Performing functional studies of Xenopus laevis intermediate filament proteins through injection of macromolecules into early embryos

Methods in Cell Biology

Volumn 2004, Issue 78, 2004, Pages 673-701

  Gervasi, Christine ^a   Szaro, Ben G ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ANTISENSE OLIGONUCLEOTIDE; INTERMEDIATE FILAMENT PROTEIN; NERVE PROTEIN; RNA;

ANIMAL; ANIMAL MODEL; ARTICLE; DRUG ANTAGONISM; GENETICS; INSTRUMENTATION; METABOLISM; MICROINJECTION; PLASMID; PRENATAL DEVELOPMENT; XENOPUS LAEVIS;

ANIMALS; ANTIBODIES; INTERMEDIATE FILAMENT PROTEINS; MICROINJECTIONS; MODELS, ANIMAL; NERVE TISSUE PROTEINS; OLIGONUCLEOTIDES, ANTISENSE; PLASMIDS; RNA; XENOPUS LAEVIS;

EID: 14244268836     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(04)78023-1     Document Type: Review

References (119)

1. Amaya, E., and Kroll, K. L. (1999). A method for generating transgenic frog embryos. Methods Mol. Biol. 97, 393-414.
2. Asch, W. S., Leake, D., Canger, A. K., Passini, M. A., Argenton, F., and Schechter, N. (1998). Cloning of zebrafish neurofilament cDNAs for plasticin and gefiltin: Increased mRNA expression in ganglion cells after optic nerve injury. J. Neurochem. 71, 20-32.
3. Beaulieu, J.-M., Jacomy, H., and Julien, J.-P. (2000). Formation of intermediate filament protein aggregates with disparate effects in two transgenic mouse models lacking the neurofilament light subunit. J. Neurosci. 20, 5321-5328.
4. Beaulieu, J.-M., Robertson, J., and Julien, J.-P. (1999). Interactions between peripherin and neurofilaments in cultured cells: Disruption of peripherin assembly by the NF-M and NF-H subunits. Biochem. Cell Biol. 77, 41-45.
5. Bennett, G. S., Hollander, B. A., and Laskowska, D. (1988). Expression and phosphorylation of the mid-sized neurofilament protein NF-M during chick spinal cord neurogenesis. J. Neurosci. Res. 21, 376-390.
6. Boyne, L. J., Fischer, I., and Shea, T. B. (1996). Role of vimentin in early stages of neuritogenesis in cultured hippocampal neurons. Int. J. Dev. Neurosci. 14, 739-748.
7. Canger, A. K., Passini, M. A., Asch, W. S., Leake, D., Zafonte, B. T., Glasgow, E., and Schechter, N. (1998). Restricted expression of the neuronal intermediate filament protein plasticin during zebrafish development. J. Comp. Neurol. 399, 561-572.
8. Carden, M. J., Trojanowski, J. Q., Schlaepfer, W. W., and Lee, V. M. Y. (1987). Two-stage expression of neurofilament polypeptides during rat neurogenesis with early establishment of adult phosphorylation patterns. J. Neurosci. 7, 3489-3504.
9. Carl, T. F., Dufton, C., Hanken, J., and Klymkowsky, M. W. (1999). Inhibition of neural crest migration in Xenopus using antisense slug RNA. Dev. Biol. 213, 101-115.
10. Gary, R. B., and Klymkowsky, M. W. (1995). Disruption of intermediate filament organization leads to structural defects at the intersomite junction in Xenopus myotomal muscle. Development 121, 1041-1052.
11. Charnas, L. R., Szaro, B. G., and Gainer, H. (1992). Identification and developmental expression of a novel low molecular weight neuronal intermediate filament protein in Xenopus laevis. J. Neurosci. 12, 3010-3024.
12. Chou, Y.-H., Helfand, B. T., and Goldman, R. D. (2001). New horizons in cytoskeletal dynamics: Transport of intermediate filaments along microtubule tracks. Curr. Opin. Cell Biol. 13, 106-109.
