Single kinesin molecules studied with a molecular force clamp

Nature

Volumn 400, Issue 6740, 1999, Pages 184-189

  Visscher, Koen ^a   Schnltzer, Mark J ^a   Block, Steven M ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; KINESIN;

ARTICLE; FORCE; HYDROLYSIS; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; PROTEIN ANALYSIS;

ADENOSINE TRIPHOSPHATE; BIOMECHANICS; HYDROLYSIS; KINESIN; LASERS; MICROSPHERES; MOLECULAR MOTOR PROTEINS;

EID: 0033536183     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/22146     Document Type: Article

References (32)

1. Howard, J. Molecular motors: structural adaptations to cellular functions. Nature 389, 561-567 (1997).
2. Howard, J. The movement of kinesin along microtubules. Annu. Rev. Physiol. 58, 703-729 (1996).
3. Svoboda, K., Schmidt, C. F., Schnapp, B. J. & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727 (1993).
4. Howard, J., Hudspeth, A. J. & Vale, R. D. Movement of microtubules by single kinesin molecules. Nature 342, 154-158 (1989).
5. Visscher, K. & Block, S. M. Versatile optical traps with feedback control. Methods Enzymol. 298, 460-489 (1998).
6. Funatsu, T., Harada, Y., Tokunaga, M., Saito, K. & Yanagida, T. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature 374, 555-559 (1995).
7. Ishijima, A. et al. Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin. Cell 92, 161-171 (1998).
8. Schnitzer, M. J. & Block, S. M. Kinesin hydrolyses one ATP per 8-nm step. Nature 388, 386-390 (1997).
9. Hua, W., Young, E. C., Fleming, M. L. & Gelles, J. Coupling of kinesin steps to ATP hydrolysis. Nature 388, 390-393 (1997).
10. Svoboda, K. & Block, S. M. Force and velocity measured for single kinesin molecules. Cell 77, 773-784 (1994).
11. Kojima, H., Muto, E., Higuchi, H. & Yanagida, T. Mechanics of single kinesin molecules measured by optical trapping nanometry. Biophys. J. 73, 2012-2022 (1997).
12. Coppin, C. M., Pierce, D. W., Hsu, L. & Vale, R. D. The load dependence of kinesin's mechanical cycle. Proc. Natl Acad. Sci. USA 94, 8539-8544 (1997).
13. Meyhöfer, E. & Howard, J. The force generated by a single kinesin molecule against an elastic load. Proc. Natl Acad. Sci. USA 92, 574-578 (1995).
14. Visscher, K., Gross, S. P. & Block, S. M. Construction of multiple-beam optical traps with nanometer-resolution position sensing. IEEE J. Sel. Topics Quantum. Electron. 2, 1066-1076 (1996).
15. Svoboda, K. & Block, S. M. Biological applications of optical forces. Annu. Rev. Biophys. Biomol. Struct. 23, 247-285 (1994).
16. Finer, J. T., Simmons, R. M. & Spudich, J. A. Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature 368, 113-119 (1994).
17. Wang, M. D., Yin, H., Landick, R., Gelles, J. & Block, S. M. Stretching DNA with optical tweezers. Biophys. J. 72, 1335-1346 (1996).
18. Wang, M. D. et al. Force and velocity measured for single molecules of RNA polymerase. Science 282, 902-907 (1998).
19. Howard, J. The mechanics of force generation by kinesin. Biophys. J. 68, 245s-255s (1995).
20. Hackney, D. Kinesin ATPase: Rate-limiting ADP release. Proc. Natl Acad. Sci. USA 85, 6314-4318 (1988).
21. Gilbert, S. P., Webb, M. R., Brune, M. & Johnson, K. A. Pathway of processive ATP hydrolysis by kinesin. Nature 373, 671-676 (1995).
22. Ma, Y. Z. & Taylor, E. W. Mechanism of microtubule kinesin ATPase. Biochemistry 34, 13242-13251 (1995).
23. Svoboda, K., Mitra, P. P. & Block, S. M. Fluctuation analysis of motor protein movement and single enzyme kinetics. Proc. Natl Acad. Sci. USA 91, 11782-11786 (1994).
24. Schnitzer, M. J. & Block, S. M. Statistical kinetics of processive enzymes. Cold Spring Harbor Symp. Quant. Biol. 60, 793-802 (1995).
25. Samuel, A. D. T. & Berg, H. C. Torque-generating units of the bacterial flagellar motor step independently. Biophys. J. 71, 918-923 (1996).
26. Leibler, S. & Huse, D. A. Porters versus rowers: a unified stochastic model of motor proteins. J. Cell Biol. 121, 1357-1368 (1993).
27. Peskin, C. & Oster, G. Coordinated hydrolysis explains the mechanical behavior of kinesin. Biophys. J. 68, 202s-211s (1995).
28. Duke, T. & Leibler, S. Motor protein mechanics: a stochastic model with minimal mechanochemical coupling. Biophys. J. 71, 1235-1247 (1996).
29. Derényi, I. & Vicsek, T. The kinesin walk: a dynamic model with elastically coupled heads. Proc. Natl Acad. Sci. USA 93, 6775-6779 (1996).
30. Astumian, R. D. Thermodynamics and kinetics of a brownian motor. Science 276, 917-922 (1997).
31. Higuchi, H. & Yanagida, T. Force generation and detachment of single kinesin molecules activated by laser photolysis of caged ATP and ADP. Cell Struct. Funct. 23, suppl., 198 (1998).
32. Coppin, C. M., Finer, J. T., Spudich, J. A. & Vale, R. D. Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy. Proc. Natl Acad. Sci. USA 93, 1913-1917 (1996).