Differential regulation and relocalization of the platelet P2Y receptors after activation: A way to avoid loss of hemostatic properties?

Molecular Pharmacology

Volumn 67, Issue 3, 2005, Pages 721-733

  Baurand, Anthony ^a   Eckly, Anita ^a   Hechler, Béatrice ^a   Kauffenstein, Gilles ^a   Galzi, Jean Luc ^b   Cazenave, Jean Pierre ^a   Léon, Catherine ^a   Gachet, Christian ^a  

^a INSERM   (France)

^b Cancerogenese Mutagenese Molec S   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; CALCIUM; CLATHRIN; CYCLIC AMP; GREEN FLUORESCENT PROTEIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; POLYCLONAL ANTIBODY; PURINE P2Y1 RECEPTOR; PURINE P2Y12 RECEPTOR;

ARTICLE; ASTROCYTOMA CELL; CALCIUM SIGNALING; CELL MEMBRANE; CONTROLLED STUDY; DESENSITIZATION; DRUG INHIBITION; ELECTRON MICROSCOPY; GENETIC TRANSFECTION; HEMOSTASIS; HUMAN; HUMAN CELL; INTERNALIZATION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; THROMBOCYTE ACTIVATION;

ADENOSINE DIPHOSPHATE; AMINO ACID SEQUENCE; ASTROCYTOMA; BLOOD PLATELETS; CELL LINE, TUMOR; CELL MEMBRANE; CYCLIC AMP; HEMOSTASIS; HUMANS; MEMBRANE PROTEINS; MICROSCOPY, CONFOCAL; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECEPTORS, PURINERGIC P2; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 14944348417     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.104.004846     Document Type: Article

References (40)