13. Christian, J. L., Edelstein, N. G., and Moon, R. T. (1990). Overexpression of wild-type and dominant negative mutant vimentin subunits in developing Xenopus embryos. The New Biologist. 2, 700-711.
14. Chu, D. T. W., and Klymkowsky, M. W. (1987). Experimental analysis of cytoskeletal function in early Xenopus laevis embryos. First Intntl. Symp. Cytoskel. Der. 8, 140-142.
15. Cochard, P., and Paulin, D. (1984). Initial expression of neurofilaments and vimentin in the central and peripheral nervous system of the mouse embryo in vivo. J. Neurosci. 4, 2080-2094.
16. Colucci-Guyon, E., Portier, M.-M., Dunia, I., Paulin, D., Pournin, S., and Babinet, C. (1994). Mice lacking vimentin develop and reproduce without an obvious phenotype. Cell 79, 679-694.
17. de Waegh, S., Lee, V. M. Y., and Brady, S. T. (1992). Local modulation of neurofilament phosphorylation, axonal caliber, and slow axonal transport by myelinating Schwann cells. Cell 68, 451-463.
18. Dent, J. A., Polson, A. G., and Klymkowsky, M. W. (1989). A wholemount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. Development 105, 61-74.
19. Eckes, B., Dogic, D., Colucci-Guyon, E., Wang, N., Maniotis, A., Ingber, D., Merckling, A., Langa, F., Aumailley, M., Delouvèe, A., Koteliansky, V., Babinet, C., and Krieg, T. (1998). Impaired mechanical stability, migration and contractile capacity in vimentin deficient fibroblasts. J. Cell Sci. 111, 1897-1907.
20. Elder, G. A., Friedrich, V. L., Jr., Bosco, P., Kang, C., Giourov, A., Tu, P.-H., Lee, V. M. Y., and Lazzarini, R. A. (1998a). Absence of the mid-sized neurofilament subunit decreases axonal calibers, levels of light neurofilament (NF-L) and neurofilament content. J. Cell Biol. 141, 727-739.
21. Elder, G. A., Friedrich, V. L., Jr., Kang, C., Bosco, P., Gourov, A., Tu, P.-H., Zhang, B., Lee, V. M. Y., and Lazzarini, R. A. (1998b). Requirement of heavy neurofilament subunit in the development of axons with large calibers. J. Cell Biol. 143, 195-205.
22. Eyer, J., and Peterson, A. (1994). Neurofilament-deficient axons and perikaryal aggregates in viable transgenic mice expressing a neurofilament-beta-galacotsidase fusion protein. Neuron 12, 389-405.
23. Faigle, W., Colucci-Guyon, E., Louvard, D., Amigorena, S., and Galli, T. (2000). Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery. Mol. Biol. Cell. 11, 3485-3494.
24. Fliegner, K. H., Kaplan, M. P., Wood, T. L., Pintar, J. E., and Liem, R. K. H. (1994). Expression of the gene for the neuronal intermediate filament protein alpha-internexin coincides with the onset of neuronal differentiation in the developing rat nervous system. J. Comp. Neurol. 342, 161-173.
25. Fuchs, E., and Cleveland, D. W. (1998). A structural scaffolding of intermediate filaments in health and disease. Science 279, 514-519.
26. Garcia, M. L., Lobsiger, C. S., Shah, S. B., Deerinck, T. J., Crum, J., Young, D., Ward, C. M., Crawford, T. O., Gotow, T., Uchiyama, Y., Ellisman, M. H., Calcutt, N. A., and Cleveland, D. W. (2003). NF-M is an essential target for the myelin-directed "outside-in" signaling cascade that mediates radial axonal growth J. Cell Biol. 163, 1011-1020.
27. Gervasi, C., Stewart, C.-B., and Szaro, B. G. (2000). Xenopus laevis peripherin (XIF3) is expressed in radial glia and proliferating neural epithelial cells as well as in neurons. J. Comp. Neurol. 423, 512-531.
28. Giasson, B. I., and Mushynski, W. E. (1998). Intermediate filament disassembly in cultured dorsal root ganglion neurons is associated with amino-terminal head domain phosphorylation of specific subunits. J. Neurochem. 70, 1869-1875.
