Suppression of bcl-2 gene by RNA interference increases chemosensitivity to cisplatin in nasopharyngeal carcinoma cell line CNE1

Acta Biochimica et Biophysica Sinica

Volumn 36, Issue 11, 2004, Pages 749-753

  Yin, Zhi Hua ^a   Ren, Cai Ping ^a   Li, Feng ^a   Yang, Xu Yu ^a   Li, Hui ^a   Zhao, Ming ^a   Yao, Kai Tai ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

bcl 2; Cisplatin; Nasopharyngeal carcinoma; RNA interference

Indexed keywords

CISPLATIN; PROTEIN BCL 2; SHORT HAIRPIN RNA;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CHEMOSENSITIVITY; CONTROLLED STUDY; FLOW CYTOMETRY; GENE REPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NASOPHARYNX CARCINOMA; NITROBLUE TETRAZOLIUM TEST; PROTEIN EXPRESSION; RNA INTERFERENCE; WESTERN BLOTTING;

EID: 15444364279     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: 10.1093/abbs/36.11.749     Document Type: Article

References (35)

1. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 1998, 391: 806-811
2. Clemens JC, Worby CA, Simonson-Leff N, Muda M, Maehama T, Hemmings BA, Dixon JH. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Natl Acad Sci USA, 2000, 97: 6499-6503
3. Hammond SM, Bernstein E, Beach D, Hannon GJ. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature, 2000, 404: 293-296
4. Ngo H, Tschudi C, Gull K, Ullu E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc Natl Acad Sci USA, 1998, 95: 14687-14692
5. Sanchez-Alvarado A, Newmark PA. Double-stranded RNA specifically disrupt gene expression during planarian regeneration. Proc Natl Acad Sci USA, 1999, 96: 5049-5054
6. Fagard M, Boutet S, Morel JB, Bellini C, Vaucheret H. AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals. Proc Natl Acad Sci USA, 2000, 97(21): 11650-11654
7. Hunter T, Hunt T, Jackson RJ, Robertson HD. The characteristics of inhibition of protein synthesis by double-stranded ribonucleic acid in reticulocyte lysates. J Biol Chem, 1975, 250: 409-417
8. Billy E, Brondani V, Zhang H, Muller U, Filipowicz W. Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines. Proc Natl Acad Sci USA, 2001, 98(25): 14428-14433
9. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 2001, 411(6836): 428-429
10. Miyagishi M, Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat Biotechnol, 2002, 20(5): 497-500
11. Paul CP, Good PD, Winer I, Engelke DR. Effective expression of small interfering RNA in human cells. Nat Biotechnol, 2002, 20(5): 505-508
12. Sui G, Soohoo C, Affar el B, Gay F, Shi Y, Forrester WC, Shi Y. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA, 2002, 99(8): 5515-5520
13. McCaffrey AP, Meuse L, Pham TT, Conklin DS, Hannon GJ, Kay MA. RNA interference in adult mice. Nature, 2002, 418: 38-39
14. Tiscornia G, Singer O, Ikawa M, Verma IM. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci USA, 2003, 100(4): 1844-1848
15. Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang E, Kopinja J, Ronney DE et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet, 2003, 33(3): 401-406
16. Nicolson NL, Talpaz M, Nicolson GL. Chromatin nucleoprotein complexes containing tightly bound c-abl, p53 and bcl-2 gene sequences: Correlation with progression of chronic myelogenous leukemia. Gene, 1996, 169(2): 173-178
17. Sarac S, Akyol MU, Kanbur B, Poyraz A, Akyol G, Yilmaz T, Sungur A. Bcl-2 and LMP1 expression in nasopharyngeal carcinomas. Am J Otolaryngol, 2001, 22(6): 377-382
18. Khabir A, Ghorbel A, Daoud J, Frikha M, Drira MM, Laplanche A, Busson P et al. Similar BCL-X but different BCL-2 levels in the two age groups of North African nasopharyngeal carcinomas. Cancer Detect Prev, 2003, 27(4): 250-255
