Functional characterization of mammalian mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris

Journal of Nutrition

Volumn 130, Issue 2 SUPPL., 2000, Pages

  Woldegiorgis, Gebre ^a   Shi, Jianying ^a   Zhu, Hongfa ^a   Arvidson, Dennis N ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Binding residues; Carnitine palmitoyltransferases; Malonyl CoA inhibition; Pichia pastoris expression

Indexed keywords

ACYL COENZYME A; CARNITINE PALMITOYLTRANSFERASE; DETERGENT; FATTY ACID; MALONYL COENZYME A;

BINDING AFFINITY; CATALYSIS; CHEMICAL MUTAGENESIS; CONFERENCE PAPER; DELETION MUTANT; FATTY ACID OXIDATION; MITOCHONDRION; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION;

MAMMALIA; PICHIA PASTORIS;

EID: 0033985264     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/130.2.310s     Document Type: Conference Paper

References (44)

1. Adams, M. D., Kerlavage, A. R., Fuldner, R. A., Philips, C. A. & Venter, J. C. (1996) Complete sequence of a chromosome 22q subtelomeric BAC. Unpublished, Genbank Accession #U62317.
2. Adams, S. H., Esser, V., Brown, N. F., Ing, N. H., Johnson, L, Foster, D. W. & McGarry, J. D. (1998) Expression and possible role of muscle-type carnitine palmitoyltransferase I during sperm development in the rat. Biol. Reprod. 59: 1399-1405.
3. Bieber, L. L. (1988) Carnitine. Annu. Rev. Biochem. 57: 261-283.
4. Bonnefont, J. P., Taroni, F., Cavadini, P., Cepanec, C., Brivet, M., Saudubray, J. M., Leroux, J. P. & Demaugre, F. (1996) Molecular analysis of carnitine palmitoyltransferase II deficiency with hepatocardiomuscular expression. Am. J. Hum. Genet. 58: 971-978.
5. Brandt, J. M., Djouadi, F. & Kelly, D. P. (1998) Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator activated receptor α. J. Biol. Chem. 273: 23786-23792.
6. Bremer, J. (1981) The effect of fasting on the activity of liver carnitine palmitoyltransferase and its inhibition by malonyl CoA. Biochim. Biophys. Acta 665: 628-631.
7. Bremer, J., Woldegiorgis, G., Schalinske, K. & Shrago, E. (1985) Carnitine palmitoyltransferase: activation by palmitoyl CoA and inactivation by malonyl CoA. Biochim. Biophys. Acta 833: 9-16.
8. Britton, C. H., Schultz, R. A., Zhang, B., Esser, V., Foster, D. W. & McGarry, J. D. (1995) Human liver mitochondrial carnitine palmitoyltransferase I: characterization of its cDNA and chromosomal localization and partial analysis of the gene. Proc. Natl. Acad. Sci. U.S.A. 92: 1984-1988.
9. Brown, N. F., Anderson, R. C., Caplan, S. L, Foster, D. W. & McGarry, J. D. (1994a) Catalytically important domains of rat carnitine palmitoyltransferase II as determined by site-directed mutagenesis and chemical modification. J. Biol. Chem. 269: 19157-19162.
10. Brown, N. F., Esser, V., Foster, D. W. & McGarry, J. D. (1994b) Expression of a cDNA for rat liver carnitine palmitoyltransferase I in yeast establishes that the catalytic activity and malonyl-CoA sensitivity reside in a single polypeptide. J. Biol. Chem. 269: 26438-26442.
11. Brown, N. F., Sen, A., Soltis, D. A., Jones, B., Foster, D. W. & McGarry, J. D. (1993) Expression of precursor and mature carnitine palmitoyltransferase II in Escherichia coli and in vitro: differential behaviour of rat and human isoforms. Biochem. J. 294: 79-86.
12. Brown, N. F., Weis, B. C., Husti, J. E., Foster, D. W. & McGarry, J. D. (1995) Mitochondrial camitine palmitoyltransferase I isoform switching in the developing rat heart. J. Biol. Chem. 270: 8952-8957.