29. Giebelhaus, D. H., Eib, D. W., and Moon, R. T. (1988). Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus. Cell 53, 601-615.
30. Gimlich, R. L. (1991). Fluorescent dextran clonal markers. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 285-298. Academic Press, San Diego.
31. Gimlich, R. L., and Gerhart, J. C. (1984). Early cellular interactions promote embryonic axis formation in Xenopus laevis. Dev. Biol. 104, 117-130.
32. Glasgow, E., Druger, R. K., Fuchs, C., Lane, W. S., and Schechter, N. (1994). Molecular cloning of gefiltin (ON1): Serial expression of two new neurofilament mRNAs during optic nerve regeneration. EMBO J. 13, 297-305.
33. Goldman, R. D., Chao, Y.-H., Prahlad, V., and Yoon, M. (1999). Intermediate filaments: Dynamic processes regulating their assembly, motility, and interactions with other cytoskeletal systems. FASEB J. 13, S261-S265.
34. Goldman, R. D., Khuon, S., Chou, Y. H., Opal, P., and Steinert, P. M. (1996). The function of intermediate filaments in cell shape and cytoskeletal integrity. J. Cell Biol. 134, 971-983.
35. Goldstein, M. E., Cooper, H. S., Bruce, J., Carden, M. J., Lee, V. M. Y., and Schlaepfer, W. W. (1987). Phosphorylation of neurofilament proteins and chromatolysis following transection of rat sciatic nerve. J. Neurosci. 7, 1586-1594.
36. Goldstein, M. E., Weiss, S. R., Lazzarini, R. A., Shneidman, P. S., Lees, J. F., and Schlaepfer, W. W. (1988). mRNA levels of all three neurofilament proteins decline following nerve transection. Mol. Brain Res. 3, 287-292.
37. Goldstone, K., and Sharpe, C. R. (1998). The expression of Xif3 in undifferentiated anterior neuroectoderm, but not in primary neurons, is induced by the neuralizing agent noggin. Int. J. Dev. Biol. 42, 757-762.
38. Gurdon, J. B., (1996). In "Introductory comments: Xenopus as a laboratory organism" (R. C. Tinsley and H. R. Kobel, eds.), pp. 3-6. Oxford University Press, Oxford.
39. Harland, R. M. (1991). In situ hybridization: An improved whole mount method for Xenopus embryos. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 685-696. Academic Press, San Diego.
40. Heasman, J., Holwill, S., and Wylie, C. C. (1991). Fertilization of cultured Xenopus oocytes and use in studies of maternally inherited molecules. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 214-231. Academic Press, San Diego.
41. Hesse, M., Magin, T. M., and Weber, K. (2001). Genes for intermediate filament proteins and the draft sequence of the human genome: Novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18. J. Cell Sci. 114, 2569-2575.
42. Hirose, G., and Jacobson, M. (1979). Clonal organization of the central nervous system of the frog: I. Clones stemming from individual blastomeres of the 16-cell and earlier stages. Develop. Biol. 71, 191-202.
43. Hoffman, P. N., Cleveland, D. W., Griffin, J. W., Landes, P. W., Cowan, N. J., and Price, D. L. (1987). Neurofilament gene expression: A major determinant of axonal caliber. Proc. Natl. Acad. Sci. USA 84, 3472-3476.
44. p) in Xenopus laevis. J. Embryol. Exp. Morphol. 34, 253-264.
45. Inagaki, M., Matsuoka, Y., Tsujimura, K., Ando, S., Tokui, T., Takahashi, T., and Inagaki, N. (1996). Dynamic property of intermediate filaments: Regulation by phosphorylation. BioEssays 18, 481-487.
46. Jacobson, M., and Hirose, G. (1978). Origin of the retina from both sides of the embyonic brain: A contribution to the problem of crossing at the optic chiasma. Science 202, 637-639.
47. Jacomy, H., Zhu, Q., Beaulieu, J.-M., and Julien, J.-P. (1999). Disruption of Type IV intermediate filament network in mice lacking the neurofilament medium and heavy subunits. J. Neurochem. 73, 972-984.
