Expression and characterization of a recombinant cysteine proteinase of Leishmania mexicana

Biochemical Journal

Volumn 347, Issue 2, 2000, Pages 383-388

  Sanderson, Sanya J ^a   Pollock, Kevin G J ^a   Hilley, James D ^a   Meldal, Morten ^b   St Hilaire, Phaedria ^b   Juliano, Maria A ^c   Juliano, Luiz ^c   Mottram, Jeremy C ^a   Coombs, Graham H ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b CARLSBERG LABORATORY   (Denmark)

^c FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cathepsin L; Pro region processing; Recombinant enzyme

Indexed keywords

CYSTEINE PROTEINASE;

ACIDIFICATION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CELL INCLUSION; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENETIC RECOMBINATION; LEISHMANIA MEXICANA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN FOLDING; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CYSTEINE ENDOPEPTIDASES; ENZYME ACTIVATION; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; KINETICS; LEISHMANIA MEXICANA; MOLECULAR SEQUENCE DATA; PROTEIN RENATURATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

ESCHERICHIA COLI; LEISHMANIA MEXICANA; MAMMALIA; PROTOZOA;

EID: 0034656143     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3470383     Document Type: Article

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