An exocellular thermolysin-like metalloprotease produced by Vibrio fluvialis: Purification, characterization, and gene cloning

Microbial Pathogenesis

Volumn 33, Issue 3, 2002, Pages 127-134

  Miyoshi, Shin Ichi ^a   Sonoda, Yuka ^a   Wakiyama, Hiroko ^a   Rahman, Md Monzur ^a   Tomochika, Ken Ichi ^a   Shinoda, Sumio ^a   Yamamoto, Shigeo ^a   Tobe, Kazuo ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Haemagglutinin; Metalloprotease; Protease; Thermolysin; Vibrio fluvialis

Indexed keywords

BACTERIAL ENZYME; CASEIN; CHELATING AGENT; ELASTIN; METALLOPROTEINASE; OLIGOPEPTIDE; THERMOLYSIN;

AMINO ACID SEQUENCE; ARTICLE; BLEEDING; CELL LEVEL; CISTRON; COMPETITIVE INHIBITION; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SYNTHESIS; HEMAGGLUTINATION; MEMBRANE PERMEABILITY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FAMILY; SEQUENCE ANALYSIS; STRUCTURAL GENE; VIBRIO FLUVIALIS; VIBRIO VULNIFICUS;

VIBRIO; VIBRIO FLUVIALIS; VIBRIO VULNIFICUS;

EID: 0036384304     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.2002.0520     Document Type: Article

References (18)

1. Hase CC, Finkelstein RA. Bacterial extracellular zinc-containing metallo-proteases. Micrbiol Rev 1993; 57: 823-37.
2. Miyoshi S, Shinoda S. Microbial metalloproteases and pathogenesis. Microbes Infect 2000; 2: 91-8.
3. Janda JM, Poweres C, Bryant RG, Abbott SL. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin Microbiol Rev 1988; 1: 245-67.
4. Miyoshi S, Oh EG, Hirata K, Shinoda S. Exocellular toxic factors produced by Vibrio vulnificus. J Toxicol Toxin Rev 1993; 12: 253-88.
5. Chowdhury MAR, Miyoshi S, Shinoda S. Puri(r)cation and characterization of a protease produced by Vibrio mimicus. Infect Immun 1990; 58: 4159-62.
6. Chowdhury MAR, Miyoshi S, Shinoda S. Role of Vibrio mimicus protease in enterotoxigenicity. J Diarroeal Dis Res 1991; 9: 332-4.
7. Chowdhury MAR, Miyoshi S, Shinoda S. Vascular permeability enhancement by Vibrio mimicus protease and the mechanisms of action. Microbiol Immunol 1991; 35: 1049-58.
8. Alam M, Miyoshi S, Shinoda, S. Production of antigenically related exocellular elastolytic proteases mediating haemagglutination by vibrios. Microbiol Immunol 1995; 39: 67-70.
9. Hase CC, Finkelstein RA. Cloning and nucleotide sequence of the Vibrio cholerae haemagglutinin/protease (HA/protease) gene and construction of an HA/ protease-negative strain. J Bacteriol 1991; 173: 3311-17.
10. Cheng JC, Shao CP, Hor LI. Cloning and nucleotide sequencing of the protease gene of Vibrio vulnificus. Gene 1996; 183: 255-7.
11. Miyoshi N, Shimizu C, Miyoshi S, Shinoda S. Purification and characterization of Vibrio vulnificus protease. Microbiol Immunol 1987; 31: 13-25.
12. Miyoshi S, Wakae H, Tomochika K, Shinoda S. Functional domains of a zinc metalloprotease from Vibrio vulnificus. J Bacteriol 1997; 179: 7606-9.
13. Morihara K, Tsuzuki H. Comparative study of various neutral proteinase from microorganisms: specificity with oligopeptides. Arch Biochem Biophys 1971; 146: 291-6.
14. Milton DL, Norqvist A, Wolf-Watz H. Cloning of a metalloprotease gene involved in the virulence mechanism of Vibrio anguillarum. J Bacteriol 1992; 174: 7235-44.
15. David VA, Deutch AH, Sloma A, Pawlyk D, Ally AH, Durham DR. Cloning, sequencing and expression of the gene encoding the extracellular neutral pro-tease, vibriolysin, of Vibrio proteolyticus. Gene 1992; 112: 107-12.
16. Kothary MH, Kreger AS. Production and partial characterization of an elastolytic protease of Vibrio vulnificus. Infect Immun 1985; 50: 534-40.
17. Davis BJ. Disc eletrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci 1964; 121: 404-27.
18. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.