A novel neutral protease from Thermoactinomyces species 27a: Sequencing of the gene, purification, and characterization of the enzyme

Protein Journal

Volumn 23, Issue 7, 2004, Pages 483-492

  Zabolotskaya, Maria V ^a   Demidyuk, Ilya V ^a   Akimkina, Tatiana V ^a   Kostrov, Sergey V ^a  

^a INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

Author keywords

Enzymatic properties; Metalloproteinase; Sequence; Thermal stability; Thermoactinomyces

Indexed keywords

AMIDE; BENZYLSULFONYL FLUORIDE; EDETIC ACID; METALLOPROTEINASE; PHENANTHROLINE DERIVATIVE; POLYPEPTIDE; PROTEIN PRECURSOR; PROTEINASE; SIGNAL PEPTIDE; BACTERIAL PROTEIN; ENZYME PRECURSOR; PEPTIDE HYDROLASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACILLUS SUBTILIS; CALCULATION; CARBOXY TERMINAL SEQUENCE; CLONING; ELECTROPHORESIS; GENE SEQUENCE; HYDROLYSIS; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH MEASUREMENT; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; PURIFICATION; THERMOACTINOMYCES; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; MICROMONOSPORACEAE; MOLECULAR CLONING; MOLECULAR GENETICS;

BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME PRECURSORS; GENE EXPRESSION; MICROMONOSPORACEAE; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES;

EID: 16244384599     PISSN: 15723887     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10930-004-5225-y     Document Type: Article

References (55)

