The effects of feed and intracellular pyruvate levels on the redistribution of metabolic fluxes in Escherichia coli

Metabolic Engineering

Volumn 3, Issue 2, 2001, Pages 115-123

  Yang, Yea Tyng ^a   Bennett, George N ^a   San, Ka Yiu ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

Intracellular pyruvate; Lactate dehydrogenase; Metabolic engineering; Pyruvate feeding; Sensitivity coefficient

Indexed keywords

ACETYL COENZYME A; ALLOSTERISM; BACTERIAL METABOLISM; BACTERIUM MUTANT; BIOCHEMICAL PATHWAY; CATALYST ACTIVITY; ENZYME ACTIVATION; GENE OVEREXPRESSION; GENETIC STRAIN; LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVIC ACID; WILD RELATIVE;

ESCHERICHIA COLI;

EID: 0034816411     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.2000.0166     Document Type: Article

References (41)

1. Quantitative analysis of proton-linked transport systems
2. Intracellular concentration of coenzyme A and its derivatives from Clostridium acetobutylicum ATVV824 and their role in enzyme regulation
3. The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli
4. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid
5. The formation and metabolism of methylglycoxal during glycolysis in Escherichia coli
6. Assessment of a futile cycle involving reconversion of fructose 6-phosphate to fructose 1,6-phosphate during glyconeogenic growth of Escherichia coli
7. Bacterial lactate dehydrogenates
8. Physiological, biochemical, and mathematical studies of micro-aerobic continuous ethanol fermentation by Saccharomyces cerevisiae. II. Intracellular metabolite and enzyme assays at steady state chemostat cultures
9. Kinetics of growth and substrate consumption of Escherichia coli ML30 on two carbon sources
10. Phosphorylation/dephosphorylation of the receiver module at the conserved aspartate residue controls transphosphorylation activity of histidine kinase in sensor protein ArcB of Escherichia coli
11. Mutational analysis of signal transduction by ArcB: A membrane sensor protein for anaerobic expression of operons involved in the central aerobic pathways in Escherichia coli
12. Pyruvate formate-lyase is not essential for nitrate respiration by Escherichia coli
13. Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli
14. Ultrastructure and pyruvate formate-lyase radical quenching property of the multienzymatic adhE protein of Escherichia coli
15. Pyruvate formate-lyase reaction in Escherichia coli
16. Genetic control of the uptake of pyruvate by Escherichia coli
17. Characterization of the specific pyruvate transport system in Escherichia coli K-12
18. Role of protein phosphorylation in the regulation of aerobic metabolism by the Arc system in Escherichia coli
19. The effect of carbon and nitrogen sources on the level of metabolic intermediates in Escherichia coli
20. Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase
21. Effect of pyruvate on hexose metabolism by Escherichia coli
22. Intermediary metabolite levels in Escherichia coli
23. Growth yield and energy distribution
24. The nucleotide sequence of pACYC184
25. Specific transcriptional requirements for positive regulation of the anaerobically inducible pfl operon by ArcA and FNR
26. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12
27. Novel transcriptional control of the pyruvate formate-lyase gene: Upstream regulatory sequences and multiple promoters regulate anaerobic expression
28. Anaerobic induction of pyruvate formate-lyase gene expression is mediated by the ArcA and FNR proteins
29. 70-dependent transcription in vitro and heterologous expression in Pseudomonas putida in vivo
30. Responses of metabolic systems to large changes in enzyme activities and effectors. 1. The linear treatment for unbranched
31. Pyruvate catabolism during transient state conditions in chemostat cultures of Enterococcus faecalis NCTC 775: Importance of internal pyruvate concentration and NADH/NAD + ratios
32. Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coli
33. Periplasmic space in Sabnonella typhimurium and Escherichia coli
34. Chemical characterization of D-lactate dehydrogenase from Escherichia coli B
35. Kinetics of Escherichia coli B D-lactate dehydrogenase and evidence for pyruvate-controlled change in conformation
36. Metabolic uncoupling of substrate levelphosphorylation in anaerobic glucose-limited chemostat cultures of Klebsiellaaerogenes NCTC418
37. A pH-regulated promoter for the expression of recombinant proteins in Escherichia coli
38. From famine to feast - The role of methylglyoxal production in Escherichia coli
39. Metabolic flux analysis of E. coli deficient in the acetate production pathway and expressing the B. subtilis acetolactate synthase
40. Redistribution of metabolic fluxes in Escherichia coli with fermentative lactate dehydrogenase overexpression and deletion