Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for quantitation and molecular stability assessment of insulin entrapped within PLGA nanoparticles

Journal of Pharmaceutical Sciences

Volumn 94, Issue 3, 2005, Pages 688-694

  Bilati, Ugo ^a   Pasquarello, Carla ^b   Corthals, Carry L ^b   Hochstrasser, Denis F ^a,b   Allémann, Eric ^a,c   Doelker, Eric ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b GENEVA UNIVERSITY HOSPITALS   (Switzerland)

^c Bracco Suisse SA   (Switzerland)

Author keywords

Drug loading; Entrapment efficiency; Insulin; MALDI TOF; Mass spectrometry; Nanoparticles; Peptides; Poly(lactic glycolic) acid (PLGA); Protein stability; Proteins; Quantitation

Indexed keywords

DIMER; INSULIN; NANOPARTICLE; POLYGLACTIN;

ARTICLE; CONCENTRATION (PARAMETERS); DRUG STABILITY; EXTRACTION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ANALYSIS; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET SPECTROSCOPY;

EID: 14844301487     PISSN: 00223549     EISSN: None     Source Type: Journal    
DOI: 10.1002/jps.20266     Document Type: Article

References (25)

1. Gupta RK, Chang AC, Griffin P, Rivera R, Guo YY, Siber GR. 1997. Determination of protein loading in biodegradable polymer microspheres containing tetanus toxoid. Vaccine 15:672-678.
2. Fu K, Griebenow K, Hsieh L, Klibanov AM, Langer R. 1999. FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres. J Controlled Release 58:357-366.
3. Yang TH, Dong A, Meyer J, Johnson OL, Cleland JL, Carpenter JF. 1999. Use of infrared spectroscopy to assess secondary structure of human growth hormone within biodegradable microspheres. J Pharm Sci 88:161-165.
4. Van de Weert M, Van't Hof R, Van der Weerd J, Heeren RM, Posthuma G, Hennink WE, Crommelin DJ. 2000. Lysozyme distribution and conformation in a biodegradable polymer matrix as determined by FTIR techniques. J Controlled Release 68:31-40.
5. Sutton C. 2003. MALDI: The key to MS protein characterisation. New Drugs 5:24-25.
6. Joshi AB, Kirsch LE. 2002. The relative rates of glutamine and asparagine deamidation in glucagon fragment 22-29 under acidic conditions. J Pharm Sci 91:2332-2345.
7. Park ZY, Russell DH. 2001. Identification of individual proteins in complex protein mixtures by high-resolution, high-mass-accuracy MALDI TOF-mass spectrometry analysis of in-solution thermal denaturation/enzymatic digestion. Anal Chem 73:2558-2564.
8. Leize EM, Leize EJ, Leize MC, Voegel JC, Van Dorsselaer A. 1999. Matrix-assisted laser desorption ionization mass spectrometry: A new tool for probing interactions between proteins and metal surfaces. Anal Biochem 272:19-25.
9. Matsumoto H, Kahn ES, Komori N. 1998. Non-radioactive phosphopeptide assay by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: Application to calcium/calmodulin-dependent protein kinase II. Anal Biochem 260:188-194.
10. Pitt JJ, Gorman JJ. 1997. Oligosaccharide characterization and quantitation using 1-phenyl-3-methyl-5-pyrazolone derivatization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem 248:63-75.
11. Bucknall M, Fung KYC, Duncan MW. 2002. Practical quantitative biomedical applications of MALDI-TOF mass spectrometry. J Am Soc Mass Spectrom 13:1015-1027.
12. Stocklin R, Vu L, Vadas L, Cerini F, Kippen AD, Offord RE, Rose K. 1997. A stable isotope dilution assay for the in vivo determination of insulin levels in humans by mass spectrometry. Diabetes 46:44-50.
13. Yang HH, Li XC, Amft M, Grotemeyer J. 1998. Protein conformational changes determined by matrix-assisted laser desorption mass spectrometry. Anal Biochem 258:118-126.
14. Kostanski JW, Thanoo BC, DeLuca PP. 2000. Preparation, characterization, and in vitro evaluation of 1- and 4-month controlled release Orntide PLA and PLGA microspheres. Pharm Dev Technol 5:585-596.
15. Diwan M, Park TG. 2001. Pegylation enhances protein stability during encapsulation in PLGA microspheres. J Controlled Release 73:233-244.
16. Zydiwicz N, Chaumont P, Soto-Portas ML. 2001. Formation of aqueous core polyamide microcapsules obtained via interfacial polycondensation: Optimization of the membrane formation through pH control. J Membr Sci 189:41-58.
17. Na DH, Lee SS, An HM, Kim HJ, Lee KC. 2001. MALDI-TOF mass spectrometry for direct determination of the actual drug content in PLGA microspheres. Proc Int Symp Controlled Release Bioact Mater 28:956-957.
18. Na DH, De Luca PP, Lee KC. 2004. Direct determination of the peptide content in microspheres by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem 76:2669-2673.
19. Na DH, Youn YS, Lee SD, Son MW, Kim WB, De Luca PP, Lee KC. 2003. Monitoring of peptide acylation inside degrading PLGA microspheres by capillary electrophoresis and MALDI-TOF mass spectometry. J Controlled Release 92:291-299.
20. Bilati U, Allémann E, Doelker E. 2003. Sonication parameters for the preparation of biodegradable nanocapsules of controlled size by the double emulsion method. Pharm Dev Technol 8:1-9.
21. Darrington RT, Anderson BD. 1995. Evidence for a common intermediate in insulin deamidation and covalent dimer formation: Effects of pH and aniline trapping in dilute acidic solutions. J Pharm Sci 84:275-282.
22. Wilkinson WR, Gusev AI, Proctor A, Houalla M, Hercules DM. 1997. Selection of internal standards for quantitative analysis by matrix-assisted laser desorption-ionization (MALDI) time-of-flight mass spectrometry. Fresenius' J Anal Chem 357:241-248.
23. Oliva A, Farina J, Llabres M. 1997. Analysis of peptides and proteins: Evaluation of purity, stability, and structural characterization of insulin. Drug Dev Ind Pharm 23:915-927.
24. Solouki T, Russel DH. 1992. Laser desorption studies of high mass biomolecules in Fourier-transform ion cyclotron resonance mass spectrometry. Proc Natl Acad Sci USA 89:5701-5704.
25. Spengler B, Kirsch D, Kaufmann R, Jaeger E. 1992. Peptide sequencing by matrix-assisted laser-desorption mass spectrometry. Rapid Commun Mass Spectrom 6:105-108.