Human receptor Smoothened, a mediator of Hedgehog signalling, expressed in its native conformation in yeast

FEBS Letters

Volumn 579, Issue 6, 2005, Pages 1529-1533

  De Rivoyre, Matthieu ^a   Bonino, Frédéric ^a   Ruel, Laurent ^b   Bidet, Michel ^a   Thérond, Pascal ^b   Mus Veteau, Isabelle ^a  

^a CNRS   (France)

^b CENTRE DE BIOCHIMIE   (France)

Author keywords

Hedgehog signalling; Heterologous expression; Membrane protein; Purification; Smoothened; Yeast

Indexed keywords

SONIC HEDGEHOG PROTEIN;

ARTICLE; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; YEAST;

ERINACEIDAE; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 14244253570     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2005.01.054     Document Type: Article

References (31)

1. P.W. Ingham, and A.P. McMahon Hedgehog signaling in animal development: paradigms and principles Genes Dev. 15 2001 3059 3087
2. J.E. Hooper, and M.P. Scott The Drosophila patched gene encodes a putative membrane protein required for segmental patterning Cell 59 1989 751 765
3. D.M. Stone The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog Nature 384 1996 129 134
4. N. Fuse, T. Maiti, B. Wang, J.A. Porter, T.M. Hall, D.J. Leahy, and P.A. Beachy Sonic hedgehog protein signals not as a hydrolytic enzyme but as an apparent ligand for patched Proc. Natl. Acad. Sci. USA 96 1999 10992 10999
5. D. Kalderon Transducing the hedgehog signal Cell 103 2000 371 374
6. J. Taipale, and P.A. Beachy The Hedgehog and Wnt signalling pathways in cancer Nature 411 2001 349 354
7. J.K. Chen, J. Taipale, M.K. Cooper, and P.A. Beachy Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened Genes Dev. 16 2002 2743 2748
8. J.K. Chen, J. Taipale, K.E. Young, T. Maiti, and P.A. Beachy Small molecule modulation of Smoothened activity Proc. Natl. Acad. Sci. USA 99 2002 14071 14076
9. L. Ruel, R. Rodriguez, A. Gallet, L. Lavenant-Staccini, and P.P. Therond Stability and association of Smoothened, Costal2 and Fused with Cubitus interruptus are regulated by Hedgehog Nat. Cell Biol. 5 2003 907 913
10. L. Lum Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2 Mol. Cell 12 2003 1261 1274
11. K. Nybakken, and N. Perrimon Hedgehog signal transduction: recent findings Curr. Opin. Genet. Dev. 12 2002 503 511
12. J.P. Incardona, J. Gruenberg, and H. Roelink Sonic hedgehog induces the segregation of patched and smoothened in endosomes Curr. Biol. 12 2002 983 995
13. J. Taipale, M.K. Cooper, T. Maiti, and P.A. Beachy Patched acts catalytically to suppress the activity of Smoothened Nature 418 2002 892 897
14. T. Reya, S.J. Morrison, M.F. Clarke, and I.L. Weissman Stem cells, cancer, and cancer stem cells Nature 414 2001 105 111
15. A. Ruiz i Altaba, P. Sanchez, and N. Dahmane Gli and hedgehog in cancer: tumours, embryos and stem cells Nat. Rev. Cancer 2 2002 361 372
16. K. Lai, B.K. Kaspar, F.H. Gage, and D.V. Schaffer Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo Nat. Neurosci. 6 2003 21 27
17. C.G. Tate Overexpression of mammalian integral membrane proteins for structural studies FEBS Lett. 504 2001 94 98
18. I. Mus-Veteau Heterologous expression and purification systems for structural proteomics of mammalian membrane proteins Comp. Funct. Genom. 3 2002 511 517
19. R.A. Figler, H. Omote, R.K. Nakamoto, and M.K. Al-Shawi Use of chemical chaperones in the yeast Saccharomyces cerevisiae to enhance heterologous membrane protein expression: high-yield expression and purification of human P-glycoprotein Arch. Biochem. Biophys. 376 2000 34 46
20. G. Lennon, C. Auffray, M. Polymeropoulos, and M.B. Soares The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression Genomics 33 1996 151 152
21. J.M. Cregg, K.J. Barringer, A.Y. Hessler, and K.R. Madden Pichia pastoris as a host system for transformations Mol. Cell Biol. 5 1985 3376 3385
22. 2+-transporting ATPases Am. J. Physiol. Cell Physiol. 287 2004 C580 C589
23. R. Yelin, and S. Schuldiner Vesicular monoamine transporters heterologously expressed in the yeast Saccharomyces cerevisiae display high-affinity tetrabenazine binding Biochim. Biophys. Acta 1510 2001 426 441
24. G. Lenoir, T. Menguy, F. Corre, C. Montigny, P.A. Pedersen, D. Thines, M. le Maire, and P. Falson Overproduction in yeast and rapid and efficient purification of the rabbit SERCA1a Ca(2+)-ATPase Biochim. Biophys. Acta 1560 2002 67 83
25. J.L. Cereghino, and J.M. Cregg Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol. Rev. 24 2000 45 66
26. I.J. van der Klei, W. Harder, and M. Veenhuis Biosynthesis and assembly of alcohol oxidase, a peroxisomal matrix protein in methylotrophic yeasts: a review Yeast 7 1991 195 209
27. J. Cai, and P. Gros Overexpression, purification, and functional characterization of ATP-binding cassette transporters in the yeast, Pichia pastoris Biochim. Biophys. Acta 1610 2003 63 76
28. L.A. de Jong, S. Grunewald, J.P. Franke, D.R. Uges, and R. Bischoff Purification and characterization of the recombinant human dopamine D2S receptor from Pichia pastoris Protein Expr. Purif. 33 2004 176 184
29. L. Beaudet, I.L. Urbatsch, and P. Gros High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity Methods Enzymol. 292 1998 397 413
30. R.T. Niebauer, A. Wedekind, and A.S. Robinson Decreases in yeast expression yields of the human adenosine A2a receptor are a result of translational or post-translational events Protein Expr. Purif. 37 2004 134 143
31. H.M. Weiss, and R. Grisshammer Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli Eur. J. Biochem. 269 2002 82 92