Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays

Structure

Volumn 10, Issue 6, 2002, Pages 763-772

  Reinders, Anke ^a   Schulze, Waltraud ^b   Thaminy, Safia ^c   Stagljar, Igor ^c,d   Frommer, Wolf B ^b   Ward, John M ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF TÜBINGEN   (Germany)

^c UNIVERSITY OF ZURICH   (Switzerland)

^d DUALSYSTEMS BIOTECH AG   (Switzerland)

Author keywords

Assembly; Dimer; Heteromeric interaction; Membrane protein; Structure; Sucrose transporter

Indexed keywords

MEMBRANE PROTEIN; PROTEIN SUT1; SUCROSE; UBIQUITIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL MEMBRANE; CELL MEMBRANE TRANSPORT; CHIMERA; MOLECULAR INTERACTION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SUGAR TRANSPORT;

INSERTION SEQUENCES;

EID: 0036284612     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(02)00773-6     Document Type: Article

References (51)

1. The dual function of sugar carriers: Transport and sugar sensing
2. SUT2, a putative sucrose sensor in sieve elements
3. Protein-protein interactions between sucrose transporters of different affinities co-localized in the same enucleate sieve element
4. A new subfamily of sucrose transporters, SUT4, with low-affinity/high-capacity localized in enucleate sieve elements of plants
5. +/sucrose cotransporter StSUT1
6. Potato sucrose transporter expression in minor veins indicates a role in phloem loading
7. Antisense repression of the sucrose transporter affects assimilate partitioning in transgenic potato plants
8. Companion cell-specific inhibition of the potato sucrose transporter SUT1
9. +-sucrose cotransporter NtSUT1 is essential for sugar export from tobacco leaves
10. The three-dimensional structure of aquaporin-1
11. + conduction and selectivity
12. Three-dimensional organization of a human water channel
13. Structure of MsbA from E. coli: A homolog of the multidrug resistance ATP binding cassette (ABC) transporters
14. The kamikaze approach to membrane transport
15. The human erythrocyte sugar transporter presents two sugar import sites
16. A novel genetic system to detect protein-protein interactions
17. Genome-wide protein interaction maps using two-hybrid systems
18. Large-scale analysis of the yeast proteome by multidimensional protein identification technology
19. Detecting interactions between membrane proteins in vivo using chimeras
20. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo
21. Evolution of transport proteins
22. Structural analysis of a plant sucrose carrier using monoclonal antibodies and bacteriophage lambda surface display
23. SUC1 and SUC2: Two sucrose transporters from Arabidopsis thaliana; Expression and characterization in baker's yeast and identification of the histidine-tagged protein
24. Sucrose transport in higher plants
25. Probing the molecular environment of membrane proteins in vivo
26. Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments
27. Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast
28. Sugar transporters from bacteria, parasites and mammals: Structure-activity relationships
29. Function of the cytosolic N-terminus of sucrose transporter AtSUT2 in substrate affinity
30. A conserved amino acid motif (R-X-G-R-R) in the Glut1 glucose transporter is an important determinant of membrane topology
31. Membrane topology of the ATP binding cassette transporter ABCR and its relationship to ABC1 and related ABCA transporters: Identification of N-linked glycosylation sites
32. The large cytoplasmic loop of the glucose transporter GLUT1 is an essential structural element for function
33. The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion
34. Expression of lactose permease in contiguous fragments as a probe for membrane spanning domains
35. Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
36. Glucose transporter function is controlled by transporter oligomeric structure. A single, intramolecular disulfide promotes GLUT1 tetramerization
37. Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1
38. Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli
39. Purification and functional characterization of the C-terminal half of the lactose permease of Escherichia coli
40. Model of the 3-D structure of the GLUT3 glucose transporter and molecular dynamics simulation of glucose transport
41. Mammalian Ras interacts directly with the serine/threonine kinase Raf
42. Improved method for high efficiency transformation of intact yeast cells
43. Split ubiquitin as a sensor of protein interactions in vivo
44. Dominant genetics using a yeast genomic library under the control of a strong inducible promoter
45. NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis
46. Macromolecular trafficking indicated by localization and turnover of sucrose transporters in enucleate sieve elements
47. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis
48. Vhmpt: A graphical viewer and editor for helical membrane protein topologies