Well maintained expression of CYP genes in sandwich-culturing hepatocytes: Quantitative analysis using real-time PCR method

Research Communications in Molecular Pathology and Pharmacology

Volumn 110, Issue 5-6, 2001, Pages 311-318

  Toritsuka, Naoki ^a,b   Nonogaki, Yasushi ^a   Hori, Yuji ^a   Sawada, Shigeki ^a   Shoun, Hirofumi ^b,c   Motooka, Satoru ^a  

^a EISAI CO LTD   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; BETA ACTIN; COLLAGEN TYPE 1; CYTOCHROME P450 2B1; CYTOCHROME P450 2B2; CYTOCHROME P450 3A2; CYTOCHROME P450 3A23; CYTOCHROME P450 3A9; CYTOCHROME P450 ISOENZYME; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; DRUG METABOLISM; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; LIVER CELL; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; CELLS, CULTURED; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HEPATOCYTES; MALE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 0042808775     PISSN: 10780297     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Berthiaume, F., Moghe, P. V, Toner M. and Yarmush, M. L. (1996). Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: hepatocytes cultured in a sandwich configuration. FASEB J. 10, 1471-1484.
2. Bowen, W. P., Carey, J E, Miah A., McMurray, H. F., Munday, P. W., James, R. S., Coleman, R. A. and Brown, A. M. (2000) Measurement of cytochrome P450 gene induction in human hepatocytes using quantitative real-time reverse transcriptase-polymerase chain reaction. Drug Metab. Dispos. 28, 781-788.
3. Dunn, J. C., Yarmush, M. L., Koebe, H. G. and Tompkins, R. G. (1989). Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J. 3, 174-177.
4. Kem, A., Bader, A., Pichlmayr, R. and Sewing K. F. (1997). Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochem. Pharmacol. 54, 761-772.
5. Huss, J. M. and Kasper, C. B. (2000) Two-stage glucocorticoid induction of CYP3A23 through both the glucocorticoid and pregnane X receptors. Mol. Pharmacol. 58, 48-57.
6. Kocarek, T. A., Schuetz, E. G. and Guzelian, P. S. (1994) Biphasic regulation of cytochrome P450 2B1/2 mRNA expression by dexamethasone in primary cultures of adult rat hepatocytes maintained on matrigel. Biochem. Pharmacol. 48, 1815-1822.
7. LeCluyse, E. L, Bullock, P. L. and Parkinson, A. (1996). Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes. Adv. Drug Del. Rev. 22, 133-186.
8. Mahnke, A., Strotkamp, D., Roos, P. H., Hanstein, W. G., Chabot, G. G. and Nef, P. (1997). Expression and inducibility of cytochrome P450 3A9 (CYP3A9) and other members of the CYP3A subfamily in rat liver. Arch. Biochem. Biophys. 337, 62-68.
9. Pan, J., Xiang, Q. and Ball, S. (2000). Use of a novel real-time quantitative reverse transcription-polymerase chain reaction method to study the effects of cytokines on cytochrome P450 mRNA expression in mouse liver. Drug Metab. Dispos. 28, 709-713.
10. Sawada, S., Furihata, C. and Matsushima, T. (1989). In vivo short-term assays of repair and replication of rat liver DNA. J. Cancer Res. Clin. Oncol. 115, 345-350.