The nuclear tRNA aminoacylation-dependent pathway may be the principal route used to export tRNA from the nucleus in Saccharomyses cerevisiae

Biochemical Journal

Volumn 378, Issue 3, 2004, Pages 809-816

  Steiner Mosonyi, Marta ^a   Mangroo, Dev ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Los1p; Nuclear tRNA aminoacylation; Nuclear tRNA export; Nucleolus; Protein translation; Utp8p

Indexed keywords

ACYLATION; AMINATION; CELLS; ENZYMES; QUALITY ASSURANCE; RNA;

AMINOACYLATION;

BIOCHEMISTRY;

TRANSFER RNA;

AMINOACYLATION; ARTICLE; CELL NUCLEUS; FLUORESCENCE IN SITU HYBRIDIZATION; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; RNA TRANSPORT; SACCHAROMYCES CEREVISIAE;

ACTIVE TRANSPORT, CELL NUCLEUS; CELL NUCLEOLUS; CELL NUCLEUS; MUTATION; NUCLEAR PORE COMPLEX PROTEINS; RNA, TRANSFER; RNA, TRANSFER, AMINO ACYL; RNA, TRANSFER, TYR; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION;

MAMMALIA; SACCHAROMYCES CEREVISIAE; SACCHAROMYSES CEREVISIAE;

EID: 1642464577     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031306     Document Type: Article

References (29)

1. Hopper, A. K. and Phizicky, E. M. (2003) tRNA transfers to the limelight. Genes Dev. 17, 162-180
2. Arts, G. J., Kuersten, S., Romby, P., Ehresmann, B. and Mattaj, I. W. (1998) The role of exportin-t in selective nuclear export of mature tRNAs. EMBO J. 17, 7430-7441
3. Lipowsky, G., Bischoff, F. R., Izaurralde, E., Kutay, U., Schafer, S., Gross, H. J., Beier, H. and Gorlich, D. (1999) Coordination of tRNA nuclear export with processing of tRNA. RNA 5, 539-549
4. Lund, E. and Dahlberg, J. E. (1998) Proofreading and aminoacylation of tRNAs before export from the nucleus. Science 282, 2082-2085
5. Sarkar, S. and Hopper, A. K. (1998) tRNA nuclear export in Saccharomyces cerevisiae: in situ hybridization analysis. Mol. Biol. Cell 9, 3041-3055
6. Sarkar, S., Azad, A. K. and Hopper, A. K. (1999) Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 96, 14366-14371
7. Sharma, K., Fabre, E., Tekotte, H., Hurt, E. C. and Tollervey, D. (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol. Cell Biol. 16, 294-301
8. Simos, G., Tekotte, H., Grosjean, H., Segref, A., Sharma, K., Tollervey, D. and Hurt, E. C. (1996) Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO J. 15, 2270-2284
9. Shen, W. C., Selvakumar, D., Stanford, D. R. and Hopper, A. K. (1993) The Saccharomyces cerevisiae LOS1 gene involved in pre-tRNA splicing encodes a nuclear protein that behaves as a component of the nuclear matrix. J. Biol. Chem. 268, 19436-19444
10. Yoshihisa, T., Yunoki-Esaki, K., Ohshima, C., Tanaka, N. and Endo, T. (2003) Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria. Mol. Biol. Cell 14, 3266-3279
11. Grosshans, H., Hurt, E. and Simos, G. (2000) An aminoacylation-dependent nuclear tRNA export pathway in yeast. Genes Dev. 14, 830-840
12. Azad, A. K., Stanford, D. R., Sarkar, S. and Hopper, A. K. (2001) Role of nuclear pools of aminoacyl-tRNA synthetases in tRNA nuclear export. Mol. Biol. Cell 12, 1381-1392
13. Steiner-Mosonyi, M., Leslie, D. M., Dehghani, H., Aitchison, J. D. and Mangroo, D. (2003) Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae. J. Biol. Chem. 278, 32236-32245
14. Arts, G., Fornerod, J. M. and Mattaj, I. W. (1998) Identification of a nuclear export receptor for tRNA. Curr. Biol. 8, 305-314.
15. Hellmuth, K. D., Lau, M., Bischoff, F. R., Kunzler, M., Hurt, E. and Simos, G. (1998) Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol. Cell Biol. 18, 6374-6386
16. Kutay, U., Lipowsky, G., Izaurralde, E., Bischoff, F. R., Schwarzmaier, P., Hartmann, E. and Gorlich, D. (1998) Identification of a tRNA-specific nuclear export receptor. Mol. Cell 1, 359-369
17. Calado, A., Treichel, N., Muller, E. C., Otto, A. and Kutay, U. (2002) Exportin-5-mediated nuclear export of eukaryotic elongation factor 1A and tRNA. EMBO J. 21, 6216-6224
18. Bohnsack, M. T., Regener, K., Schwappach, B., Saffrich, R., Paraskeva, E., Hartmann, E. and Gorlich, D. (2002) Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. EMBO J. 21, 6205-6215
19. Feng, W. and Hopper, A. K. (2002) A Los1p-independent pathway for nuclear export of intronless tRNAs in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 99, 5412-5417
20. Wise, J. A. (1991) Preparation and analysis of low molecular weight RNAs and small ribonucleoproteins. Methods Enzymol. 194, 405-415
21. Cleary, J. D. and Mangroo, D. (2000) Nucleotides of the tRNA D-stem that play an important role in nuclear-tRNA export in Saccharomyces cerevisiae. Biochem. J. 347, 115-122
22. Reference deleted.
23. Mangroo, D. and RajBhandary, U. L. (1995) Mutants of Escherichia coli initiator tRNA defective in initiation. Effects of overproduction of methionyl-tRNA transformylase and the initiation factors IF2 and IF3. J. Biol. Chem. 270, 12203-12209
24. Varshney, U., Lee, C. P. and RajBhandary, U. L. (1991) Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase. J. Biol. Chem. 266, 24712-24718
25. Ambrogelly, A., Ahel, I., Polycarpo, C., Bunjun-Srihari, S., Krett, B., Jacquin-Becker, C., Ruan, B., Kohrer, C., Stathopoulos, C., RajBhandary, U. L. and Soll, D. (2002) Methanocaldococcus jannaschii prolyl-tRNA synthetase charges tRNA(Pro) with cysteine. J. Biol. Chem. 277, 34749-34754
26. Bertrand, E., Houser-Scott, F., Kendall, A., Singer, R. H. and Engelke, D. R. (1998) Nucleolar localization of early tRNA processing. Genes Dev. 12, 2463-2468
27. Hurt, D. J., Wang, S. S., Lin, Y. H. and Hopper, A. K. (1987) Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing. Mol. Cell Biol. 7, 1208-1216
28. Ko, Y. G., Kang, Y. S., Kim, E. K., Park, S. G. and Kim, S. (2000) Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis. J. Cell Biol. 149, 567-574
29. Tschochner, H. and Hurt, E. (2003) Pre-ribosomes on the road from the nucleolus to the cytoplasm. Trends Cell Biol. 13, 255-263