48. Jiang, T. Q., Pickett, J., and Oblinger, M. M. (1994). Comparison of changes in Beta-tubulin and NF gene expression in rat DRG neurons under regeneration-permissive and regeneration-prohibitive conditions. Brain Res. 637, 233-241.
49. Jiang, X. M., Zhao, J. X., Ohnishi, A., Itakura, C., Mizutani, M., Yamamoto, T., Murai, Y., and Ikeda, M. (1996). Regeneration of myelinated fiber after crush injury is retarded in sciatic nerves of mutant Japanese quails deficient in neurofilaments. Acta Neuropathol. (Berl.). 92, 467-472.
50. Julien, J. P. (1999). Neurofilament functions in health and disease. Curr. Opin. Cell Biol. 9, 554-560.
51. Karabinos, A., Schmidt, H., Harborth, J., and Weber, K. (2001). Essential roles for four cytoplasmic intermediate filament proteins in Caenorhabditis elegans development. Proc. Natl. Acad. Sci. USA 98, 7863-7868.
52. Kay, B. K., and Peng, H. B. (1991). "Methods in cell biology, Vol. 36, Xenopus laevis: Practical uses in cell and molecular biology." Academic Press, San Diego.
53. Keller, R. (1991). Early embryonic development in Xenopus laevis. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 62-116. Academic Press, San Diego.
54. Kim, O.-K., Ariano, M. A., Lazzarini, R. A., Levine, M. S., and Sibley, D. R. (2002). Neurofilament-M interacts with the D1 dopamine receptor to regulate cell surface expression and desensitization. J. Neurosci. 22, 5920-5930.
55. Kintner, C. (1988). Effects of altered expression of the neural cell adhesion molecule, N-CAM, on early neural development in Xenopus embryos. Neuron 1, 545-555.
56. Klymkowsky, M. W., and Hanken, J. (1991). Whole-mount staining of Xenopus and other vertebrates. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 420-443. Academic Press, San Diego.
57. Klymkowsky, M. W., Miller, R. H., and Lane, E. B. (1983). Morphology, behavior, and interaction of cultured epithelial cells after the antibody-induced disruption of keratin filament organization. J. Cell Biol. 96, 494-509.
58. Klymkowsky, M. W., Shook, D. R., and Maynell, L. A. (1992). Evidence that the deep keratin filament systems of the Xenopus embryo act to ensure normal gastrulation. Proc. Natl. Acad. Sci. USA 89, 8736-8740.
59. Lander, E. S., Linton, L. M., and others (2001). Initial sequencing and analysis of the human genome. Nature 409, 860-921.
60. Landmesser, L., and Swain, S. (1992). Temporal and spatial modulation of a cytoskeletal antigen during peripheral axonal pathfinding. Neuron 8, 291-305.
61. Lariviere, R. C., Nguyen, M. D., Ribera-da-Silva, A., and Julien, J.-P. (2002). Reduced number of unmyelinated sensory axons in peripherin null mice. J. Neurochem. 81, 525-532.
62. Leake, D., Asch, W. S., Canger, A. K., and Schechter, N. (1999). Gefiltin in zebrafish embryos: Sequential gene expression of two neurofilament proteins in retinal ganglion cells. Diff. 65, 181-189.
63. Levavasseur, F., Zhu, Q., and Julien, J.-P. (1999). No requirement of alpha-internexin for nervous system development and for radial growth of axons. Mol. Brain Res. 69, 104-112.
64. Lin, W., and Szaro, B. G. (1994). Maturation of neurites in mixed cultures of spinal cord neurons and muscle cells from Xenopus laevis embryos followed with antibodies to neurofilament proteins. J. Neurobiol. 25, 1235-1248.
65. Lin, W., and Szaro, B. G. (1995). Neurofilaments help maintain normal morphologies and support elongation of neurites in Xenopus laevis cultured embryonic spinal cord neurons. J. Neurosci. 15, 8331-8344.
66. Lin, W., and Szaro, B. G. (1996). Effects of intermediate filament disruption on the early development of the peripheral nervous system of Xenopus laevis. Dev. Biol. 179, 197-211.
