Long-term maintenance of human keratinocytes in vitro [1]

Journal of Investigative Dermatology

Volumn 124, Issue 2, 2005, Pages 475-478

  Hasskarl, Jens ^a,b   Velupillai, Palanivel ^a   Piboonniyom, Siribang On ^a,c   Grace, Miranda ^a   Münger, Karl ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF FREIBURG   (Germany)

^c MAHIDOL UNIVERSITY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA6BETA4 INTEGRIN; CD34 ANTIGEN; CD71 ANTIGEN; MITOMYCIN C; THYMIDINE;

CELL CULTURE; CELL POPULATION; CELL PROLIFERATION; CELL SELECTION; CLONOGENESIS; FLUORESCENCE ACTIVATED CELL SORTING; HAIR FOLLICLE; HYDROPHOBICITY; IN VITRO STUDY; KERATINOCYTE; LETTER; NONHUMAN; PRIORITY JOURNAL; SKIN FIBROBLAST;

EID: 12944286449     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-202X.2004.23574.x     Document Type: Letter

References (23)

1. Barrandon Y, Green H: Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci USA 34:2302-2306, 1987
2. Bickenbach JR: Identification and behavior of label-retaining cells in oral mucosa and skin. J Dent Res 60 (Spec No C):1611-1620, 1981
3. Cotsarelis G, Sun TT, Lavker RM: Label-retaining cells reside in the bulge area of pilosebaceous unit: Implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell 61:1329-1337, 1990
4. Dimri GP: A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA 92:9363-9367, 1995
5. Hasskarl J, Duensing S, Manuel E, Munger K: The helix-loop-helix protein ID1 localizes to centrosomes and rapidly induces abnormal centrosome numbers. Oncogene 23:1930-1938, 2004
6. Jones PH, Watt FM: Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 73:713-724, 1993
7. Kaur P, Li A: Adhesive properties of human basal epidermal cells: An analysis of keratinocyte stem cells, transit amplifying cells, and postmitotic differentiating cells. J Invest Dermatol 114:413-420, 2000
8. Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ: Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396:84-88, 1998
9. Lundberg AS, Hahn WC, Gupta P, Weinberg RA: Genes involved in senescence and immortalization. Curr Opin Cell Biol 12:705-709, 2000
10. Masutomi K, Yu EY, Khurts S, et al: Telomerase maintains telomere structure in normal human cells. Cell 114:241-253, 2003
11. Mercurio AM, Rabinovitz I, Shaw LM: The alpha 6 beta 4 integrin and epithelial cell migration. Curr Opin Cell Biol 13:541-545, 2001
12. Meyers C, Frattini MG, Laimins LA: Tissue culture techniques for the study of human papillomaviruses in stratified epithelia. In: Celis JE (ed) Cell Biology: A Laboratory Handbook, Vol. 1. San Diego, CA, USA: Academic Press, 1994; p 491
13. Morris RJ, Liu Y, Maries L, et al: Capturing and profiling adult hair follicle stem cells. Nat Biotechnol 22:411-417, 2004
14. Papini S, Cecchetti D, Campani D, et al: Isolation and clonal analysis of human epidermal keratinocyte stem cells in long-term culture. Stem Cells, 2003
15. Potten CS, Morris RJ: Epithelial stem cells in vivo. J Cell Sci Suppl 10:45-62, 1988
16. Ramirez RD, Morales CP, Herbert BS, Rohde JM, Passons C, Shay JW, Wright WE: Putative telomere-independent mechanisms of replicative aging reflect inadequate growth conditions. Genes Dev 15:398-403, 2001
17. Rheinwald JG: Serial cultivation of normal human epidermal keratinocytes. Methods Cell Biol 21A:229-254, 1980
18. Rheinwald JG, Beckett MA: Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22:629-632, 1980
19. Stewart SA, Ben-Porath I, Carey VJ, O'Connor BF, Hahn WC, Weinberg RA: Erosion of the telomeric single-strand overhang at replicative senescence. Nat Genet 33:492-496, 2003
20. Tani H, Morris RJ, Kaur P: Enrichment for murine keratinocyte stem cells based on cell surface phenotype. Proc Natl Acad Sci USA 97:10960-10965, 2000
21. Taylor G, Lehrer MS, Jensen PJ, Sun TT, Lavker RM: Involvement of follicular stem cells in forming not only the follicle but also the epidermis. Cell 102: 451-461, 2000
22. Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW: Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. J Invest Dermatol 120: 501-511, 2003
23. Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E: Defining the epithelial stem cell niche in skin. Science 303:359-363, 2004