURE; SIGNAL TRANSDUCTION; SPECIES DIFFERENCE;

LACTOBACILLUS; LACTOBACILLUS DELBRUECKII SUBSP. LACTIS;

COMPUTER SIMULATION; ENERGY METABOLISM; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENHANCEMENT; GLUCOSE; LACTIC ACID; LACTOBACILLUS; MODELS, BIOLOGICAL; MULTIENZYME COMPLEXES; MUTAGENESIS; NADH, NADPH OXIDOREDUCTASES; SIGNAL TRANSDUCTION; SPECIES SPECIFICITY; ULTRAVIOLET RAYS;

EID: 16644383498     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20274     Document Type: Article

References (30)

1. Bai DM, Jia MZ, Zhao XM, Ban R, Shen F, Li XG, Xu XM. 2003. L(+)-lactic acid production by pellet form Rhizopus oryzae R1021 in a stirred tank fermenter. Chem Eng Sci 58:785-791.
2. Bai DM, Zhao XM, Li XG, Xu SM. 2004. Strain improvement of Rhizopus oryzae for over-production of L(+)-lactic acid and metabolic flux analysis of mutants. Biochem Eng J 18:41-48.
3. Banul J, Clifton D, Kretschmer M, Fraenkel DG. 1993. Glucose metabolism in Escherichia coli and the effect of increased amount of aldolase. Biochem 32:4685-4692.
4. Boelsa IC, van Kranenburga R, Hugenholtza J, Kleerebezema M, de Vosa WM. 2001. Sugar catabolism and its impact on the biosynthesis and engineering of exopolysaccharide production in lactic acid bacteria. International Dairy J 11:723-732.
5. Datta R, Tsai SP, Bonsignore P, Moon SH, Frank JR. 1995. The technologyical and economic potential of poly (lactic acid) and its derivatives. FEMS Microbiol Rev 16:221-231.
6. DeMan JC, Rogosa M, Sharpe ME. 1960. A medium for the cultivation of lactobacilli. J Appl Bact 23:130-135.
7. + ratio. J Bacteriol 179:5282-5287.
8. Hoefnagel MHN, Starrenburg MJ, Martens DE, Hugenholtz J, Kleerebezem M, van Swam II, Bongers R, Westerhoff HV, Snoep JL. 2002. Metabolic engineering of lactic acid bacteria, the combined approach: Kinetic modelling, metabolic control and experimental analysis. Microbiology 148:1003-1013.
9. Hols P, Kleerebezem M, Schank AN, Ferain T, Hugenholtz J, Delcour J, de Vos WM. 1999a. Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering. Nat Biotechnol 17:588-592.
10. Hols P, Ramos A, Hugenholtz J, Delcour J, de Vos WM, Santos H, Kleerebezem M. 1999b. Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: A rescue pathway for maintaining redox balance. J Bacteriol 181:5521-5526.
11. Hong SH, Lee SY. 2001. Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity. Biotechnol Bioeng 74:89-95.
12. Hua Q, Fu PC, Yang C, Shimizu K. 1998. Microaerobic lysine fermentation and metabolic flux analysis. Biochem Eng J 2:89-100.
13. Hugenholtz J, Hols P, Starrenburg MJC, de Vos WM, Kleerebezem M. 2000. Metabolic engineering of Lactococcus lactis leading to high diacetyl production. Appl Environ Microbiol 66:4112-4114.
14. Kleerebezem M, Boels IC, Groot MN, Mierau I, Sybesma W, Hugenholtz J. 2002. Metabolic engineering of Lactococcus lactis: The impact of genomics and metabolic modeling. J Biotechnol 98:199-213.
15. Longacre A, Reimers J, Gannon JE, Wright BE. 1997. Flux analysis of glucose metabolism in Rhzopus oryzae for the purpose of increasing lactate yields. J Theor Biol 21:30-39.
16. Lopez de Felipe F, Starrenburg MJC, Hugenholtz J. 1997. The role of NADH-oxidation in acetoin and diacetyl production from glucose in Lactococcus lactis. FEMS Microbiol Lett 156:15-19.
17. Lopez de Felipe F, Kleerebezem M, de Vos WM, Hugenholtz J. 1998. Cofactor engineering: A novel approach to metabolic engineering in Lactococcus lactis by controlled overexpression of NADH oxidase. J Bacteriol 180:3804-3808.
18. Nielsen J, Villadsen J, Gunnar L. 2003. Bioreaction engineering principles, 2nd ed. New York: Plenum Press.
19. Parekh S, Vinci VA, Strobel RJ. 2000. Improvement of microbial strains and fermentation processes. Appl Microbiol Biotechnol 54:287-301.
20. Siebold M, von Friling P, Joppien R, Rindfleisch D, Schügerl K, Röper H. 1995. Comparison of the production of lactic acid by three different lactobacilli and its recovery by extraction and electrodialysis. Proc Biochem 30:81-95.
21. Stephanopoulos GN, Aristidou AA, Nielsen J. 1998. Metabolic engineering. San Diego: Academic Press.
22. Stols L, Donnelly MI. 1997. Production of succinic acid through over-expression of NAD+-dependent malic enzyme in an Escherichia coli mutant. Appl Environ Microbiol 63:2695-2701.
23. Suntornsuk W, Hang YD. 1994. Strain improvement of Rhizopus oryzae for production of L(+)-lactic acid and glucoamylase. Lett Appl Mirobiol 19:249-252.
24. Tsai SP, Coleman RD, Moon SH, Schneider KA, Sanville Millard C. 1993. Strain screening and development for industrial lactic acid fermentation. Appl Biochem Biotechnol 39/40:323-335.
25. Tsai TS, Millard CS. 1994. Improved pretreatment process for lactic acid production. PCT Int Appl Patent, WO 94/13826; PCT/US93/11759.
26. Tyree RW, Clausen EC, Gaddy JL. 1990. The fermentative characteristics of Lactobacillus xylosus on glucose and xylose. Biotechnol Lett 12:51-56.
27. Vallino JJ, Stephanopoulos G. 1993. Metabolic flux distributions in Corynebaxterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41:633-646.
28. Venkatesh KV. 1997. Metabolic flux analysis of lactic acid fermentation: Effects of pH and lactate ion concentration. Proc Biochem 32(8):651-655.
29. Wright BE, Longacre A, Reimers J. 1996. Models of metabolism in Rhizopus oryzae. J Theor Biol 182:453-457.
30. Yang YT, Bennett GN, San KY. 2001. The effects of feed and intracellular pyruvate levels on the redistribution of metabolic fluxes in Escherichia coli. Metabol Eng 3:115-123.