Investigation of a hepatotoxicity screening system in primary cell cultures: -"What biomarkers would need to be addressed to estimate toxicity in conventional and new approaches?"-

Journal of Toxicological Sciences

Volumn 30, Issue 1, 2005, Pages 61-72

  Kikkawa, Rie ^a   Yamamoto, Toshinori ^a   Fukushima, Tamio ^a   Yamada, Hiroshi ^a   Horii, Ikuo ^a  

^a PFIZER INC   (United States)

Author keywords

Biomarker; Cytotoxicity; In vitro model; Primary monolayer culture; Proteomics; Rat hepatocytes

Indexed keywords

ACETYL COENZYME A ACETYLTRANSFERASE; ACYL COENZYME A DEHYDROGENASE; ALDEHYDE DEHYDROGENASE; ALPHA 1 ANTITRYPSIN; AMIODARONE; ARGININOSUCCINATE SYNTHASE; BIOLOGICAL MARKER; CARBON TETRACHLORIDE; DESMIN; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUTATHIONE PEROXIDASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GUANINE NUCLEOTIDE BINDING PROTEIN BETA SUBUNIT; HAPTOGLOBIN; HOMEODOMAIN PROTEIN; HOMEODOMAIN PROTEIN OTX1; LACTATE DEHYDROGENASE; LIPOCORTIN 2; ONCOPROTEIN; OROSOMUCOID; PARACETAMOL; PROPIONYL COENZYME A CARBOXYLASE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; PYRUVATE DEHYDROGENASE COMPLEX; RIBOSOME PROTEIN; TETRACYCLINE; THIOSULFATE SULFURTRANSFERASE; TRIOSEPHOSPHATE ISOMERASE; UNCLASSIFIED DRUG; UNINDEXED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; CYTOTOXICITY; IN VITRO STUDY; IN VIVO STUDY; LIVER CELL; LIVER TOXICITY; MALE; METABOLIC REGULATION; MITOCHONDRIAL RESPIRATION; NONHUMAN; OXIDATIVE STRESS; PROTEOMICS; RAT; RISK ASSESSMENT;

EID: 15744398882     PISSN: 03881350     EISSN: 18803989     Source Type: Journal    
DOI: 10.2131/jts.30.61     Document Type: Article

References (52)

