Engineering thermolysin-like proteases whose stability is largely independent of calcium

FEBS Letters

Volumn 405, Issue 2, 1997, Pages 241-244

  Veltman, Oene R ^a   Vriend, Gert ^b   Van Den Burg, Bertus ^a   Hardy, Florence ^a,d   Venema, Gerard ^a   Eijsink, Vincent G H ^c  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c NLVF   (Norway)

^d UNIVERSITÉ DE CERGY PONTOISE   (France)

Author keywords

Autolysis; Calcium binding; Thermal stability; Thermolysin; Unfolding pathway

Indexed keywords

CALCIUM; PROTEINASE; THERMOLYSIN;

ARTICLE; AUTOLYSIS; CALCIUM BINDING; ENGINEERING; ENZYME STABILITY; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY;

BINDING SITES; CALCIUM; CALCIUM-BINDING PROTEINS; ENZYME STABILITY; HEAT; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; THERMOLYSIN;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0030992072     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(97)00193-2     Document Type: Article

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