Interconversion of the androstenedione hydroxylase specificities of cytochromes P450 2B4 and 2B5 upon simultaneous site-directed mutagenesis of four key substrate recognition residues

Archives of Biochemistry and Biophysics

Volumn 335, Issue 1, 1996, Pages 152-160

  He, You Qun ^a   Szklarz, Grazyna D ^a   Halpert, James R ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

cytochrome P450; heterologous expression; molecular modeling; mutagenesis; steroid hydroxylation; structure function relationships

Indexed keywords

ANDROSTENEDIONE; CYTOCHROME P450; STEROID MONOOXYGENASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; HYDROXYLATION; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

ESCHERICHIA COLI;

EID: 0030297153     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1996.0493     Document Type: Article

References (31)

1. 1. Nelson, D. R., Kamataki, T., Waxman, D. J., Guengerich, F. P., Estabrook, R. W., Feyereisen, R., Gonzalez, F. J., Coon, M. J., Gunsalus, I. C., Gotoh, O., Okuda, K., and Nebert, D. W. (1993) DNA Cell Biol. 12, 1-51.
2. 2. Lindberg, R. L. P., and Negishi, M. (1989) Nature 339, 632-634.
3. 3. Iwasaki, M., Darden, T. A., Pedersen, L. G., and Negishi, M. (1995) Biochemistry 34, 5054-5059.
4. 4. Iwasaki, M., Darden, T. A., Pedersen, L. G., Davis, D. G., Juvonen, R. O., Sueyoshi, T., and Negishi, M. (1993) J. Biol. Chem. 268, 759-762.
5. 5. Aoyama, T., Korzekwa, K., Nagata, K., Adesnik, M., Reiss, A., Lapenson, D. P., Gillette, J., Gelboin, H. V., Waxman, D. J., and Gonzalez, F. J. (1989) J. Biol. Chem. 264, 21327-21333.
6. 6. Kedzie, K. M., Balfour, C. A., Escobar, G. Y., Grimm, S. W., He, Y. A., Pepperl, D. J., Regan, J. W., Stevens, J. C., and Halpert, J. R. (1991) J. Biol. Chem. 266, 22515-22521.
7. 7. He, Y. A., Balfour, C. A., Kedzie, K. M., and Halpert, J. R. (1992) Biochemistry 31, 9220-9226.
8. 8. Halpert, J. R., and He, Y. A. (1993) J. Biol. Chem. 268, 4453-4457.
9. 9. He, Y. A., Luo, Z., Klekotka, P. A., Burnett, V. L., and Halpert, J. R. (1994) Biochemistry 33, 4419-4424.
10. 10. Hasler, J. A., Harlow, G. R., Szklarz, G. D., John, G. H., Kedzie, K. M., Burnett, V. L., He, Y. A., Kaminsky, L. S., and Halpert, J. R. (1994) Mol. Pharmacol. 46, 338-345.
11. 11. Szklarz, G. D., He, Y. A., and Halpert, J. R. (1995) Biochemistry 34, 14312-14322.
12. 12. Szklarz, G. D., He, Y. Q., Kedzie, K. M., Halpert, J. R., and Burnett, V. L. (1996) Arch. Biochem. Biophys. 327, 308-318.
13. 13. Liu, J., He, Y. A., and Halpert, J. R. (1996) Arch. Biochem. Biophys. 327, 167-173.
14. 14. Kronbach, T., and Johnson, E. F. (1991) J. Biol. Chem. 266, 6215-6220.
15. 15. Kronbach, T., Kemper, B., and Johnson, E. F. (1991) Biochemistry 30, 6097-6102.
16. 16. Straub, P., Lloyd, M., Johnson, E. F., and Kemper, B. (1993) J. Biol. Chem. 268, 21997-22003.
17. 17. Imai, Y., and Nakamura, M. (1989) Biochem. Biophys. Res. Commun. 158, 717-722.
18. 18. Hsu, M. H., Griffin, K. J., Wang, Y., Kemper, B., and Johnson, E. F. (1993) J. Biol. Chem. 268, 6939-6944.
19. 19. Kaminsky, L. S., de Morais, S. M. F., Faletto, M. B., Dunbar, D. A., and Goldstein, J. A. (1992) Mol. Pharmacol. 43, 234-239.
20. 20. Straub, P., Lloyd, M., Johnson, E. F., and Kemper, B. (1994) Biochemistry 33, 8029-8034.
21. 21. Matsunaga, E., Zeugin, T., Zanger, U. M., Aoyama, T., Meyer, U. A., and Gonzalez, F. J. (1990) J. Biol. Chem. 265, 17197-17201.
22. 22. Fukuda, T., Imai, Y., Komori, M., Nakamura, M., Kusunose, E., Satouchi, K., and Kusunose, M. (1993) J. Biol. Chem. 113, 7-12.
23. 23. Gotoh, O. (1992) J. Biol. Chem. 267, 83-90.
24. 24. Gasser, R., Negishi, M., and Philpot, R. M. (1988) Mol. Pharmacol. 32, 22-30.
25. 25. Kedzie, K. M., Philpot, R. M., and Halpert, J. R. (1991) Arch. Biochem. Biophys. 291, 176-186.
26. 26. Stemmer, W. P., Morris, S. K., and Wilson, B. S. (1993) Biotechniques 14, 256-265.
27. 27. John, G. H., Hasler, J. A., He, Y. A., and Halpert, J. R. (1994) Arch. Biochem. Biophys. 314, 367-375.
28. 28. Omura, T., and Sato, R. (1964) J. Biol. Chem. 239, 2379-2387.
29. 29. He, Y. Q., He, Y. A., and Halpert, J. R. (1995) Chem. Res. Toxicol. 8, 574-579.
30. 30. Szklarz, G. D., Ornstein, R. L., and Halpert, J. R. (1994) J. Biomol. Struct. Dyn. 12, 61-78.
31. 31. Johnson, E. F. (1992) Trends Pharmacol. Sci. 13, 122-126.