1. Albano, E., Rundrgren, M., Harvison, P.J., Nelson, S.D. and Moldeus, P. (1985): Mechanisms of N-acetyl-p-benzo-quinone imine cytotoxicity. Mol. Pharmacol., 28, 306-311.
2. Alden, C.L., Sagartz, J.E., Smith, P.F., Wilson, A.G., Bunch, R.T. and Morris, D.L. (1999): The pathologist and toxicologist in pharmaceutical product discovery. Toxicol. Pathol., 27, 104-106.
3. Begue, J.M., Guguen-Guillouzo, C., Pasdeloup, N. and Guillouzo, A. (1984): Prolonged maintenance of active cytochrome P-450 in adult rat hepatocytes co-cultured with another liver cell type. Hepatology, 4, 839-842.
4. Boess, F., Kamber, M., Romer, S., Gasser, R., Muller, D., Albertini, S. and Suter, L. (2003): Gene expression in two hepatic cell lines, cultured primary hepatocytes, and liver slices compared to the in vivo liver gene expression in rats: Possible implications for toxicogenomics use of in vitro systems. Toxicol. Sci., 73, 386-402.
5. Breen, K.J., Schenker, S. and Heimberg, M. (1975): Fatty liver induced by tetracycline in the rat. Dose-response relationships and effect of sex. Gastroenterology, 69, 714-723.
6. Brewer, T. (1965): Tetracycline hepatotoxicity. Br. Med. J., 5440, 995.
7. Bugelski, P.J., Atif, U., Molton, S., Toeg, I., Lord, P.G. and Morgan, D.G. (2000): A strategy for primary high throughput cytotoxicity screening in pharmaceutical toxicology. Pharm. Res., 17, 1265-1272.
8. Chen, H.L., Wu, H.L., Fon, C.C., Chen, P.J., Lai, M.Y. and Chen, D.S. (1998): Long-term culture of hepatocytes from human adults, J. Biomed. Sci., 5, 435-440.
9. Chen, W., Koenigs, L.L., Thompson, S.J., Peter, R.M., Rettie, A.E., Trager, W.F. and Nelson, S.D. (1998): Oxidation of acetaminophen to its toxic quinone imine and nontoxic catechol metabolites by baculovirus-expressed and purified human cytochromes 2E1 and 2A6. Chem. Res. Toxicol., 11, 295-301.
10. Clothier, R.H., Humlme, L.M., Smith, M. and Balls, M. (1987): Comparison of the in vitro cytotoxicities and acute in vivo toxicities of 59 chemicals. Mol. Toxicol., 1, 571-577.
11. Dake, M.D., Madison, J.N., Montgomery, C.K., Shellito, J.E., Hinchcliffe, W.A., Winkler, M.L. and Bainton, D.F. (1985): Electron microscopic demonstration of lysosomal inclusion bodies in lung, liver, lymph nodes and blood leucocytes of patients with amiodarone pulmonary toxicity. Am. J. Med., 78, 506-512.
12. Dunn, J.C., Tompkins, R.G. and Yarmush, M.L. (1991): Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol. Prog., 7, 237-245.
13. Ekwall, B. and Acosta, D. (1982): In vitro comparative toxicity of selected drugs and chemicals in HeLa cells, Chang liver cells, and rat hepatocytes. Drug Chem. Toxicol., 5, 219-231.
14. Fromenty, B. and Pessayre, D. (1995): Inhibition of mitochondrial beta-oxidation as a mechanism of hepatotoxicity. Pharmacol. Ther., 67, 101-154.
15. Fry, J.R., Garle, M.J., Hammond, A.M. and Hatfield, A. (1990): Correlation of acute lethal potency with in vitro cytotoxicity. Toxicol. In Vitro, 4, 175-178.
16. Gant, T.W. (2003): Application of toxicogenomics in drug development., Drug News Perspect., 16, 217-221.
17. Garle, M.J., Fentem, J.H. and Fry, J.R. (1994): In vitro cytotoxicity tests for the prediction of acute toxicity in vivo. Toxicol. In Vitro, 8, 1303-1312.
18. Hamid, R., Rotshteyn, Y., Rabadi, L., Parikh, R. and Bullock, P. (2004): Comparison of alamar blue and MTT assays for high through-put screening. Toxicol. In Vitro, 18, 703-710.
19. Hart, S.J., Calder, I.C. and Tange, J.D. (1982): The metabolism and toxicity of paracetamol in Sprague-Dawley and Wister rats. Eur. J. Drug Metab. Pharmacokinet., 7, 203-222.
20. Holme, J.A., Dahlin, D.C., Nelson, S.D. and Dybing, E. (1984): Cytotoxic effects of N-acetyl-p-benzoquinone imine, a common arylating intermediate of paracetamol and N-hydroxyparacetamol., Biochem. Pharmacol., 33, 401-406.
21. Huhtala, A., Mannerstrom, M., Alajuuma, P., Nurmi, S., Toimela, T., Tahti, H., Salminen, L. and Uusitalo, H. (2002): Comparison of an immortalized human corneal epithelial cell line and rabbit corneal epithelial cell culture in cytotoxicity testing. J. Ocul. Pharmacol. Ther., 18, 163-175.
22. Ichihara, A., Nakamura, T., Tanaka, K., Tomita, Y., Aoyama, K., Kato, S. and Shinno, H. (1980): Biochemical functions of adult rat hepatocytes in primary culture. Ann. N. Y. Acad. Sci., 349, 77-78.
23. Kang, Y.H., Berthiaume, F., Nath, B.D. and Yarmush, M.L. (2004): Growth factors and nonparenchymal cell conditioned media induce mitogenic responses in stable long-term adult rat hepatocyte cultures. Exp. Cell Res., 293, 239-247.
24. Kilberg, M.S. (1982): Amino acid transport in isolated rat hepatocytes. J. Member. Biol., 69, 1-12.
25. Knowles, B.B., Howe, C.C. and Aden, D.P.(1980): Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen, Science, 209, 497-499.
26. Kola, I. and Landis, J., (2004): Can the pharmaceutical industry reduce attrition rates? Nat. Rev. Drug. Discov., 3, 711-716.
