Construction and structural modeling of a single-chain Fv-asparaginese fusion protein resistant to proteolysis

Biotechnology and Bioengineering

Volumn 70, Issue 4, 2000, Pages 456-463

  Guo, Li ^a   Wang, Jinhua ^b   Qian, Shijun ^a   Yan, Xiyun ^a   Chen, Runsheng ^a   Meng, Guangzhen ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b INSTITUTE OF BIOPHYSICS   (China)

Author keywords

Bacterial expression; Fusion protein; L asparaginase; Recombinant DNA technology; Resistance to proteolysis; Single chain Fv (scFv); Three dimensional structure modeling

Indexed keywords

ANTIBODIES; ENZYME KINETICS; ESCHERICHIA COLI; MOLECULAR STRUCTURE; PROTEINS;

FUSION PROTEINS; PROTEOLYSIS; THERAPEUTIC PROTEINS;

GENETIC ENGINEERING;

ANTIBODY; ASPARAGINASE; ASPARAGINE; HYBRID PROTEIN; LINK PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; CELL INCLUSION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE LIBRARY; HALF LIFE TIME; MOLECULAR MODEL; NONHUMAN; PHAGE DISPLAY; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RECOMBINANT PLASMID; STEREOSPECIFICITY;

ASPARAGINASE; BASE SEQUENCE; ELECTROSTATICS; GENE LIBRARY; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034694204     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001120)70:4<456::AID-BIT11>3.0.CO;2-E     Document Type: Article

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