67. Liuzzi, F. J., and Tedeschi, B. (1992). Axo-glial interactions at the dorsal root transitional zone regulate neurofilament protein synthesis in axotomized sensory neurons. J. Neurosci. 12, 4783-4792.
68. Lombardo, A., and Slack, J. M. W. (1997). Inhibition of eFGF expression in Xenopus embryos by antisense mRNA. Dev. Dyn. 208, 162-169.
69. Mikucki, S. A., and Oblinger, M. M. (1991). Corticospinal neurons exhibit a novel pattern of cytoskeletal gene expression after injury. J. Neurosci. Res. 30, 213-225.
70. Nakano, H., Amemiya, S., Shiokawa, K., and Taira, M. (2001). RNA interference for the organizer-specific gene Xlim-1 in Xenopus embryos. Biochem. Biophys. Res. Commun. 274, 434-439.
71. Nasevicius, A., and Ekker, S. C. (2000). Effective targeted gene 'knockdown' in zebrafish. Nature Genetics. 26, 129-130.
72. Nichols, A., Rungger-Brändle, E., Muster, L., and Rungger, D. (1995). Inhibition of XhoxIA gene expression in Xenopus embryos by antisense RNA produced from an expression vector read by RNA polymerase III. Mech. Dev. 52, 37-49.
73. Nieuwkoop, P. D., and Faber, J. (1994). "Normal table of Xenopus laevis (Daudin)." Garland Publishing, New York.
74. Nutt, S. L., Bronchain, O. J., Hartley, K. O., and Amaya, E. (2001). Comparison of morpholino based translational inhibition during the development of Xenopus laevis and Xenopus tropicalis. Genesis 30, 110-113.
75. O'Keefe, H. P., Melton, D. A., Ferreiro, B., and Kintner, C. (1991). In situ hybridization. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 443-695. Academic Press, San Diego.
76. Pachter, J. S., and Liem, R. K. H. (1984). The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve. Dev. Biol. 103, 200-210.
77. Peng, H. B., Baker, L. P., and Chen, Q. (1991). Tissue culture of Xenopus neurons and muscle cells as a model for studying synaptic induction. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 511-526. Academic Press, San Diego.
78. Rao, M. V., Campbell, J., Yuan, A., Kumar, A., Gotow, T., Uchiyama, Y., and Nixon, R. A. (2003). The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. J. Cell Biol. 163, 1021-1031.
79. Rao, M. V., Garica, M. L., Miyazaki, Y., Gotow, T., Yuan, A., Mattina, S., Ward, C. M., Calcutt, N. A., Uchiyama, Y., Nixon, R. A., and Cleveland, D. W. (2002). Gene replacement in mice reveals that the heavily phosphorylated tail of neurofilament heavy subunit does not affect axonal caliber or the transit of cargoes in slow axonal transport. J. Cell Biol. 158, 681-693.
80. Rao, M. V., Houseweart, M. K., Williamson, T. L., Crawford, T. O., and Folmer, J. (1998). Neurofilament-dependent radial growth of motor axons and axonal organization of neurofilaments does not require the neurofilament heavy subunit (NF-H) or its phosphorylation. J. Cell Biol. 143, 171-181.
81. Rebagliati, M. R., and Melton, D. A. (1987). Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity. Cell 48, 599-605.
82. Retaux, S., McNeill, L., and Harris, W. A. (1996). Engrailed, retinotectal targeting, and axonal patterning in the midbrain during Xenopus development: An antisense study. Neuron 16, 63-75.
83. Roosa, J. R., Gervasi, C., and Szaro, B. G. (2000). Structure, biological activity of the upstream regulatory sequence, and conserved domains of a middle molecular mass neurofilament gene of Xenopus laevis. Mol Brain Res. 82, 35-51.
84. Sánchez, I., Hassinger, L., Paskevich, P. A., Shine, H. D., and Nixon, R. A. (1996). Oligodendroglia regulate the regional expansion of axon caliber and local accumulation of neurofilaments during development independently of myelin formation. J. Neurosci. 16, 5095-5105.
