Multiplex PCR method for use in real-time PCR for identification of fish fillets from grouper (Epinephelus and Mycteroperca species) and common substitute species

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 6, 2005, Pages 2039-2045

  Trotta, Michele ^a,b   Schönhuth, Susana ^a   Pepe, Tiziana ^b   Cortesi, M Luisa ^b   Puyet, Antonio ^a   Bautista, José M ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

16S rRNA; Epinephelus spp.; Lates niloticus; Multiplex PCR; Mycteroperca spp.; Polyprion americanus; Real time PCR; Species identification

Indexed keywords

DNA; RNA 16S;

AMPLICON; ARTICLE; CROSS REACTION; DNA EXTRACTION; ELECTROPHORESIS; FISH; GENE AMPLIFICATION; MIGRATION; MITOCHONDRION; PERCH; REAL TIME POLYMERASE CHAIN REACTION; SPECIES; TECHNIQUE;

ANIMALS; DNA; FISH PRODUCTS; FISHES; PERCIFORMES; POLYMERASE CHAIN REACTION;

EPINEPHELUS; EPINEPHELUS AENEUS; EPINEPHELUS CANINUS; EPINEPHELUS COSTAE; EPINEPHELUS MARGINATUS; LATES NILOTICUS; MYCTEROPERCA; MYCTEROPERCA FUSCA; MYCTEROPERCA RUBRA; POLYPRION AMERICANUS;

EID: 15444371892     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf048542d     Document Type: Article

References (38)

