Cysteine-scanning mutagenesis of muscle carnitine palmitoyltransferase I reveals a single cysteine residue (Cys-305) is important for catalysis

Journal of Biological Chemistry

Volumn 280, Issue 6, 2005, Pages 4524-4531

  Liu, HongYan ^a,b   Zheng, Guolu ^a,c   Treber, Michelle ^a   Dai, Jia ^a   Woldegiorgis, Gebre ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CELLS; ENZYME KINETICS; FATTY ACIDS; MUSCLE; MUTAGENESIS; SENSITIVITY ANALYSIS; TRANSPORT PROPERTIES;

CARNITINE PALMITOYLTRANSFERASE (CPT); CYTOPLASM; LONG-CHAIN FATTY ACIDS; MUTANTS;

AMINO ACIDS;

ALANINE; CARNITINE PALMITOYLTRANSFERASE; CYSTEINE; MALONYL COENZYME A; PALMITOYL COENZYME A HYDROLASE; SERINE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME MECHANISM; FATTY ACID TRANSPORT; MITOCHONDRION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ALIGNMENT;

ALANINE; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BLOTTING, WESTERN; CARNITINE; CARNITINE O-PALMITOYLTRANSFERASE; CATALYSIS; CYSTEINE; DNA PRIMERS; HUMANS; KINETICS; MALONYL COENZYME A; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; MYOCARDIUM; PALMITIC ACID; PALMITOYLCARNITINE; PICHIA; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SERINE;

EID: 14244260908     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M400893200     Document Type: Article

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