Detection of radiation-induced apoptosis using the comet assay

Journal of Veterinary Medical Science

Volumn 65, Issue 11, 2003, Pages 1161-1166

  Wada, Seiichi ^a,b   Van Khoa, Tran ^d   Kobayashi, Yasuhiko ^a   Funayama, Tomoo ^a   Yamamoto, Kazuo ^a,c   Natsuhori, Masahiro ^b   Ito, Nobuhiko ^b  

^a Japan Atomic Energy Research Institute   (Japan)

^b KITASATO UNIVERSITY   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

^d Department of Biology and Medical Geneties   (Viet Nam)

Author keywords

Apoptosis; Comet assay; DNA fragment; Gamma ray; TUNEL assay

Indexed keywords

ANIMAL; APOPTOSIS; ARTICLE; CELL CULTURE; COMET ASSAY; COMPARATIVE STUDY; FLUORESCENCE MICROSCOPY; GAMMA RADIATION; METHODOLOGY; NICK END LABELING; PHYSIOLOGY; RADIATION EXPOSURE;

ANIMALS; APOPTOSIS; CELLS, CULTURED; COMET ASSAY; GAMMA RAYS; IN SITU NICK-END LABELING; MICROSCOPY, FLUORESCENCE;

EID: 1542678932     PISSN: 09167250     EISSN: None     Source Type: Journal    
DOI: 10.1292/jvms.65.1161     Document Type: Article

References (34)

