Direct in vivo VH to JH rearrangement violating the 12/23 rule

Journal of Experimental Medicine

Volumn 201, Issue 3, 2005, Pages 341-348

  Koralov, Sergei B ^a   Novobrantseva, Tatiana I ^a,c   Hochedlinger, Konrad ^b   Jaenisch, Rudolf ^b   Rajewsky, Klaus ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^c BIOGEN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN; ANTIBODY;

ALLELE; ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; GENE LOCUS; GENE REARRANGEMENT; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN VARIABLE REGION; IN VIVO STUDY; MOUSE; MOUSE STRAIN; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; ANIMAL; BAGG ALBINO MOUSE; C57BL MOUSE; GENETIC RECOMBINATION; GENETICS; METABOLISM; PHYSIOLOGY;

ANIMALS; ANTIBODIES; B-LYMPHOCYTE SUBSETS; B-LYMPHOCYTES; GENE REARRANGEMENT, B-LYMPHOCYTE, HEAVY CHAIN; GENES, IMMUNOGLOBULIN; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; RECOMBINATION, GENETIC;

EID: 13644267730     PISSN: 00221007     EISSN: None     Source Type: Journal    
DOI: 10.1084/jem.20041577     Document Type: Article

References (27)

1. Roth, D.B. 2003. Restraining the V(D)J recombinase. Nat. Rev. Immunol. 3:656-666.
2. Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature. 302:575-581.
3. Jones, J.M., and M. Gellert. 2002. Ordered assembly of the V(D)J synaptic complex ensures accurate recombination. EMBO J. 21:4162-4171.
4. Sawchuk, D.J., F. Weis-Garcia, S. Malik, E. Besmer, M. Bustin, M.C. Nussenzweig, and P. Cortes. 1997. V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins. J. Exp. Med. 185:2025-2032.
5. Schlissel, M., A. Constantinescu, T. Morrow, M. Baxter, and A. Peng. 1993. Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated, RAG-dependent, and cell cycle regulated. Genes Dev. 7:2520-2532.
6. Hochedlinger, K., and R. Jaenisch. 2002. Monoclonal mice generated by nuclear transfer from mature B and T donor cells. Nature. 415:1035-1038.
7. Kitamura, D., A. Kudo, S. Schaal, W. Muller, F. Melchers, and K. Rajewsky. 1992. A critical role of lambda 5 protein in B cell development. Cell. 69:823-831.
8. Hao, Z., and K. Rajewsky. 2001. Homeostasis of peripheral B cells in the absence of B cell influx from the bone marrow. J. Exp. Med. 194:1151-1164.
9. Relink, A.G., C. Schaniel, J. Andersson, and F. Melchers. 2001. Selection events operating at various stages in B cell development. Curr. Opin. Immunol. 13:202-207.
10. Hayakawa, K., R.R. Hardy, A.M. Stall, and L.A. Herzenberg. 1986. Immunoglobulin-bearing B cells reconstitute and maintain the murine Ly-1 B cell lineage. Eur. J. Immunol. 16:1313-1316.
11. Gu, H., Y.R. Zou, and K. Rajewsky. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164.
12. Ehlich, A., V. Martin, W. Muller, and K. Rajewsky. 1994. Analysis of the B-cell progenitor compartment at the level of single cells. Curr. Biol. 4:573-583.
13. Ye, J. 2004. The immunoglobulin IGHD gene locus in C57BL/6 mice. Immunogenetics. 56:399-404.
14. Kepler, T.B., M. Borrero, B. Rugerio, S.K. McCray, and S.H. Clarke. 1996. Interdependence of N nucleotide addition and recombination site choice in V(D)J rearrangement. J. Immunol. 157:4451-4457.
15. Kantor, A.B., C.E. Merrill, L.A. Herzenberg, and J.L. Hillson. 1997. An unbiased analysis of V(H)-D-J(H) sequences from B-1a, B-1b, and conventional B cells. J. Immunol. 158:1175-1186.
16. Martin, F., and J.F. Kearney. 2000. B-cell subsets and the mature pre-immune repertoire. Marginal zone and B1 B cells as part of a "natural immune memory". Immunol. Rev. 175:70-79.
17. Siebert, P.D., A. Chenchik, D.E. Kellogg, K.A. Lukyanov, and S.A. Lukyanov. 1995. An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res. 23:1087-1088.
18. Forster, I., and K. Rajewsky. 1987. Expansion and functional activity of Ly-1+ B cells upon transfer of peritoneal cells into allotype-congenic, newborn mice. Eur. J. Immunol. 17:521-528.
19. Hooper, M., K. Hardy, A. Handyside, S. Hunter, and M. Monk. 1987. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature. 326:292-295.
20. Loffert, D., A. Ehlich, W. Muller, and K. Rajewsky. 1996. Surrogate light chain expression is required to establish immunoglobulin heavy chain allelic exclusion during early B cell development. Immunity. 4:133-144.
21. Sonoda, E., Y. Pewzner-Jung, S. Schwers, S. Taki, S. Jung. D. Eilat, and K. Rajewsky. 1997. B cell development under the condition of allelic inclusion. Immunity. 6:225-233.
22. Kabat, E.A., and T.T. Wu. 1991. Identical V region amino acid sequences and segments of sequences in antibodies of different specificities. Relative contributions of VH and VL genes, minigenes, and complementarity-determining regions to binding of antibody-combining sites. J. Immunol. 147:1709-1719.
23. Lefranc, M.P. 2001. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 29:207-209.
24. Lefranc, M.P., V. Giudicelli, C. Ginestoux, J. Bodmer, W. Muller, R. Bontrop, M. Lemaitre, A. Malik, V. Barbie, and D. Chaume. 1999. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 27:209-212.
25. Lefranc, M.P. 2003. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 31:307-310.
26. Ruiz, M., V. Giudicelli, C. Ginestoux, P. Stoehr, J. Robinson, J. Bodmer, S.G. Marsh, R. Bontrop, M. Lemaitre, G. Lefranc, et al. 2000. IMGT, the international ImMunoGeneTics database. Nucleic Acids Res. 28:219-221.
27. Ford, J.E., M.G. McHeyzer-Williams, and M.R. Lieber. 1994. Chimeric molecules created by gene amplification interfere with the analysis of somatic hypermutation of murine immunoglobulin genes. Gene. 142:279-283.