1. Marko, P. B.; Lee, S. C.; Rice, A. M.; Gramling, J. M.; Fitzhenry, T. M.; McAalister, J. S.; Harper G. R.; Moran, A. L. Mislabelling of a depleted reef fish. Nature 2004, 430, 309-310.
2. Glazumina, B.; Skaramuca, B.; Glavic, N.; Kozul, V. Preliminary studies on reproduction and early stage rearing trial of dusky grouper, Epinephelus marginatus (Lowe, 1834). Aquacult. Res. 1998, 29, 769-771.
3. Asensio, L.; González, I.; Fernández, A.; Rodríguez, M. A.; Hernández, P. E.; García, T.; Martín, R. PCR-SSCP: a simple method for the authentication of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. J. Agric. Food Chem. 2001, 49, 1720-1723.
4. Lundstrom, R. Fish species identification by thin-layer polyacrylamide gel isoelectric focusing: Collaborative study. J. Assoc. Off. Anal. Chem. 1980, 63, 69-73.
5. Durand, P.; Landrein, A.; Quéro, J. C. Catalogue électrophorétique des poissons commerciaux; IFREMER: Nantes, France, 1985.
6. Etienne, M.; Jerome, M.; Fleurence, J.; Rehbein, H.; Kundiger, R.; Yman, I.; Ferm, M.; Craig, A.; Mackie, I.; Jessen, F.; Smelt, A.; Luten, J. A standardized method of identification of raw and heat-processed fish by urea isoelectric focusing: A collaborative study. Electrophoresis 1999, 20, 1923-1933.
7. Sotelo, C.; Gallardo, J. M.; Perez-Martin, R. I. Fish species identification in seafood products. Trends Food Sci. Technol. 1993, 4, 395-401.
8. Asensio, L.; Gonzalez, I.; Rodriguez, M. A.; Mayoral, B.; Lopez-Calleja, I.; Hernandez, P. E.; Garcia, T.; Martin, R. Identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets by polyclonal antibody-based enzyme-linked immunosorbent assay. J. Agric. Food Chem. 2003, 51, 1169-1172.
9. Barlett, S. E.; Davidson, W. S. FINS (Forensically Informative Nucleotide Sequencing): A procedure for identifying the animal origin of biological specimens. BioTechniques 1992, 12, 408-411.
10. Ferguson, A.; Taggart, J. B.; Prodöhl, P. A.; McMeel, O.; Thompson, C.; Stone, C.; McGinnity, P.; Hynes, R. A. The application of molecular markers to the study and conservation of fish populations, with special reference to Salmo. J. Fish Biol. 1995, 47 (Suppl. A), 103-126.
11. (11 ) Wolf, C.; Burgener, M.; Hübner, P.; Lüthy J. PCR-RFLP analysis of mitochondrial DNA: Differentiation of fish species. Lebensm.-Wiss. Technol. 2000, 33, 144-150.
12. Kocker, T. D.; Thomas, W. K.; Meyer, A.; Edwards, S. V.; Pääbo, S.; Villabianca, F. X.; Wilson, A. C. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. U.S.A. 1989, 86, 6196-6200.
13. Meyer, R.; Candrian, U. PCR-based DNA analysis for the identification and characterization of food components. Lebensm.-Wiss. Technol. 1996, 29, 1-9.
14. Mackie, I. M.; Pryde, S. E.; Gonzales-Sotelo, C.; Medina, I.; Peréz-Martín, R.; Quinteiro, J.; Rey-Mendez, M.; Rehbein, H. Challenges in the identification of species of canned fish. Trends Food Sci. Technol. 1999, 10, 9-14.
15. Sotelo, C. G.; Calo-Mata, P.; Chapela, Ma. J.; Pérez-Martin, R. I.; Rehbein, H.; Hold, G. L.; Russell, V. J.; Pryde, S. E.; Quinteiro, J.; Izquierdo, M.; Rey-Méndez, M.; Rosa, C.; Santos, A. T. Identification of flatfish (Pleuronectiforme) species using DNA-based techniques. J. Agric. Food Chem. 2001, 49, 4562-4569.
16. Jerome, M.; Lemaire, C.; Bautista, J. M.; Fleurence, J.; Etienne, M. Molecular phylogeny and species identification of sardines. J. Agric. Food Chem. 2003, 51, 43-50.
17. Hold, G. L.; Russell, V. J.; Pryde, S. E.; Rehbein, H.; Quinteiro, J.; Vidal, R.; Rey-Mendez, M.; Sotelo, C.; Pérez-Martin, R. I.; Santos, A. T.; Rosa, C. Development of a DNA-Based method aimed to identifying the fish species in food products. J. Agric. Food Chem. 2001, 49, 1175-1179.
18. Karaiskou, N.; Triantafyllidis, A.; Triantafyllidis, C. Discrimination of three Trachurus species using both mitochondrial- and nuclear DNA approaches. J. Agric. Food Chem. 2003, 51, 4935-4940.
19. Sebastio, P.; Zanelli, P.; Neri, T. M. Identification of anchovy (Engraulis encrasicholus L.) and gilt sardine (Sardinella aurita) by polymerase chain reaction, sequence of their mitochondrial cytochrome b gene, and restriction analysis of polymerase chain reaction products in semipreserves. J. Agric. Food Chem. 2001, 49, 1194-1199.