1. Bacsó, Z. and Eliason, J. E. 2001. Measurement of DNA damage associated with apoptosis by laser scanning cytometry. Ctyometry 45: 180-186.
2. Bacsó, Z., Everson, R. B. and Eliason, J. F. 2000. The DNA of Annexin V-binding apoptotic cell is highly fragmented. Cancer Res. 60: 4623-4628.
3. Bock, C., Dube, A., Greulich, K. O. and Gupta, P. K. 1999. Identification by microscopically controlled comet assay of peritoneal macrophages in a mixture of peritoneal exudate for DNA strand break analysis. Mutat. Res. 439: 171-181.
4. Cregan, S. P., Smith, B. P., Brown, D. L. and Mitchel, R. E. J. 1999. Two pathways for the induction of apoptosis in human lymphocytes. Int. J. Radiat. Biol. 75: 1069-1086.
5. Cregan, S. P., Boreham, D. R., Walker, P. R., Brown, D. L. and Michel, R. E. J. 1994. Modification of radiation-induced apoptosis in radiation-or hyperthemia-adapted human lymphocytes. Biochem. Cell Biol. 72: 475-482.
6. Dikomey, E., Dahm-daphi, J., Brammer, I., Martensen, R. and Kaina, B. 1998. Correlation between cellular radiosensitivity and non-repaired double-strand breaks studied in nine mammalian cell lines. Int. J. Radiat. Biol. 73: 269-278.
7. Fairbairn, D. W., Walburger, D. K., Fairbairn, J. J. and O'Neill, K. L. 1996. Key morphologic changes and DNA strand breaks in human lymphoid cells: Discriminating apoptosis from necrosis. Scanning 18: 407-416.
8. Fujita, K., Kawarada, Y., Terada, K., Sugiyama, T., Ohyama, H. and Yamada, T. 2000. Quantitative detection of apoptotic thymocytes in low-dose X-irradiated mice by an anti-single-stranded DNA antibody. J. Radiat. Res. 41: 139-149.
9. Godard, T., Deslandes, E., Lebailly, P., Vigreux, C., Sichel, F., Poul, J. M. and Ganuduchon, P. 1999. Early detection of stauroaporone-induced apoptosis by comet assay and Annexin V assays. Histochem. Cell Biol. 112: 155-161.
10. Godard, T., Deslandes, E., Lebailly, P., Vigreux, C., Poullain, L., Sichel, F., Poul, J. M. and Gauduchon, P. 1999. Comet assay and DNA flow cytometry analysis of staurosporine-induced apoptosis. Cytometry 36: 117-122.
11. Gopalakrishna, P. and Khar, A. 1995. Comet assay to measure DNA damage in apoptotic cells. J. Biochem. Biophys. Methods 30: 69-73.
12. Gorczyca, W., Gong, J. and Darzynkiewicz, Z. 1993. Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53: 1945-1951.
13. Henderson, L., Wolfreys, A., Fedyk, J., Bourner, C. and Windebank, S. 1998. The ability of the comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis 13: 89-94.
14. Humar, B., Muller, H. and Scott, R. J. 1997. Elevated frequency of p53-independent apoptosis after irradiation increases levels of DNA breaks in ataxia telangiectasia lymphoblasts. Int. J. Radiat. Biol. 72: 257-269.
15. Kindzelskii, A. L. and Petty, H. R. 1999. Ultrasensitive detection of hydrogen peroxide-mediated DNA damage after alkaline single cell gel electrophoresis using occultation microscopy and TUNEL labeling. Mutat. Res. 426: 11-22.
16. Kizilian, N., Wilkins, R. C., Reinhardt, P., Ferrarotto, C., Mclean, J. R. N. and Mcnamee, J. P. 1999. Silver-stained comet assay for detection of apoptosis. Biotechniques 27: 926-930.
17. Kockx, M. M., Muhring, J., Knaapen, M. W. M. and de Meyer, G. R.Y. 1998. RNA synthesis and splicing interferes with DNA in situ end labeling techniques used to detect apoptosis. Am. J. Pathol. 152: 885-888.
18. McKelvey-Martin, V. J., Ho, E. T. S., McKeown, S. R., Johnston, S. R., McCarthy, P. J., Rajab, N. E. and Downes, C. S. 1998. Emerging applications of the single cell gel electrophoresis (comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization comets for identification of damaged and repaired DNA sequence in individual cells. Mutagenesis 13: 1-8.
19. O'Callaghan, Y. C., Woods, J. A. and O'Brien, N. M. 2001. Limitations of the single-cell gel electrophoresis assay to monitor apoptosis in U937 and HepG2 cells exposed to 7 β-hydroxycholesterol. Biochem. Phamacol. 61: 1217-1226.
20. Ohyama, H., Tamamoto, H. and Yanada, T. 1998. "Teardrop assay," a rapid and sensitive method for characterization of radiation-induced apoptotic DNA fragmentation in individual thymocytes. pp. 185-198. In: Apoptosis: Its Role and Mechanism. Business Center for Academic Societies.
21. Olive, P. L. 1999. Review. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol. 75: 395-405.
22. Olive, P. L. and Banath, J. P. 1995. Sizing highly fragmented DNA in individual apoptotic cells using the comet assay and a DNA crosslinking agent. Exp. Cell Res. 221: 19-26.
23. Olive, P. L., Frazer, G. and Banáth, J. P. 1993. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res. 36: 130-136.
24. Olive, P. L., Johnston, P. J., Banath, J. P. and Durand, R. E. 1998. The comet assay: A new method to examine heterogeneity associated with solid tumors. Nat. Med. 4: 103-105.
25. Robert, S. M., Chen, B., Eric, W. A. and Joseph, L. R. 1998. Detection of DNA damage by the alkaline comet assay after exposure to low-dose gamma radiation. Radiat. Res. 149: 396-400.
26. Roser, S., Pool-Zobel, B. L. and Rechkemmer, G. 2001. Contribution of apoptosis to responses in the comet assay. Mutat. Res. 497: 169-175.
27. Santos, S. J., Singh, N. P. and Natarajan, A. T. 1997. Fluorescence in situ hybridization with comets. Exp. Cell Res. 232: 407-411.
28. Singh, N. P. 2000. A simple method for accurate estimation of apoptotic cells. Exp. Cell Res. 256: 328-337.
29. Singh, N. P. 2000. Microgel for estimation of DNA strand breaks, DNA protein crosslink and apoptosis. Mutat. Res. 455: 111-127.
30. Singh, N. P., Stephens, R. E., Singh, H. and Lai, H. 1999. Visual quantification of DNA double-strand breaks in bacteria. Mutat. Res. 429: 159-168.
31. Siles, E., Villalobos, M., Jones, L., Guerrero, R., Eady, J. J., Valenzuela, M. T., Núnez, M. I., Mcmillan, T. J. and Ruiz de Almodo, J. M. 1998. Apoptosis after gamma irradiation, Is it an important cell death modality? Br. J. Cancer 78: 1594-1599.
32. Tateyama, H., Tada, T., Hattori, H., Murase, T., Li, W. X. and Eimoto, T. 1998. Effects of prefixation and fixation times on apoptosis detection by in situ end-labeling of fragmented DNA. Arch. Pathol. Lab. Med. 122: 252-255.
33. Tauchi, H. and Sawada, S. 1994. Analysis of mitotic cell death caused by radiation in mouse leukaemia L5178Y cells: apoptosis is the ultimate form of cell death following mitotic failure. Int. J. Radiat. Biol. 65: 449-455.
34. Wilkins, R. C., Wilkinson, D., Maharaj, H. P., Bellier, P. V., Cybulski, M. B. and McLean, J. R. N. 2002. Differential apoptotic response to ionizing radiation in subpopulations human white blood cells. Mutat. Res. 513: 27-36.