85. Sakaguchi, T., Okada, M., Kitamura, T., and Kawasaki, K. (1993). Reduced diameter and conduction velocity of myelinated fibers in the sciatic nerve of a neurofilament-deficient mutant quail. Neurosci. Lett. 153, 65-68.
86. Schwartz, M. L., Shneidman, P. S., Bruce, J., and Schlaepfer, W. W. (1990). Axonal dependency of the postnatal upregulation in neurofilament expression. J. Neurosci. Res. 27, 193-201.
87. Schwartz, M. L., Shneidman, P. S., Bruce, J., and Schlaepfer, W. W. (1994). Stabilization of neurofilament transcripts during postnatal development. Mol. Brain Res. 27, 215-220.
88. Shain, D. H., and Zuber, M. X. (1996). Sodium dodecyl sulfate (SDS)-based whole-mount in situ hybridization of Xenopus laevis embryos. J. Biochem. Biophys. Meth. 31, 185-188.
89. Shaw, G., Banker, G. A., and Weber, K. (1985). An immunofluorescence study of neurofilament protein expression by developing hippocampal neurons in tissue culture. Eur. J. Cell Biol. 39, 205-216.
90. Shaw, G., and Weber, K. (1982). Differential expression of neurofilament triplet proteins in brain development. Nature 298, 277-279.
91. Shea, T. B., Beerman, M. L., and Fischer, I. (1993). Transient requirement for vimentin in neuritogenesis: Intracellular delivery of anti-vimentin antibodies and antisense oligonucleotides inhibit neurite initiation, but not elongation of exiting neurites in neuroblastoma. J. Neurosci. Res. 36, 66-76.
92. Steinbeisser, H., Fainsod, A., Niehrs, C., Sasai, Y., and De Robertis, E. M. (1995). The role of gsc and BMP-4 in dorsal-ventral patterning of the marginal zone in Xenopus: A loss-of-function study using antisense RNA. EMBO J. 14, 5230-5243.
93. Sumikawa, K., and Miledi, R. (1988). Repression of nicotinic acetylcholine receptor expression by antisense RNAs and an oligonucleotide. Proc. Natl. Acad. Sci. USA 85, 1302-1306.
94. Szaro, B. G., and Gainer, H. (1988). Identities, antigenic determinants, and topographic distributions of neurofilament proteins in the nervous systems of adult frogs and tadpoles of Xenopus laevis. J. Comp. Neurol. 273, 344-358.
95. Szaro, B. G., Grant, P., Lee, V. M. Y., and Gainer, H. (1991). Inhibition of axonal development after injection of neurofilament antibodies into a Xenopus laevis embryo. J. Comp. Neurol. 308, 576-585.
96. Szaro, B. G., Lee, V. M. Y., and Gainer, H. (1989). Spatial and temporal expression of phosphorylated and non-phosphorylated forms of neurofilament proteins in the developing nervous system of Xenopus laevis. Dev. Brain Res. 48, 87-103.
97. Tabti, N., and Poo, M.-M. (1991). Culturing spinal neurons and muscle cells from Xenopus embryos. In (G. Banker and K. Goslin, eds.), pp. 137-154. MIT Press, Cambridge.
98. Tapscott, S. J., Bennett, G. S., Toyama, Y., Kleinbart, F., and Holtzer, H. (1981). Intermediate filament proteins in the developing chick spinal cord. Dev. Biol. 86, 40-54.
99. Tinsley, R. C., and Kobel, H. R. (1996). "The biology of Xenopus." Oxford University Press, Oxford.
100. Tompkins, R. (1977). Grafting analysis of the periodic albino mutant of Xenopus laevis. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
101. Torpey, N., Wylie, C. C., and Heasman, J. (1992). Function of maternal cytokeratin in Xenopus development. Nature 357, 413-415.
102. Undamatla, J., and Szaro, B. G. (2001). Differential expression and localization of neuronal intermediate filament proteins within newly developing neurites in dissociated cultures of Xenopus laevis embryonic spinal cord. Cell Motil. Cytoskel. 49, 16-32.