20. Zardoya, R.; Garrido-Pertierra, A.; Bautista, J. M. The complete nucleotide sequence of the mitochondrial DNA genome of the rainbow trout, Oncorhynchus mykiss. J. Mol. Evol. 1995, 41 (6), 942-951.
21. Miya, M.; Takeshima, H.; Endo, H.; Ishiguro, N. B.; Inoue, J. G.; Mukai, T.; Satoh, T. P.; Yaraaguchi, M.; Kawaguchi, A.; Mabuchi, K.; Shirai, S. M.; Nishida, M. Major patterns of higher teleostean phylogenies: a new perspective based on 100 complete mitochondrial DNA sequences. Mol. Phylogenet. Evol. 2003, 26, 121-138.
22. Gilles, A.; Miquelis, A.; Quignard, J. P.; Faure, É. Molecular phylogeography of western Mediterranean dusky grouper Epinephelus marginatus. C. R. Acad. Sci. Paris, Sci. 2000, 323, 195-205.
23. Quinteiro, J.; Sotelo, C. G.; Rehbein, H.; Pryde, S. E.; Medina, I.; Pérez-Martin, R. I.; Rey-Méndez, M.; Mackie, I. M. Use of mtDNA direct polymerase chain reaction (PCR) sequencing and PCR-restriction fragment length polymorphism methodologies in species identification of canned tuna. J. Agric. Food Chem. 1998, 46, 1662-1669.
24. Ram, J. L.; Ram, M. L.; Baidoun, F. F. Authentication of canned tuna and bonito by sequence and restriction site analysis of Polymerase Chain Reaction products of mitochondrial DNA. J. Agric. Food Chem. 1996, 44, 2460-2467.
25. Asensio, L.; González, I.; Fernández, A.; Céspedes, A.; Hernández, P. E.; García, T.; Martín, R. Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza) and wreck fish (Polyprion americanus) by Polymerase Chain Reaction-restriction fragment length polymorphism of a 12S rRNA gene fragment. J. Food Prot. 2000, 63, 1248-1252.
26. Asensio, L.; Gonzalez, I.; Fernandez, A.; Rodriguez, M. A.; Lobo E.; Hernandez, P.; Garcia, T.; Martin, R. Application of Random Amplified Polymorphic DNA (RAPD) analysis for identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. J. Food Prot. 2002, 65, 432-435.
27. Matsunaga, T.; Chikuni, K.; Tanabe, R.; Muroya, S.; Shibata, K.; Yamada, J.; Shinmura, Y. A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci. 1999, 51, 143-148.
28. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
29. Palumbi, S. Nucleic acids II: The polymerase chain reaction. In Molecular Systematics; Hillis, D. M., Moritz, C., Mable, B. K., Eds.; Sinauer: Sunderland, MA, 1996.
30. Thompson, J. D.; Gibson, T. J.; Plewniak, F.; Jeanmougin, F.; Higgins, D. G. The Clustal_X windows interface: flexible strategies for multiple sequences alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25, 4876-4882.
31. Wittwer, C. T.; Herrmann, M. G.; Gundry, C. N.; Elenitoba-Johnson, K. S. J. Real-time multiplex PCR assays. Methods 2001, 25, 430-442.
32. Asensio, L.; Gonzalez, I.; Rodríguez, M. A.; Hernández, P. E.; Garcia, T.; Martín, R. PCR-ELISA for the semiquantitative detection of Nile perch (Lates niloticus) in sterilized fish muscle mixtures J. Agric. Food Chem. 2004, 52, 4419-4422.
33. Sotelo, C. G. Instituto de Investigaciones Marinas (CSIC) Development of an identification and quantitation system for cod (Gadus morhua) using Taqman assay. Presented at the 33rd WEFTA Meeting and 48th Atlantic Fisheries Technology Conference, June 11-14, 2003, Reykkjavik, Iceland.
34. Wang, G.; Clark, C. G.; Rodgers, F. G. Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR. J. Clin. Microbiol. 2002, 40, 3613-3619.
35. Fukushima, H.; Tsunomori, Y.; Seky, R. Duplex Real-Time SYBR Green PCR assay for detection of 17 species food- or waterborne pathogens in stools. J. Clin. Microbiol. 2003, 5134-5146.
36. LaGier, M. J.; Joseph, L. A.; Passaretti, T. V.; Musser, K. A.; Cirino, N. M. A Real-time multiplexed PCR assay for rapid detection and differentiation of Campylobacter jejuni and Campylobacter coli. Mol. Cell. Probes 2004, 18, 4, 275-82.
37. Suarez, P.; Zardoya, R.; Prieto, C.; Solana, A.; Tabares, E.; Bautista, J. M.; Castro, J. M. Direct-detection of the porcine reproductive and respiratory syndrome (PRRS) virus by reverse polymerase chain-reaction (RT-PCR). Arch. Virol. 1994, 135 (1-2), 89-99.
38. Beuret, C. Simultaneous detection of enteric viruses by multiplex real-time RT-PCR. J. Virol. Methods 2004, 115, 1-8.