1. Abakov, A. S., Bolotin, A. P., and Sorokin, A. V. (1990). Mol. Biol. (Mosk) 24: 1363-1372.
2. Antonov, V. K. (1991). Khimiya proieoliza (Chermistry of Proteolysis). Nauka, Moscow.
3. Banbula, A., Potempa, J., Travis, J., Fernandez-Catalán, C., Mann, K., Huber, R., Bode, W., and Medrano, F. J. (1998). Structure 6: 1185-1193.
4. Barret, A. J., Rawlings, N. D., and Woessner, J. F. (1998). In: Handbook of Proteolytic Enzymes, Academic Press Inc., New York, pp. 1033-1064.
5. Bradford, M. M. (1976). Anal. Biochem. 72: 248-254.
6. Van den Burg, B., Enequist, H. G., Van der Haar, M. E., Eijsink, V. G. H., Stulp, B. K., and Venema, G. (1991). J. Bacteriol. 173: 4107-4115.
7. Charney, J. and Tomarelli, R. M. (1947). J. Biochem. 177: 501-505.
8. David, V. A., Deutch, A. H., Sloma, A., Pawlyk, D., Ally, A., and Durham, D. R. (1992). Gene 112: 107-112.
9. Demiduyk, I. V., Zabolotskaya, M. V., Velishayeva, N. S., Safina, D. R., and Kostrov, S. V. (2003). Mol. Gen. Mikrobiol. Vrusol. 4: 11-15.
10. Dhanaraj, V., Ye, Q. Z., Johnson, L. L., Hupe, D. J., Ortwine, D. F., Dunbar, J. B., Jr., Rubin, J. R., Pavlovsky, A., Humblet, C., and Blundell, T. L. (1996). Drug Des. Discov. 13: 3-14.
11. Feder, J. (1967). Biochemistry 6: 2088-2093.
12. Feder, J. (1968). Biochem. Biophys. Res. Commun. 32: 326-332.
13. Feder, J., and Schuck, J. M. (1970). Biochemistry 9: 2784-2791.
14. Feder, J., Garrett, L. R., and Wildi, B. S. (1971a). Biochemistry. 10: 4552-4556.
15. Feder, J., Keay, L., Garrett, L. R., Cirulis, N., Moseley, M. H., and Wildi, B. S (1971b). Biochim. Biophys. Acta 251: 74-78.
16. Gasparov, V. S., and Degtiar', V.G. (1994). Biokhimiia 59: 763-777.
17. Hase, C. C. and Finkelstein, R. A. (1991). J. Bacteriol. 173: 3311-3317.
18. Inouye, K. (1992). J. Biochem. (Tokyo) 112: 335-340.
19. Inouye, K., Lee, S. B., and Tonomura, B. (1996). Biochem. J. 315: 133-138.
20. Khan, S. M. and Darnall, D. W. (1978). Anal. Biochem. 86: 332-336. Au:Khan and Darnall (1978) not cited. Please cite reference in text or delete from the list.
21. Kuhn, S., and Fortnagel, P. (1993). J. Gen. Microbiol. 139: 39-47.
22. Laemmli, U. K. (1970). Nature 227: 680-685.
23. Latt, S. A., Holmquist, B., and Vallee, B. L. (1969). Biochem. Biophys. Res. Comm. 37: 333-339.
24. Lee, Y. C., Koike, H., Taguchi, H., Ohta, T., and Matsuzawa, H. (1994). FEMS Microbiol. Lett. 120: 69-74.
25. Matthews, B. W., Jansonius, J. N., Colman, P. M., Schoenborn, B. P., and Dupourque, D. (1972). Nature New Biol. 238: 37-41.
26. Miyoshi, S., Wakae, H., Tomochika, K., and Shinoda, S. (1997). J. Bacteriol. 179: 7606-7609.
27. Miyoshi, S., Sonoda, Y., Wakiyama, H., Rahman, M. M., Tomochika, K., Shinoda, S., Yamamoto, S., and Tobe, K. (2002). Microb. Pathog. 33: 127-134.
28. Morozova, I. P., Chestukhina, G. G., Bormatova, M. E., Gololobov, M. Yu., Ivanova, N. M., Lysogorskaya, E. N., Filippova, I. Yu., Khodova, O. M., Timokhina, E. A., and Stepanov, V. M. (1993). Biokhmtiia 58: 896-907.
29. Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G. (1997). Protein Eng. 10: 1-6.
30. Nishiya, Y. and Imanaka, T. (1990). J. Bacteriol. 172: 4861-4869.
31. Norqvist, A., Norrman, B., and Wolf-Watz, H. (1990). Infect. Immun. 58: 3731-3736.
32. O'Donohue, M. J., Roques, B. P., and Beaumont, A. (1994). Biochem. J. 300: 599-603.
33. Pauptit, R. A., Karlson, R., Picot, R., Jenkins, D., Niklaus-Reimer, A.-S., and Jansonius, J. N. (1988). J. Mol. Biol. 199: 525-538.
34. Pohlner, J., Halter, R., Beyreuther, K., and Meyer, T. F. (1987). Nature. 325: 458-462.
35. Pugsley, A. P. (1998). Protein Transfer and Organelle Biogenesis In: Das R. A. and Robins P. W. (eds), Academic Press. Orlando, FL, pp. 607-652.
36. Rawlings, N. D., Tolle, D. P., and Barrett, A. J. (2004). Nucleic Acids Res. Database issue. 32: D160-D164.
37. Sidler, W., Kumpf, B., Peterhaus, B., and Zuber, H. (1986). Appl. Microbiol. and Biotechnol. 25: 18-24.
38. Smith, H., de Jong, A., Bron, S., and Venema, G. (1988). Gene 70: 351-361.
39. Sohoni, S. S. and Joshi, P. N. (1982). J. Biochem. Biophys. (Indian) 19: 399-402.
40. Stark, W., Pauptit, R. A., Wilson, K. S., and Jansonius, J. N. (1992). Eur. J. Biochem. 207: 781-791.
41. Takagi, M., Imanaka, T., and Aiba, S. (1985). J. Bacteriol. 163: 824-831.
42. Takekawa, S., Uozumi, N., Tsukagoshi, N., and Udaka, S. (1991). J. Bacteriol. 173: 6820-6825.
43. Thayer, M. M., Flaherty, K. M., and McKay, D. B. (1991). J. Biol. Chem. 266: 2864-2871.
44. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., and Higgins, D. G. (1997). Nucleic Acids Research. 24: 4876-4882.
45. Titani, K., Hermodson, M. A., Ericsson, L. H., Walsh, K. A., and Neurath, H. (1972). Nature New Biol. 238: 35-37.
46. Tran, L., Wu, X. C., and Wong, S. L. (1991). J. Bacteriol. 173: 6364-6372.
47. Vasantha, N., Thompson, L. D., Rhodes, C., Banner, C., Nagie, J., and Filpula, D. (1984). J. Bacteriol. 159: 811-819.
48. Veltman, O. R., Vriend, G., van den Burg, B., Hardy, F., Venema, G., and Eijsink, V. G. (1997). FEBS Lett. 405: 241-244.
49. Watson, M. E. (1984). Nucleic Acids Res. 12: 5145-5164.
50. Weaver, L. H., Kester, W. R., and Matthews, B. W. (1977). J. Mol. Biol. 114: 119-132.
51. Wetmore, D. R., Wong, S. L., and Roche, R. S. (1992). Mol. Microbiol. 6: 1593-1604.
52. Wetmore, D. R., Wong, S. L., and Roche, R. S. (1994). Mol. Microbiol. 12: 747-759.
53. Yanagida, N., Uozumi, T., and Beppu, T. (1986). J. Bacteriol. 166: 937-944.
54. Yang, M. Y., Ferrari, E., and Henner, D. J. (1984). J. Bacteriol. 160: 15-21.
55. Zabolotskaya, M. V., Nosovskaya, E. A., Kaplun, M. A., and Akimkina, T. V. (2001). Mol. Gen. Mikrobiol. Virusol. 1: 32-34.