103. Vize, P. D., Hemmati-Brivanlou, A., Harland, R. M., and Melton, D. A. (1991). Assays for gene function in developing Xenopus embryos. In "Methods in cell biology" (B. K. Kay and H. B. Peng, eds.), Vol. 36, pp. 368-388. Academic Press, San Diego.
104. Walker, K. L., Yoo, H.-K., Undamatla, J., and Szaro, B. G. (2001). Loss of neurofilaments alters axonal growth dynamics. J. Neurosci. 21, 9655-9666.
105. Warner, A. E., Guthrie, S. C., and Gilula, N. B. (1984). Antibodies to gap junctional protein selectively disrupt junctional communication in the early amphibian embryo. Nature 311, 127-131.
106. Weisblat, D. A., Sawyer, R. T., and Stent, G. S. (1978). Cell lineage analysis by intracellular injection of a tracer enzyme. Science 202, 1295-1298.
107. Wetzel, D. M., Lee, V. M. Y., and Erulkar, S. D. (1989). Long term cultures of neurons from adult frog brain express GABA and glutamate-activated channels. J. Neurobiol. 20, 255-270.
108. Wheeler, G. N., Hamilton, F. S., and Hoppler, S. (2000). Inducible gene expression in transgenic Xenopus embryos. Curr. Biol. 10, 849-852.
109. Williamson, T. L., Marszalek, J. R., Vechio, J. D., Bruijn, L. I., Lee, M. K., Xu, Z., Brown, R. H., Jr., and Cleveland, D. W. (1996). Neurofilaments, radial growth of axons, and mechanisms of motor neuron disease. Cold Spring Harbor Symp. Quant. Biol. 61, 709-723.
110. Wong, J., and Oblinger, M. M. (1990). Differential regulation of peripherin and neurofilament gene expression in regenerating rat DRG neurons. J. Neurosci. Res. 27, 332-341.
111. Wong, P. C., Marszalek, J., Crawford, T. O., Xu, Z. S., Hsieh, S. T., Griffin, J. W., and Cleveland, D. W. (1995). Increasing neurofilament subunit NF-M expression reduces axonal NF-H, inhibits radial growth, and results in neurofilamentous accumulation in motor neurons. J. Cell Biol. 130, 1413-1422.
112. Wu, M., and Gearhart, J. (1991). Raising Xenopus in the laboratory. Methods Cell Biol. 36, 3-18.
113. Yamasaki, H., Itakura, C., and Mizutani, M. (1991). Hereditary hypotrophic axonopathy with neurofilament deficiency in a mutant strain of the Japanese quail. Acta Neuropathol. 82, 427-434.
114. Zhao, J. X., Ohnishi, A., Itakura, C., Mizutani, M., Yamamoto, T., Hojo, T., and Murai, Y. (1995). Smaller axon and unaltered numbers of microtubules per axon in relation to number of myelin lamellae of myelinated fibers in the mutant quail deficient in neurofilaments. Acta Neuropathol. 89, 305-312.
115. Zhao, Y., and Szaro, B. G. (1994). The return of phosphorylated and nonphosphorylated epitopes of neurofilament proteins to the regenerating optic nerve of Xenopus laevis. J. Comp. Neurol. 343, 158-172.
116. Zhao, Y., and Szaro, B. G. (1995). The optic tract and tectal ablation influence the composition of neurofilaments in regenerating optic axons of Xenopus laevis. J. Neurosci. 15, 4629-4640.
117. Zhao, Y., and Szaro, B. G. (1997). Xefiltin, a Xenopus laevis neuronal intermediate filament protein, is expressed in actively growing optic axons during development and regeneration. J. Neurobiol. 33, 811-824.
118. Zhu, Q., Couillard-Despres, S., and Julien, J.-P. (1997). Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp. Neurol. 148, 299-316.
119. Zhu, Q., Lindenbaum, M., Levavasseur, F., Jacomy, H., and Julien, J.-P. (1998). Disruption of the NF-H gene increases axonal microtubule content and velocity of neurofilament transport: Relief of axonopathy resulting from the toxin β,β′-iminodipropionitrile. J. Cell Biol. 143, 183-193.