27. Kostrubsky, V.E., Strom, S.C., Hanson, J., Urda, E., Rose, K., Burliegh, J., Zocharski, P., Cai, H., Sinclair, J.F. and Sahi, J. (2003): Evaluation of hepatotoxic potential of drugs by inhibition of bile-acid transport in cultured primary human hepatocytes and intact rats. Toxicol. Sci., 76, 220-228.
28. LeCluyse, E.L., Audus, K.L. and Hochman, J.H. (1994): Formation of extensive canalicular networks by rat hepatocytes cultured in collagen-sandwich configuration. Am. J. Physiol., 266, C1764-1774.
29. Luber-Narod, J., Smith, B., Grant, W., Jimeno, J.M., Lopez-Lazaro, L. and Faircloth, G.T. (2001): Evaluation of the use of in vitro methodologies as tools for screening new compounds for potential in vivo toxicity. Toxicol. In Vitro, 15, 571-577.
30. Meador, V., Jordan, W. and Zimmermann, J. (2002): Increasing throughput in lead optimization in vivo toxicity screens. Curr. Opin. Drug Discov. Devel., 5, 72-78.
31. Mitchell, J.R., Jollow, D.J., Potter, W.Z., Davis, D.C., Gillette, J.R. and Brodie, B.B. (1973): Acetaminophen-induced hepatic necrosis. I. Role of drug metabolism. J. Pharmacol. Exp. Ther., 187, 185-194.
32. Moghe, P.V., Berthiaume, F., Ezzell, R.M., Toner, M., Tompkins, R.G. and Yarmush, M.L. (1996): Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials, 17, 373-385.
33. Nussler, A.K., Wang, A., Neuhaus, P., Fischer, J., Yuan, J., Liu, L., Zeilinger, K., Gerlach, J., Arnold, P.J. and Albrecht, W. (2001): The suitability of hepatocyte culture models to study various aspects of drug metabolism, ALTEX, 18, 91-101.
34. Paillard, F., Finot, F., Mouche, I., Prenez, A. and Vericat, J.A. (1999): Use of primary cultures of rat hepatocytes to predict toxicity in the early development of new chemical entities. Toxicol. In Vitro, 13, 693-700.
35. Patten, C.J., Thomas, P.E., Guy, R.L., Lee, M., Gonzalez, F.J., Guengerich, F.P. and Yang, C.S. (1993): Cytochrome P450 enzymes involved in acetaminophen activation by rat liver microsomes and their kinetics. Chem. Res. Toxicol., 6, 511-518.
36. Poucell, S., Ireton, J., Valencia-Mayoral, P., Downar, E., Larratt, L., Patterson, J., Blendis, L. and Phillips, M.J. (1984): Amiodarone-associated phospholipidosis and fibrosis of the liver: Light, immunochemical, and electron microscopic studies. Gastroenterology, 86, 926-936.
37. Recknagel, R.O., Glende, E.A. Jr., Dolak, J.A. and Waller, R.L. (1989): Mechanism of carbon tetrachloride toxicity. Pharmacol. Ther., 43, 139-154.
38. Riss, T.L. and Moravec, R.A. (2004): Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev. Technol., 2, 51-62.
39. Schenker, S. (1976): Tetracycline hepatotoxicity. A review. Mater. Med. Pol., 8, 173-176.
40. Seglen, P.O. (1976): Preparation of isolated rat liver cells. Methods Cell Biol., 13, 29-83.
41. 14C]-acetate uptake study in rat. Drug Chem. Toxicol., 25, 247-254.
42. Smet, K.D., Bruning, T., Blaszkewicz, M., Bolt, H.M., Vercruysse, A. and Rogiers, V. (2000): Biotransformation of trichloroethylene in collagen gel sandwich cultures of rat hepatocytes. Arch. Toxicol., 74, 587-592.
43. Somani, P., Bandyopadhyay, S., Klaunig, J.E. and Gross, S.A. (1990): Amiodarone- and desethylamiodarone-induced myelinoid inclusion bodies and toxicity in cultured rat hepatocytes. Hepatology, 11, 81-92.
44. Toritsuka, N., Nonogaki, Y., Hori, Y., Sawada, S., Shoun, H. and Motooka, S.(2001): Well maintained expression of CYP genes in sandwich-culturing hepatocytes: Quantitative analysis using real-time PCR method. Res. Commun. Mol. Pathol. Pharmacol., 110, 311-318.
45. th. ed., pp. 471-489, McGraw-Hill, New York.
46. Vandenberghe, J. (1996): Hepatotoxicology: structure, function and toxicological pathology. In Toxicology: principles and applications. (Niesink, R.J.M, Vries, J. and Hollinger, M.A., eds.), pp 669-700, CRC Press, New York.
47. Vandenberghe, Y., Glaise, D., Meyer, D.J., Guillouzo, A. and Ketterer, B. (1988): Glutathione transferase isoenzymes in cultured rat hepatocytes. Biochem. Pharmacol., 37, 2482-2485.
48. Viluksela, M., Vainio, P.J. and Tuominen, R.K. (1996): Cytotoxicity of macrolide antibiotics in a cultured human liver cell line. J. Antimicrob. Chemother., 38, 465-473.
49. Walker, T.M., Rhodes, P.C. and Westmoreland, C. (2000): The differential cytotoxicity of methotrexate in rat hepatocyte monolayer and spheroid cultures. Toxicol. In Vitro, 14, 475-485.
50. Wang, K., Shindoh, H., Inoue, T. and Horii, I. (2002): Advantages of in vitro cytotoxicity testing by using primary rat hepatocytes in comparison with established cell lines. J. Toxicol. Sci., 27, 229-237.
51. Wilkening, S., Stahl, F. and Bader, A. (2003): Comparison of primary human hepatocytes and hepatoma cell line HepG2 with regard to their biotransformation properties. Drug Metab. Dispos., 31, 1035-1042.
52. Yoshimura, I. (2003): In "Statistical Analysis of Toxicological Data", pp. 26-42, Scientist, Tokyo (in Japanese).