13. Chung, C. H., Woldegiorgis, G., Dai, G., Shrago, E. & Bieber, L. L. (1992) Conferral of malonyl coenzyme A sensitivity to purified rat heart mitochondrial camitine palmitoyltransferase. Biochemistry 31: 9777-9783.
14. Cohen, I., Kohl, C., McGarry, J. D., Girard, J. & Prip-Buus, C. (1998) The N-terminal domain of rat liver carnitine palmitoyltransferase I mediates import into the outer mitochondrial membrane and is essential for activity and malonyl CoA sensitivity. J. Biol. Chem. 273: 29896-29904.
15. 1 values for malonyl CoA. J. Biol. Chem. 262: 2050-2055.
16. Corr, P. B. & Yamada, K. A. (1995) Selected metabolic alterations in the ischemic heart and their contributions to arrhythmogenesis. Herz 20: 156-168.
17. de Vries, Y., Arvidson, D. N., Waterham, H., Cregg, J. M. & Woldegiorgis, G. (1997) Functional characterization of mitochondrial camitine palmitoyltransferases I and II expressed in the yeast P. pastoris. Biochemistry 36: 5285-5292.
18. Esser, V., Britton, C. H., Weis, B. C., Foster, D. W. & McGarry, J. D. (1993) Cloning, sequencing, and expression of a cDNA encoding rat liver carnitine palmitoyltransferase I. J. Biol. Chem. 268: 5817-5822.
19. Esser, V., Brown, N. F., Cowan, A. T., Foster, D. W. & McGarry, J. D. (1996) Expression of a cDNA isolated from rat brown adipose tissue and heart identifies the product as the muscle isoform of camitine palmitoyltransferase I (M-CPTI). J. Biol. Chem. 271: 6972-6977.
20. Finocchiaro, G., Taroni, F., Rocchi, M., Martin, A. L., Irma, C., Tarelli, G. T. & Didonato, S. (1991) cDNA cloning, sequence analysis, and chromosomal localization of the gene for human carnitine palmitoyltransferase. Proc. Natl. Acad. Sci. U.S.A. 88: 661-665.
21. Fraser, F., Corstorphine, C. G. & Zammit, V. A. (1997) Topology of carnitine palmitoyltransferase I in the mitochondrial outer membrane. Biochem. J. 323: 711-718.
22. Johnson, T. J., Mann, W. R., Dragland, C. J., Anderson, R. C., Nemecek, G. M. & Bell, P. A. (1995) Over-expression and characterization of active recombinant rat liver carnitine palmitoyltransferase II using baculovirus. Biochem. J. 309: 689-693.
23. Lund, H. & Woldegiorgis, G. (1987) Carnitine palmitoyltransferase: separation of enzyme activity and malonyl CoA binding in rat liver mitochondria. Biochim. Biophys. Acta 878: 243-249.
24. Mascaro, C., Acosta, E., Ortiz, J. A., Marrero, P. F., Hegardt, F. G. & Haro, D. (1998) Control of human muscle type carnitine palmitoyltransferase I gene transcription by peroxisome proliferator activated receptor. J. Biol. Chem. 273: 8560-8563.
25. McGarry, J. D. & Brown, N. F. (1997) The mitochondrial carnitine palmitoyltransferase system: from concept to molecular analysis. Eur. J. Biochem. 244: 1-14.
26. McGarry, J. D., Woeltje, K. F., Kuwajima, M. & Foster, D. W. (1989) Regulation of ketogenesis and the renaissance of camitine palmitoyltransferase. Diabetes Metab. Rev. 5: 271-284.
27. Power, G. W. & Newsholme, E. A. (1997) Dietary fatty acids influence the activity and metabolic control of mitochondrial camitine palmitoyltransferase I in rat heart and skeletal muscle. J. Nutr. 127: 2142-2150.
28. Prentki, M. & Corkey, B. E. (1996) Are the β-cell signaling molecules malonyl-CoA and cytosolic long-chain acyl-CoA implicated in multiple tissue defects of obesity and NIDDM? Diabetes 45: 273-283.
29. Prip-Buus, C., Cohen, I., Kohl, C., Esser, V., McGarry, J. D. & Girard, J. (1998) Topological and functional analysis of the rat liver carnitine palmitoyltransferase I expressed in Saccharomyces cerevisiae. FEBS Lett. 429: 173-178.
