Elucidation of amino acid residues critical for unique activities of rabbit cytochrome P450 2B5 using hybrid enzymes and reciprocal site-directed mutagenesis with rabbit cytochrome P450 2B4

Archives of Biochemistry and Biophysics

Volumn 327, Issue 2, 1996, Pages 308-318

  Szklarz, Grazyna D ^a   He, You Qun ^a   Kedzie, Karen M ^a,b   Halpert, James R ^a   Burnett, Vicki L ^a,c  

^a UNIVERSITY OF ARIZONA   (United States)

^b ALLERGAN INC   (United States)

^c PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

Author keywords

cytochrome P450; heterologous expression; molecular modeling; mutagenesis; steroid hydroxylation; structure function relationships

Indexed keywords

CYTOCHROME P450 ISOENZYME; HYBRID PROTEIN; MUTANT PROTEIN;

AMINO ACID COMPOSITION; AMINO ACID SUBSTITUTION; ARTICLE; COMPUTER MODEL; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABBIT; SITE DIRECTED MUTAGENESIS; STEROID HYDROXYLATION;

EID: 0342679092     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1996.0127     Document Type: Article

References (29)

1. Nelson, D. R., Kamataki, T., Waxman, D. J., Guengerich, F. P., Estabrook, R. W., Feyereisen, R., Gonzalez, F. J., Coon, M. J., Gunsalus, I. C., Gotoh, O., Okuda, K., and Nebert, D. W. (1993) DNA Cell Biol. 12, 1-51.
2. Guengerich, F. P. (1993) Drug Metab. Dispos. 21, 1-6.
3. Waxman, D. J. (1988) Biochem. Pharmacol. 37, 71.
4. Kedzie, K. M., Balfour, C. A., Escobar, G. Y., Grimm, S. W., He, Y.-A., Pepperl, D. J., Regan, J. W., Stevens, J. C., and Halpert, J. R. (1991) J. Biol. Chem. 266, 22515-22521.
5. He, Y.-A., Balfour, C. A., Kedzie, K. M., and Halpert, J. R. (1992) Biochemistry 31, 9220-9226.
6. Halpert, J. R., and He, Y.-A. (1993) J. Biol. Chem. 268, 4453-4457.
7. He, Y.-A., Luo, Z., Klekotka, P. A., Burnett, V. L., and Halpert, J. R. (1994) Biochemistry 33, 4419-4424.
8. Luo, Z., He, Y.-A., and Halpert, J. R. (1994) Arch. Biochem. Biophys. 309, 52-57.
9. He, Y. Q., He, Y. A., and Halpert, J. R. (1995) Chem. Res. Toxicol. 8, 574-579.
10. Szklarz, G. D., He, Y. A., and Halpert, J. R. (1995) Biochemistry 34, 14312-14322.
11. Szklarz, G. D., Ornstein, R. L., and Halpert, J. R. (1994) J. Biomol. Struct. Dyn. 12, 61-78.
12. Kedzie, K. M., Grimm, S. W., Chen, F., and Halpert, J. R. (1993) Biochim. Biophys. Acta 1164, 124-132.
13. Liu, J., He, Y. A., and Halpert, J. R. (1996) Arch. Biochem. Biophys., in press.
14. Hasler, J. A., Harlow, G. R., Szklarz, G. D., John, G. H., Kedzie, K. M., Burnett, V. L., He, Y. A., Kaminsky, L. S., and Halpert, J. R. (1994) Mol. Pharmacol. 46, 338-345.
15. Kedzie, K. M., Philpot, R. M., and Halpert, J. R. (1991) Arch. Biochem. Biophys. 291, 176-186.
16. Ryan, R., Grimm, S. W., Kedzie, K. M., Halpert, J. R., and Philpot, R. M. (1993) Arch. Biochem. Biophys. 304, 454-463.
17. Gasser, R., Negishi, M., and Philpot, R. M. (1988) Mol. Pharmacol. 32, 22-30.
18. Grimm, S. W., Dyroff, M. C., Philpot, R. M., and Halpert, J. R. (1994) Mol. Pharmacol. 46, 1090-1099.
19. Gotoh, O. (1992) J. Biol. Chem. 267, 83-90.
20. Barnes, H. J., Arlotto, M. P., and Waterman, M. R. (1991) Proc. Natl. Acad. Sci. USA 88, 5597-5601.
21. John, G. H., Hasler, J. A., He, Y.-A., and Halpert, J. R. (1994) Arch. Biochem. Biophys. 314, 367-375.
22. Cullen, B. R. (1987) Methods Enzymol. 152, 684-704.
23. Stemmer, W. P., Morris, S. K., and Wilson, B. S. (1993) Biotechniques 14, 256-265.
24. Nash, T. (1953) Biochem. J. 55, 416-421.
25. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem. 193, 265-275.
26. Omura, T., and Sato, R. (1964) J. Biol. Chem. 239, 2379-2387.
27. Aoyama, T., Korzekwa, K., Nagata, K., Adesnik, M., Reiss, A., Lapenson, D. P., Gillette, J., Gelboin, H. V., Vaxman, D. J., and Gonzalez, F. J. (1989) J. Biol. Chem. 264, 21327-21333.
28. Hasemann, C. A., Kurumbail, R. G., Boddupalli, S. S., Peterson, J. A., and Deisenhofer, J. (1995) Structure 3, 41-62.
29. Poulos, T. L., Finzel, B. C., and Howard, A. (1987) J. Mol. Biol. 195, 687-700.