1. Ardlie NG, Perry DW, Packham MA, and Mustard JF (1971) Influence of apyrase on stability of suspensions of washed rabbit platelets. Proc Soc Exp Biol Med 136:1021-1023.
2. Barber AJ and Jamieson GA (1970) Isolation and characterization of plasma membranes from human blood platelets. J Biol Chem 245:6357-6365.
3. Baurand A, Eckly A, Bari N, Leon C, Hechler B, Cazenave JP, and Gachet C (2000) Desensitization of the platelet aggregation response to ADP: differential down-regulation of the P2Y1 and P2cyc receptors. Thromb Haemost 84:484-491.
4. Cazenave JP, Hemmendinger S, Beretz A, Sutter-Bay A, and Launay J (1983) L'agrégation plaquettaire: outil d'investigation clinique et d'étude pharmacologique. Méthodologie. Ann Biol Clin 41:167-179.
5. Conley PB and Delaney SM (2003) Scientific and therapeutic insights into the role of the platelet P2Y12 receptor in thrombosis. Curr Opin Hematol 10:333-338.
6. Eckly A, Gendrault JL, Hechler B, Cazenave JP, and Gachet C (2001) Differential involvement of the P2Y1 and P2YT receptors in the morphological changes of platelet aggregation. Thromb Haemost 85:694-701.
7. Enjyoji K, Sevigny J, Lin Y, Frenette PS, Christie PD, Esch JS, 2nd, Imai M, Edelberg JM, Rayburn H, Lech M, et al. (1999) Targeted disruption of cd39/ATP diphosphohydrolase results in disordered hemostasis and thromboregulation. Nat Med 5:1010-1017.
8. Fabre JE, Nguyen M, Latour A, Keifer JA, Audoly LP, Coffman TM, and Koller BH (1999) Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1-deficient mice. Nat Med 5:1199-1202.
9. 2+ signaling in astrocytes. J Neurosci 23:4437-4444.
10. Fijnheer R, Boomgaard MN, van den Eertwegh AJ, Homburg CH, Gouwerok CW, Veldman HA, Roos D, and de Korte D (1992) Stored platelets release nucleotides as inhibitors of platelet function. Thromb Haemost 68:595-599.
11. Foster CJ, Prosser DM, Agans JM, Zhai Y, Smith MD, Lachowicz JE, Zhang FL, Gustafson E, Monsma FJ Jr, Wiekowski MT, et al. (2001) Molecular identification and characterization of the platelet ADP receptor targeted by thienopyridine antithrombotic drugs. J Clin Investig 107:1591-1598.
12. Gachet C (2001) ADP receptors of platelets and their inhibition. Thromb Haemost 86:222-232.
13. Gachet C, Cattaneo M, Ohlmann P, Hechler B, Lecchi A, Chevalier J, Cassel D, Mannucci PM, and Cazenave JP (1995) Purinoceptors on blood platelets: further pharmacological and clinical evidence to suggest the presence of two ADP receptors. Br J Haematol 91:434-444.
14. Gachet C, Hanau D, Spehner D, Brisson C, Garaud JC, Schmitt DA, Ohlmann P, and Cazenave JP (1993) Alpha IIb beta 3 Integrin dissociation induced by EDTA results in morphological changes of the platelet surface-connected canalicular system with differential location of the two separate subunits. J Cell Biol 120:1021-1030.
15. Hall RA and Lefkowitz RJ (2002) Regulation of G protein-coupled receptor signaling by scaffold proteins. Circ Res 91:672-680.
16. Hammes SR, Shapiro MJ, and Coughlin SR (1999) Shutoff and agonist-triggered internalization of protease-activated receptor 1 can be separated by mutation of putative phosphorylation sites in the cytoplasmic tail. Biochemistry 38:9308-9316.
17. Hardy AR, Jones ML, Mundell SJ, and Poole AW (2004) Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human platelets. Blood 104:1745-1752.
18. Hechler B, Eckly A, Ohlmann P, Cazenave JP, and Gachet C (1998a) The P2Y1 receptor, necessary but not sufficient to support full ADP-induced platelet aggregation, is not the target of the drug clopidogrel. Br J Haematol 103:858-866.
19. Hechler B, Leon C, Vial C, Vigne P, Frelin C, Cazenave JP, and Gachet C (1998b) The P2Y1 receptor is necessary for adenosine 5′-diphosphate- induced platelet aggregation. Blood 92:152-159.
20. Herbert JM and Savi P (2003) P2Y12, a new platelet ADP receptor, target of clopidogrel. Semin Vasc Med 3:113-122.
21. Hollopeter GJH, Vincent D, Li G, England L, Ramakrishan V, Yang RB, Nurden P, Nurden A, Julius D, and Conley PB (2001) Identification of the platelet ADP receptor targeted by antithrombotic drugs. Nature (Lond) 409:202-207.
22. Holme S and Holmsen H (1975) ADP-induced refractory state of platelets in vitro. I. Methodological studies on aggregation in platelet rich plasma. Scand J Haematol 15:96-103.
23. Humphries RG (2000) Pharmacology of AR-C69931MX and related compounds: from pharmacological tools to clinical trials. Haematologica 85:66-72.
24. Kauffenstein G, Bergmeier W, Eckly A, Ohlmann P, Leon C, Cazenave JP, Nieswandt B, and Gachet C (2001) The P2Y(12) receptor induces platelet aggregation through weak activation of the alpha(IIb)beta(3) integrin-a phosphoinositide 3-kinase-dependent mechanism. FEBS Lett 505:281-290.
25. 12 receptor. J Thromb Haemost 2:1980-1988.
26. Kawabata A, Saifeddine M, Al-Ani B, Leblond L, and Hollenberg MD (1999) Evaluation of proteinase-activated receptor-1 (PAR1) agonists and antagonists using a cultured cell receptor desensitization assay: activation of PAR2 by PAR1-targeted ligands. J Pharmacol Exp Ther 288:358-370.
27. Kim S, Jin J, and Kunapuli SP (2004) Akt activation in platelets depends on Gi signaling pathways. J Biol Chem 279:4186-4195.
28. Leon C, Hechler B, Freund M, Eckly A, Vial C, Ohlmann P, Dierich A, LeMeur M, Cazenave JP, and Gachet C (1999) Defective platelet aggregation and increased resistance to thrombosis in purinergic P2Y(1) receptor-null mice. J Clin Investig 104:1731-1737.
29. Marcus AJ, Broekman MJ, Drosopoulos JH, Islam N, Alyonycheva TN, Safier LB, Hajjar KA, Posnett DN, Schoenborn MA, Schooley KA, et al. (1997) The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39. J Clin Investig 99:1351-1360.
30. Moro O, Lameh J, Hogger P, and Sadee W (1993) Hydrophobic amino acid in the i2 loop plays a key role in receptor-G protein coupling. J Biol Chem 268:22273-22276.
31. O'Brien JR (1965) Effects of adenosine diphosphate and adrenaline on mean platelet shape. Nature (Lond) 207:306-307.
32. O'Brien JR, Etherington M, and Jamieson S (1971) Refractory state of platelet aggregation with major operations. Lancet 2:741-743.
33. Okwu AK, Ullian ME, and Halushka PV (1992) Homologous desensitization of human platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262:238-245.
34. Poole AW, Heath MF, and Evans RJ (1993) ADP induces desensitisation of equine platelet aggregation responses: studies using ADP beta S, a stable analogue of ADP. Res Vet Sci 54:235-243.
35. Robinson JM, Takizawa T, and Vandre DD (2000) Applications of gold cluster compounds in immunocytochemistry and correlative microscopy: comparison with colloidal gold. J Microsc 199:163-179.
36. Robson SC, Kaczmarek E, Siegel JB, Candinas D, Koziak K, Millan M, Hancock WW, and Bach FH (1997) Loss of ATP diphosphohydrolase activity with endothelial cell activation. J Exp Med 185:153-163.
37. Roevens P and de Chaffoy de Courcelles D (1995) Desensitization and resensitization of human platelets to 5-hydroxytryptamine at the level of signal transduction. Biochem J 307:775-782.
38. Schwarz UR, Geiger J, Walter U, and Eigenthaler M (1999) Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets-definition and detection of ticlopidine/clopidogrel effects. Thromb Haemost 82:1145-1152.
39. Spaet TH and Lejnieks I (1966) Studies on the mechanism whereby platelets are clumped by adenosine diphosphate. Thromb Diath Haemorrh 15:36-51.
40. Trumel C, Payrastre B, Plantavid M, Hechler B, Viala C, Presek P, Martinson EA, Cazenave JP, Chap H, and Gachet C (1999) A key role of adenosine diphosphate in the irreversible platelet aggregation induced by the PAR1-activating peptide through the late activation of phosphoinositide 3-kinase. Blood 94:4156-4165.