30. Shi, J., Zhu, H., Arvidson, D. N., Cregg, J. M. & Woldegiorgis, G. (1998) Deletion of the conserved first 18 N-terminal amino acid residues in rat liver carnitine palmitoyltransferase I abolishes malonyl CoA sensitivity and binding. Biochemistry 37: 11033-11038.
31. Shi, J., Zhu, H., Arvidson, D. N. & Woldegiorgis, G. (1999) A single amino acid change (substitution of glutamate 3 with alanine) in the N-terminal region of rat liver carnitine palmitoyltransferase I abolishes malonyl CoA inhibition and high affinity binding. J. Biol. Chem. 274: 9421-9426.
32. Swanson, S. T., Foster, D. W., McGarry, J. D. & Brown, N. F. (1998) Roles of the N- and C-terminal domains of carnitine palmitoyltransferase I isoforms in malonyl CoA sensitivity of the enzymes: insights from expression of chimaeric proteins and mutation of conserved histidine residues. Biochem. J. 335: 513-519.
33. Wang, D., Harrison, W., Buja, L. M., Elder, F.F.B. & McMillin, J. B. (1998) Genommic DNA sequence, promoter expression, and chromosomal mapping of rat muscle carnitine palmitoyltransferase I. Genomics 48: 314-323.
34. Weis, B. C., Cowan, A. T., Brown, N., Foster, D. W. & McGarry, J. D. (1994) Use of a selective inhibitor of liver carnitine palmitoyltransferase I (CRT I) allows quantification of its contribution to total CPT I activity in rat heart: Evidence that the dominant cardiac CRT I isoform is identical to the skeletal muscle enzyme. J. Biol. Chem. 269: 26443-26448.
35. Whitmer, J. T., Idell-Wenger, J. A., Rovetto, M. J. & Neely, J. R. (1978) Control of fatty acid metabolism in ischemic and hypoxic hearts. J. Biol. Chem. 253: 4305-4309.
36. Woeltje, K. F., Esser, V., Weis, B. C., Sen, A., Cox, W. F., McPhaul, M. J., Slaughter, C. A., Foster, D. W. & McGarry, J. D. (1990) Cloning, sequencing, and expression of a cDNA encoding rat liver mitochondrial carnitine palmitoyltransferase. J. Biol. Chem. 265: 10720-10725.
37. Woldegiorgis, G., Brenner, J. & Shrago, E. (1985) Substrate inhibition of carnitine palmitoyltransferase by palmitoyl CoA and activation by phospholipids and proteins. Biochim. Biophys. Acta 837: 135-140.
38. Woldegiorgis, G., Fibich, B., Contreras, L. & Shrago, E. (1992) Restoration of malonyl CoA sensitivity of soluble rat liver mitochondria carnitine palmitoyltransferase by reconstitution with a partially purified malonyl CoA binding protein. Arch. Biochem. Biophys. 295: 348-351.
39. Yamazaki, N., Shinhara, Y., Shima, A. & Terada, H. (1995) High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone. FEBS Lett. 363: 41-45.
40. Yamazaki, N., Shinhara, Y., Shima, A., Yamanaka, Y. & Terada, H. (1996) Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I. Biochim. Biophys. Acta 1307: 157-161.
41. Yu, G.-S., Lu, Y.-C. & Gulick, T. (1998a) Co-regulation of tissue-specific alternative human carnitine palmitoyltransferase Iβ gene promoters by fatty acid enzyme substrate. J. Biol. Chem. 273: 32901-32909.
42. Yu, G.-S., Lu, Y.-C. & Gulick, T. (1998b) Expression of novel isoforms of carnitine palmitoyltransferase I (CPT-I) generated by alternative splicing of the CPTI-Iβ gene. Biochem. J. 334: 225-231.
43. Zhu, H., Shi, J., Cregg, J. M. & Woldegiorgis, G. (1997a) Reconstitution of highly expressed human heart muscle carnitine palmitlytransferase I. Biochem. Biophys. Res. Commun. 239: 498-502.
44. Zhu, H., Shi, J., de Vries, Y., Arvidson, D. N., Cregg, J. M. & Woldegiorgis, G. (1997b) Functional studies of yeast expressed human heart muscle carnitine palmitoyltransferase I. Arch. Biochem. Biophys. 347: 53-61.