Expression in Escherichia coli, refolding, and purification of human procathepsin K, an osteoclast-specific protease

Protein Expression and Purification

Volumn 15, Issue 2, 1999, Pages 213-220

  D'Alessio, Karla J ^a   McQueney, Michael S ^a   Brun, Kimberly A ^a   Orsini, Michael J ^b   Debouck, Christine M ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME PURIFICATION; GEL PERMEATION CHROMATOGRAPHY; PROCATHEPSIN K; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEINASE;

ESCHERICHIA COLI;

EID: 0033102214     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.1013     Document Type: Article

References (20)

1. 2. Biol. Chem. Hoppe-Seyler. 376:1995;379-384.
2. Drake F., Dodds R., James I., Connor J., Debouck C., Richardson S., Lee-Rykaczewski E., Colemen L., Rieman D., Barthlow R., Hastings G., Gowen M. Cathepsin K, but not cathepsins B, L, or S, is abundantly expressed in human osteoclasts. J. Biol. Chem. 271:1996;12511-12516.
3. Bossard M., Tomaszek T., Thompson S., Amegadzie B., Hanning C., Jones C., Kurdyla J., McNulty D., Drake F., Gowen M., Levy M. Proteolytic activity of human osteoclast cathepsin K. J. Biol. Chem. 271:1996;12517-12524.
4. Baron R. Molecular mechanisms of bone resorption by the osteoclast. Anat. Rec. 224:1989;317-324.
5. McQueney M., Amegadzie B., D'Alessio K., Hanning C., McLaughlin M., McNulty D., Carr S., Ijames C., Kurdyla J., Jones C. Autocatalytic activation of human cathepsin K. J. Biol. Chem. 272:1997;13955-13960.
6. McGrath M., Klaus J., Barnes M., Bromme D. Crystal structure of human cathepsin K complexed with a potent inhibitor. Nat. Struct. Biol. 4:1997;105-108.
7. Zhao B., Janson C., Amegadzie B., D'Alessio K., Griffin C., Hanning C., Jones C., Kurdyla J., McQueney M., Qiu X., Smith W., Abdel-Meguid S. Crystal structure of human osteoclast cathepsin K complex with E64. Nat. Struct. Biol. 4:1997;109-111.
8. Yamashita D., Smith W., Zhao B., Janson C., Tomaszek T., Bossard M., Levy M., Oh H., Carr T., Thompson S., Ijame C., Carr S., McQueney M., D'Alessio K., Amegadzie B., Hanning C., Abdel-Meguid S., DesJarlais R., Gleason J., Veber D. Structure and design of potent and selective cathepsin K inhibitors. J. Am. Chem. Soc. 119:1997;11351-11352.
9. Thompson S., Halbert S., Bossard M., Tomaszek T., Levy M., Zhao B., Smith W., Abdel-Meguid S., Janson C., D'Alessio K., McQueney M., Amegadzie B., Hanning C., DesJarlais R., Briand J., Sarkar S., Huddleston M., Ijames C., Carr S., Garnes K., Shu A., Heyes J., Bradbeer J., Zembryki D., Lee-Rykaczewski L., James I., Lark M., Drake F., Gowen M., Gleason J., Veber D. Design of potent and selective human cathepsin K inhibitors that span the active site. Proc. Natl. Acad. Sci. USA. 94:1997;14249-14254.
10. Smith S., Gottesman M. Activity and deletion analysis of recombinant human cathepsin L expressed inE. coli. J. Biol. Chem. 264:1989;20487-20495.
11. Aibe K., Yazaw H., Abe K., Teramura K., Kumegawa M., Kawashima H., Honda K. Substrate specificity of recombinant osteoclast-specific cathepsin K from rabbits. Biol. Pharm. Bull. 19:1996;1026-1031.
12. Zhao, B. Janson, C. D'Alessio, K. McQueney, M. Orsini, M. Debouck, C. Smith, W. Abdel-Meguid, S. 1997, Crystal structure of human procathepsin K at 2.6 Ångstroms resolution.
13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
14. Bachman B. J. Pedigrees of some mutant strains ofEscherichia coli. Bacteriol. Rev. 4:1972;525-557.
15. Barrett A. J., Kirschke H. Methods Enzymol. 80:1981;535-561.
16. Brinkmann U., Buchner J., Pastan I. Independent domain folding ofPseudomonas. Proc. Natl. Acad. Sci. USA. 89:1991;3075-3079.
17. Rudolph R., Lilie H. In vitro. FASEB J. 10:1996;49-56.
18. Tao K., Stearns N., Dong J., Wu Q., Sahagian G. G. The proregion of cathepsin L is required for proper folding, stability and ER exit. Arch. Biochem. Biophys. 311:1994;19-27.
19. Carmona E., Dufour E., Plouffe C., Takebe S., Mason P., Mort J., Menard R. Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases. Biochemistry. 35:1996;8149-8157.
20. Cygler M., Mort J. Proregion structure of members of the papain superfamily. Mode of inhibition of enzymatic activity. Biochimie. 79:1997;645-652.