Transient Bcl-2 gene down-expression in circulating mononuclear cells of severe sepsis patients who died despite appropriate intensive care

Intensive Care Medicine

Volumn 30, Issue 3, 2004, Pages 408-415

  Bilbault, Pascal ^a   Lavaux, Thomas ^b   Lahlou, Abdeslam ^b   Uring Lambert, Béatrice ^b   Gaub, Marie Pierre ^b   Ratomponirina, Charline ^b   Meyer, Nicolas ^b   Oudet, Pierre ^b   Schneider, Francis ^a  

^a HÔPITAL DE HAUTEPIERRE   (France)

^b UNIVERSITY HOSPITAL OF STRASBOURG   (France)

Author keywords

Apoptosis; Bcl 2 gene expression; Human; Lymphocyte subpopulations; Outcome; Severe sepsis

Indexed keywords

C REACTIVE PROTEIN; CD4 ANTIGEN; CD8 ANTIGEN; COMPLEMENTARY DNA; CYTOKINE; INTERLEUKIN 10; INTERLEUKIN 6; LIPOCORTIN 5; PROTEIN BAX; PROTEIN BCL 2; RNA; TUMOR NECROSIS FACTOR ALPHA;

ADULT; AGED; APOPTOSIS; ARTICLE; CHEMOLUMINESCENCE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; INTENSIVE CARE; LYMPHOCYTE COUNT; LYMPHOCYTE SUBPOPULATION; MALE; MONONUCLEAR CELL; REAL TIME POLYMERASE CHAIN REACTION; SEPSIS;

ANALYSIS OF VARIANCE; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CASE-CONTROL STUDIES; CYTOKINES; DOWN-REGULATION; FEMALE; GENE EXPRESSION; GENES, BCL-2; HUMANS; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE SUBSETS; MALE; MIDDLE AGED; PROSPECTIVE STUDIES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; STATISTICS, NONPARAMETRIC; SYSTEMIC INFLAMMATORY RESPONSE SYNDROME;

EID: 1642368260     PISSN: 03424642     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00134-003-2118-z     Document Type: Article

References (30)