19. Sheu LF, Chen A, Meng CL, Ho KC, Lin FG, Lee WH. Analysis of bcl-2 expression in normal,inflamed, dysplastic nasopharygeal epithelia and nasopharyngeal carcinoma: Association with p53 expression. Hum Pathol, 1997, 28(5): 556-562
20. Gross A, McDonnell JM, Korsmeyer SJ. BCL-2 family members and the mitochondria in apoptosis. Genes Dev, 1999, 13: 1899-1911
21. Rudin CM, Thompson CB. Apoptosis and disease: Regulation and clinical relevance of programmed cell death. Annu Rev Med, 1997, 48: 267-281
22. Reed JC. Bcl-2 family proteins: Regulators of chemoresistance in cancer. Toxicol Lett, 1955, 82-83: 155-158
23. Lotem J, Sachs L. Regulation by bcl-2, c-myc, and p53 of susceptibility to induction of apoptosis by heat shock and cancer chemotherapy compounds in differentiation-competent and -defective myeloid leukemic cells. Cell Growth Differ, 1993, 4: 41-47
24. Dole M, Nunez G, Merchant AK, Maybaum J, Rode CK, Bloch CA, Castle VP. Bcl-2 inhibits chemotherapy-induced apoptosis in neuroblastoma. Cancer Res, 1994, 54: 3253-3259
25. Li MT, Sun J, Qiu PX, Yan GM. Mitochondrial mechanisms of bcl-2 on ionizing radiation-induced apoptosis. Acta Biochim Biophys Sin, 1997, 29(5): 495-502
26. Zhang J, Peng WD, Hui HX, Jin M, Wang DN, Wang CJ, Qian HW. Inducible expression of bcl-2 antisense RNA promotes apoptosis of human gastric cancer cell line. Acta Biochim Biophys Sin, 1999, 31(3): 279-283
27. Zhang Y, Lei XY. Effect of bcl-2 antisense oligodeoxynucleotides on drug sensitivity of leukemic cells. Hematol J, 2003, 4(3): 187-197
28. Lopes de Menezes DE, Hu Y, Mayer LC. Combined treatment of Bcl-2 antisense oligodeoxynucleotides (G3139), p-glycoprotein inhibitor (PSC833), and sterically stabilized liposomal doxorubicin suppresses growth of drug-resistant growth of drug-resistant breast cancer in severely combined immunodeficient mice. J Exp Ther Oncol, 2003, 3(2): 72-82
29. Rudin CM, Kozloff M, Hoffman PC, Edelman MJ, Karnauskas R, Tomek R, Szeto L et al. Phase I study of G3139, a bcl-2 antisense oligonucleotide, combined with carboplatin and etoposide in patients with small-cell lung cancer. J Clin Oncol, 2004, 22(6): 1110-1117
30. Luzi E, Papucci L, Schiavone N, Donnini M, Lapucci A, Tempestini A, Witort E et al. Downregulation of bcl-2 expression in lymphoma cells by bcl-2 ARE-targeted modified, synthetic ribozyme. Cancer Gene Ther, 2003, 10(3): 201-208
31. Perlman H, Sata M, Krasinski K, Dorai T, Buttyan R, Walsh K.Adenovirus-encoded hammerhead ribozyme to Bcl-2 inhibits neointimal hyperplasia and induces vascular smooth muscle cell apoptosis. Cardiovasc Res, 2000, 45(3): 570-578
32. Gibson SA, Pellenz C, Hutchison RE, Davey FR, Shillitoe EJ. Induction of apoptosis in oral cancer cells by an anti-bcl-2 ribozyme delivered by an adenovirus vector. Clin Cancer Res, 2000, 6(1): 213-222
33. Dorai T, Goluboff ET, Olsson CA, Buttyan R. Development of a hammerhead ribozyme against BCL-2. II. Ribozyme treatment sensitizes hormone-resistant prostate cancer cells to apoptotic agents. Anticancer Res, 1997, 17 (5A): 3307-3312
34. Cioca DP, Aoki Y, Kiyosawa K. RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines. Cancer Gene Ther, 2003, 10(2): 125-133
35. Lima RT, Martins LM, Guimaraes JE, Sambade C, Vasconcelos MH. Specific downregulation of bcl-2 and xIAP by RNAi enhances the effects of chemotherapeutic agents in MCF-7 human breast cancer cells. Cancer Gene Ther, 2004, 11(5): 309-316