Isolation and clonal analysis of human epidermal keratinocyte stem cells in long-term culture

Stem Cells

Volumn 21, Issue 4, 2003, Pages 481-494

  Papini, Sandra ^a   Cecchetti, Denise ^b   Campani, Daniela ^b   Fitzgerald, Wendy ^c   Grivel, Jean Charles ^c   Chen, Silvia ^c   Margolis, Leonid ^c   Revoltella, Roberto P ^a  

^a CNR   (Italy)

^b UNIVERSITY OF PISA   (Italy)

^c NASA HEADQUARTERS   (United States)

Author keywords

Cell cloning; Differentiation; Keratinocyte; Stem cells; Three dimensional cultures

Indexed keywords

COLLAGEN; GELATIN; MUCIN;

CELL CLONE; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL PROLIFERATION; CONFERENCE PAPER; CULTURE MEDIUM; EPIDERMIS CELL; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; IRRADIATION; KERATINOCYTE; PREPUCE; SENESCENCE; STEM CELL;

EID: 0038449819     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.21-4-481     Document Type: Conference Paper

References (53)

1. Green H. The keratinocyte as differentiated cell type. Harvey Lect 1980;74:101-139.
2. Watt FM. Terminal differentiation of epidermal keratinocytes. Curr Opin Cell Biol 1989;6:1107-1115.
3. De Luca M, Tamura RN, Kajiji S et al. Polarized integrin mediates human keratinocyte adhesion to basal lamina. Proc Natl Acad Sci USA 1990;87:6888-6892.
4. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell 1992;69:11-25.
5. Fuchs E, Byrne C. The epidermis: rising to the surface. Curr Opin Genet Dev 1994;4:725-736.
6. Jones JC, Asmuth J, Baker SE et al. Hemidesmosomes: extracellular matrix/intermediate filament connectors. Exp Cell Res 1994;213:1-11.
7. Roop D. Defects in the barrier. Science 1995;267:474-475.
8. Christiano AM, Uitto J. Molecular complexity of the cutaneous basement membrane zone. Revelations from the paradigms of epidermolysis bullosa. Exp Dermatol 1996;5:1-11.
9. Borradori L, Sonnenberg A. Hemidesmosomes: roles in adhesion, signaling and human diseases. Curr Opin Cell Biol 1996;8:647-656.
10. Dowling J, Yu QC, Fuchs E. Beta4 integrin is required for hemidesmosome formation, cell adhesion and cell survival. J Cell Biol 1996;134:559-572.
11. Lavker RM, Sun TT. Epidermal stem cells. J Invest Dermatol 1983;81(suppl 1):121-127.
12. Miller SJ, Lavker RM, Sun TT. Keratinocyte stem cells of cornea skin and hair follicles. In: Potten CS, ed. Stem Cells. San Diego, CA: Academic Press Inc., 1997:331-362.
13. Barrandon Y, Green H. Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci USA 1987;84:2302-2306.
14. Barrandon Y, Green H. Three clonal types of keratinocytes with different capacities of differentiation. Proc Natl Acad Sci USA 1993;84:209-215.
15. Barrandon Y. The epidermal stem cell: an overview. Dev Biol 1993;4:209-214.
16. Jones PH, Watt FM. Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 1993;73:713-724.
17. Rochat A, Kobayashi K, Barrandon Y. Locafion of stem cells of human hair follicles by clonal analysis. Cell 1994;76:1063-1073.
18. Mathor MB, Ferrari G, Dellambra E et al. Clonal analysis of stably transduced human epidermal stem cells in culture. Proc Natl Acad Sci USA 1996;93:10371-10376.
19. Pellegrini G, Golisano O, Paterna P et al. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol 1999;145:769-782.
20. Fusenig NE. Epithelial-mesenchymal interactions regulate keratinocyte growth and differentiation in vitro. In: Leigh IM, Lane EB, Watt FM, eds. The Keratinocytes Handbook. Cambridge: Cambridge University Press, 1994:71-94.
21. Eady RA, Dunnill MG. Epidermolysis bullosa: hereditary skin fragility diseases as paradigms in cell biology. Arch Dermatol Res 1994;287:2-9.
22. Boyce ST. Epidermis as a secretory tissue. J Invest Dermatol 1994;102:8-10.
23. Fenjves ES, Gordon DA, Pershing LK et al. Systemic distribution of apolipoprotein E secreted by grafts of epidermal keratinocytes: implications for epidermal function and gene therapy. Proc Natl Acad Sci USA 1989;86:8803-8807.
24. Alexander MY, Bidichandani SI, Cousins FM et al. Circulating human factor IX produced in keratin-promoter transgenic mice: a feasibility study for gene therapy of haemophilia B. Hum Mol Genet 1995;4:993-999.
25. Cuono C, Langdon R, McGuire J. Use of cultured epidermal autografts and dermal allografts as skin replacement after bum injury. Lancet 1986;1:1123-1124.