1. Munford RS, Pugin J (2001) Normal responses to injury prevent systemic inflammation and can be immunosuppressive. Am J Respir Crit Care Med 163:316-321
2. Bone RC, Grodzin CJ, Balk RA (1997) Sepsis: a new hypothesis for pathogenesis of the disease process. Chest 112:235-243
3. Hotchkiss RS, Swanson PE, Freeman BD et al. (1999) Apoptotic cell death in patients with sepsis, shock, and multiple organ dysfunction. Crit Care Med 27:1230-1251
4. Docke WD, Randow F, Syrbe U et al. (1997) Monocyte deactivation in septic patient: restoration by IFNgamma treatment. Nat Med 3:678-681
5. Hotchkiss RS, Swanson PE, Cobb JP, Jacobson A, Buchman TH, Karl IE (1997) Apoptosis in lymphoid and parenchymal cells during sepsis: findings in normal and T and B cell-deficient mice. Crit Care Med 25:1298-1307
6. Hotchkiss RS, Tinsley KW, Swanson PE et al. (1999) Prevention of lymphocyte cell death in sepsis improves survival in mice. Proc Natl Acad Sci U S A 96:14541-14546
7. Hotchkiss RS, Swanson PE, Knudson CM et al. (1999) Over expression of Bcl-2 in transgenic mice decreases apoptosis and improves survival in sepsis. J Immunol 162:4148-4156
8. Coopersmith CM, Stanley PE, Davis CG, Arniot DR, Buchman TG, Karl IE, Hochkin RS (2002) Inhibition of intestinal epithelial apoptosis and survival in a murine model of pneumonia-induced sepsis. JAMA 287:1716-1721
9. Kroemer G (1997) The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nat Med 3:614-620
10. Cory S, Adams JM (2002) The Bcl-2 family: regeneration of the cellular life or death switch. Nat Rev Cancer 2:647-656
11. Korsmeyer SJ, Shutter JR, Veis DJ, Merry DE, Oltuai ZN (1993) Bcl-2/ Bax: a rheostat that regulates an antioxidant pathway and cell death. Cancer Biol 4:327-330
12. American College of Chest Physicians/ Society of Critical Care Medicine Consensus Conference (1992) Definition for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. Chest 101:1644-1655
13. Rivers E, Nguyen B, Haustad S et al. for the Early Goal-Directed Therapy Collaborative Group (2001) Early goal-direct therapy in the treatment of severe sepsis and septic shock. N Engl J Med 345:1368-1377
14. Le Gall JR, Lermeshow S, Saunier F (1993) A new simplified acute physiology score (SAPS II) based on European/North American multicenter study. JAMA 270:2957-2963
15. Le Gall JR, Klar J, Lemeshow S, Saunier F, Alberti C, Artigas A, Teres D for the ICU Scoring Group (1996) The logistic organ dysfunction system. A new way to assess organ dysfunction in the intensive care unit. JAMA 276:802-810
16. Vandesompece J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalisation of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:1-12
17. Cassinat B, Zassadowski F, Balitrand N et al. (2000) Quantitation of minimal residual disease in acute promyelotic leukemia patients with t(15;17) translocation using real-time RT-PCR. Leukemia 14:324-328
18. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta DeltaC(T)) method. Methods 25:402-408
19. Bone RC (1996) Immunologic dissonance: a continuing evolution in one understanding of the systemic inflammatory response syndrome and the multiple organ dysfunction syndrome. Ann Intern Med 125:680-687
20. Hotchkiss RS, Tinsley KW, Swanson PE et al. (2001) Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in human. J Immunol 166:6952-6963
21. Pinsky OR, Vincent JL, Device J, Alegre M, Kahn RJ, Dupont E (1993) Serum cytokine levels in human septic shock. Relation to multiple system organ failure and mortality. Chest 103:565-575
22. Presterl E, Staudinger T, Pettermann M et al. (1997) Cytokine profile and correlation to the Apache III and MPM II scores in patients with sepsis. Am J Respir Crit Care Med 156:825-832
23. Van Dissel JT, Van Langevelde P, Westendorps RG, Kwappenberg K, Frolich M (1998) Anti-inflammatory cytokine profile and mortality in febrile patients. Lancet 351:950-953
24. Adrie C, Bachelet M, Vayssier-Taussat M et al. (2001) Mitochondrial membrane potential and apoptosis peripheral blood monocytes in severe human sepsis. Am J Respir Crit Care Med 164:389-395
25. Oberholtzer C, Oberholtzer A, Clare-Salzler M, Moldawer LL (2001) Apoptosis in sepsis: a new target for therapeutic exploration. FASEB J 15:879-892
26. Nakayama K, Nakayama KI, Negiski I, Knida K, Santa H, Loh DY (1994) Targeted disruption of Bcl-2αβ in mice: occurrence of grey hair, polycystic kidney disease, and lymphocytopenia. Proc Nad Acad Sci U S A 91:3700-3704
27. Menges T, Engel J, Welters J et al. (1999) Changes in blood lymphocyte count after multiple trauma: association with post-traumatic complications. Crit Care Med 27:733-740
28. Le Tulzo Y, Pangault C, Gacoin A et al. (2002) Early circulating lymphocyte apoptosis in human septic shock is associated with poor outcome. Shock 18:487-494
29. Knudson CM, Tung KSK, Tourtelotte WG, Bronn AJ, Korsmeyer SJ (1995) Bax-deficient mice with lymphoid hyperplasia and male germ cell death. Science 270:96-99
30. Cheng T, Liu D, Griffin JH, Fernandez JA et al. (2003) Activated protein C blocks p53-mediated apoptosis in ischemic human brain endothelium and is neuroprotective. Nat Med 9:338-342