Deletion of the NR2A subunit prevents developmental changes of NMDA-mEPSCs in cultured mouse cerebellar granule neurones

Journal of Physiology

Volumn 563, Issue 3, 2005, Pages 867-881

  Fu, Zhanyan ^a   Logan, Stephen M ^a   Vicini, Stefano ^a  

^a GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MAGNESIUM ION; N METHYL DEXTRO ASPARTIC ACID RECEPTOR 1; N METHYL DEXTRO ASPARTIC ACID RECEPTOR 2A; N METHYL DEXTRO ASPARTIC ACID RECEPTOR 2B; TRAXOPRODIL;

ANIMAL CELL; ARTICLE; CELL AGING; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DENDRITE; DEVELOPMENTAL STAGE; EXCITATORY POSTSYNAPTIC POTENTIAL; GENETIC TRANSFECTION; GRANULE CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; KNOCKOUT MOUSE; MOUSE; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NONHUMAN; PERIKARYON; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; QUALITATIVE ANALYSIS; STRAIN DIFFERENCE; SYNAPSE; WILD TYPE; ANIMAL EXPERIMENT; CELL DENSITY; CELL MATURATION; CEREBELLUM; GENOTYPE; NERVE CELL; NERVE CELL STIMULATION; PHENOTYPE;

ACTION POTENTIALS; AGING; ANIMALS; CELL LINE; CEREBELLUM; EXCITATORY POSTSYNAPTIC POTENTIALS; GENE DELETION; MICE; MUTAGENESIS, SITE-DIRECTED; N-METHYLASPARTATE; NEURONAL PLASTICITY; NEURONS; RECEPTORS, N-METHYL-D-ASPARTATE; SYNAPTIC TRANSMISSION; TISSUE DISTRIBUTION;

EID: 15844374168     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.2004.079467     Document Type: Article

References (27)

1. Bellocchio EE, Reimer RJ, Fremeau RT Jr & Edwards RH (2000). Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter. Science 289, 957-960.
2. Cathala L, Brickley S, Cull-Candy S & Farrant M (2003). Maturation of EPSCs and intrinsic membrane properties enhances precision at a cerebellar synapse. J Neurosci 23, 6074-6085.
3. Cathala L, Misra C & Cull-Candy S (2000). Developmental profile of the changing properties of NMDA receptors at cerebellar mossy fiber-granule cell synapses. J Neurosci 20, 5899-5905.
4. Chen L, Chetkovich DM, Petralia RS, Sweeney NT, Kawasaki Y, Wenthold RJ, Bredt DS & Nicoll RA (2000). Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms. Nature 408, 936-943.
5. Chen C & Okayama H (1987). High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7, 2745-2752.
6. Chen N, Ren J, Raymond LA & Murphy TH (2001). Changes in agonist concentration dependence that are a function of duration of exposure suggest N-methyl-D-aspartate receptor nonsaturation during synaptic stimulation. Mol Pharmacol 59, 212-219.
7. Cull-Candy S, Brickley S & Farrant M (2001). NMDA receptor subunits: diversity, development and disease. Curr Opin Neurobiol 11, 327-335.
8. 2+ block in NMDA receptor channels. J Neurosci 16, 3549-3558.
9. Leao RM, Mellor JR & Randall AD (2000). Tonic benzodiazepine-sensitive GABAergic inhibition in cultured rodent cerebellar granule cells. Neuropharmacology 39, 990-1003.
10. Losi G, Prybylowski K, Fu Z, Luo JH & Vicini S (2002). Silent synapses in developing cerebellar granule neurons. J Neurophysiol 87, 1263-1270.
11. Mott DD, Doherty JJ, Zhang S, Washburn MS, Fendley MJ, Lyuboslavsky P, Traynelis SF & Dingledine R (1998). Phenylethanolamines inhibit NMDA receptors by enhancing proton inhibition. Nat Neurosci 1, 659-667.
12. Ozaki M, Sasner M, Yano R, Lu HS & Buonanno A (1997). Neuregulin-beta induces expression of an NMDA-receptor subunit. Nature 390, 691-694.
13. Prybylowski K, Fu Z, Losi G, Hawkins LM, Luo J, Chang K, Wenthold RJ & Vicini S (2002). Relationship between availability of NMDA receptor subunits and their expression at the synapse. J Neurosci 22, 8902-8910.
14. Prybylowski K, Rumbaugh G, Wolfe BB & Vicini S (2000). Increased exon 5 expression alters extrasynaptic NMDA receptors in cerebellar neurons. J Neurochem 75, 1140-1146.
15. Rock DM & Macdonald RL (1992). The polyamine spermine has multiple actions on N-methyl-D-aspartate receptor single-channel currents in cultured cortical neurons. Mol Pharmacol 41, 83-88.
16. Rock DM & Macdonald RL (1995). Polyamine regulation of N methyl-D-aspartate receptor channels. Annu Rev Pharmacol Toxicol 35, 463-882.
17. Rumbaugh G, Prybylowski K, Wang JF & Vicini S (2000). Exon 5 and spermine regulate deactivation of NMDA receptor subtypes. J Neurophysiol 83, 1300-1306.
18. Rumbaugh G & Vicini S (1999). Distinct synaptic and extrasynaptic NMDA receptors in developing cerebellar granule neurons. J Neurosci 19, 10603-10610.
19. Silver RA, Traynelis SF & Cull-Candy SG (1992). Rapid-time-course miniature and evoked excitatory currents at cerebellar synapses in situ. Nature 355, 163-166.
20. Stocca G & Vicini S (1998). Increased contribution of NR2A subunit to synaptic NMDA receptors in developing rat cortical neurons. J Physiol 507, 13-24.
21. Studler B, Fritschy JM & Brunig I (2002). GABAergic and glutamatergic terminals differentially influence the organization of GABAergic synapses in rat cerebellar granule cells in vitro. Neuroscience 114, 123-133.
22. Takahashi T, Feldmeyer D, Suzuki N, Onodera K, Cull-Candy SG, Sakimura K & Mishina M (1996). Functional correlation of NMDA receptor epsilon subunits expression with the properties of single-channel and synaptic currents in the developing cerebellum. J Neurosci 16 4376-4382.
23. Takamori S, Rhee JS, Rosenmund C & Jahn R (2000). Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons. Nature 407, 189-194.
24. Townsend M, Yoshii A, Mishina M & Constantine-Paton M (2003). Developmental loss of miniature N-methyl-D-aspartate receptor currents in NR2A knockout mice. Proc Natl Acad Sci U S A 100, 1340-1345.
25. Vallano ML, Lambolez B, Audinat E & Rossier J (1996). Neuronal activity differentially regulates NMDA receptor subunit expression in cerebellar granule cells. J Neurosci 16, 631-639.
26. Vicini S, Wang JF, Li JH, Zhu WJ, Wang YH, Luo JH, Wolfe BB & Grayson DR (1998). Functional and pharmacological differences between recombinant N-methyl-D-aspartate receptors. J Neurophysiol 79, 555-566.
27. Wojcik SM, Rhee JS, Herzog E, Sigler A, Jahn R, Takamori S, Brose N & Rosenmund C (2004). An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size. Proc Natl Acad Sci U S A 101, 7158-7163.