Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions

Methods

Volumn 32, Issue 4, 2004, Pages 349-362

  Fetchko, Michael ^a   Stagljar, Igor ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Complementation assay; Membrane proteins; Protein protein interactions; Protocols; Split ubiquitin; Split ubiquitin membrane yeast two hybrid; Yeast two hybrid

Indexed keywords

MEMBRANE PROTEIN; UBIQUITIN;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BIOLOGICAL MONITORING; BIOTECHNOLOGY; BIOTRANSFORMATION; COMPLEX FORMATION; CONTROLLED STUDY; DIMERIZATION; EXPRESSION VECTOR; GENETIC ANALYSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PROTEIN INTERACTION; REPRODUCIBILITY; SEQUENCE ANALYSIS; TWO HYBRID SYSTEM;

BETA-GALACTOSIDASE; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE LIBRARY; GENES, REPORTER; GENETIC VECTORS; HISTIDINE; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION FACTORS; TRANSFORMATION, GENETIC; TWO-HYBRID SYSTEM TECHNIQUES; UBIQUITIN;

EID: 1542285258     PISSN: 10462023     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymeth.2003.10.010     Document Type: Article

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