26. De Luca M, D'Anna F, Bondanza S et al. Human epithelial cells induce human melanocyte growth in vitro but only skin keratinocytes regulate its proper differentiation in the absence of dermis. J Cell Biol 1988;107:1919-1926.
27. Compton CC, Gill JM, Bradford DA et al. Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting. A light electron microscopic and immunohistochemical study. Lab Invest 1989;60:600-612.
28. De Luca M, Albanese E, Bondanza S et al. Multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium fresh or preserved in a frozen state. Burns 1989;15:303-309.
29. Gallico GG 3rd, O'Connor NE, Compton CC et al. Permanent coverage of large bum wounds with autologous cultured human epithelium. N Engl J Med 1984;311:448-451.
30. Matoušková E, Veselý P, Königová R. Modified method of in vitro cultivation of human keratinocytes suitable for grafting. Folia Biol (Praha) 1989;35:267-271.
31. Matoušková E, Vogtová D, Königovä R. A recombined skin composed of human keratinocytes cultured on cell-free pig dermis. Burns 1993;19:118-123.
32. Teumer J, Lindhal A, Green H. Human growth hormone in the blood of athymic mice grafted with cultures of hormone-secreting human keratinocytes. FASEB J 1990;4:3245-3250.
33. Fenjves ES. Approaches to gene transfer in keratinocytes. J Invest Dermatol 1994;103(suppl 5):70-75.
34. Krueger GG, Morgan JR, Jorgensen CM et al. Genetically modified skin to treat disease: potential and limitations. J Invest Dermatol 1994;103(suppl 5):76S-84S.
35. Greenhalgh DA, Rothnagel JA, Roop DR. Epidermis: an attractive target tissue for gene therapy. J Invest Dermatol 1994;103(suppl 5):63S-69S.
36. Vogt PM, Thompson S, Andree C et al. Genetically modified keratinocytes transplanted to wounds reconstitute the epidermis. Proc Natl Acad Sci USA 1994;91:9307-9311.
37. Dellambra E, Vailly J, Pellegrini G et al. Corrective transduction of human epidermal stem cells in laminin-5-dependent junctional epidermolysis bullosa. Hum Gene Ther 1998;9:1359-1370.
38. Pellegrini G, Bondanza S, Guerra L et al. Cultivation of human keratinocyte stem cells: current and future clinical applications. Cell Eng 1998;36:1-13.
39. Parenteau NL, Bilbo P, Nolte CJM et al. The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function. Cytotechnology 1992;9:163-171.
40. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975;6:331-344.
41. Fusenig NE, Boukamp P. Multiple stages and genetic alterations in immortalization malignant transformation and tumor progression of human skin keratinocytes. Mol Carcinog 1998;23:144-158.
42. Toda K, Grinnell F. Activation of human keratinocyte fibronectin receptor function in relation to other ligand-receptor interactions. J Invest Dermatol 1987;88:412-417.
43. Toda K, Tuan TL, Brown PJ et al. Fibronectin receptors of human keratinocytes and their expression during cell culture. J Cell Biol 1987;105:3097-3104.
44. Leighton J. Epithelial tissue formation in matrix culture and in histophysiologic gradient culture. J Tissue Cult Methods 1992;14:201-208.
45. Lajtha LG. Stem cell concepts. Differentiation 1979;14:23-34.
46. Morrison SJ, Shah NM, Anderson DJ. Regulatory mechanisms in stem cell biology. Cell 1997;88:287-298.
47. Lavker RM, Miller S, Wilson C et al. Hair follicle stem cells: their location role in hair cycle, and involvement in skin tumor formation. J Invest Dermatol 1993;101(suppl 1):16S-26S.
48. Rongioletti F, Rebora A. Cutaneous mucinoses: microscopic criteria for diagnosis. Am J Dermatopathol 2001;23:257-267.
49. Walker AN, Morton BD. Acral mucinous syringometaplasia. A benign cutaneous lesion associated with verrucous hyperplasia. Arch Pathol Lab Med 1986;110:248-249.
50. Val-Bernal JF, Hernandez-Nieto E. Benign mucinous metaplasia of the penis. A lesion resembling extramammary Paget's disease. J Cutan Pathol 2000;27:76-79.
51. Friedman KJ, Hood AF, Farmer ER. Cutaneous squamous cell carcinoma with mucinous metaplasia. J Cutan Pathol 1988;15:176-182.
52. Potten CS, ed. Stem Cells. San Diego, CA: Academic Press Inc., 1997:1-474.
53. Sun TT, Green H. Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states. Nature 1977;269:489-493.