Efficient dose-dependent and time-dependent protein transduction of pancreatic carcinoma cells in vitro and in vivo using purified VP22-EGFP fusion protein

Journal of Molecular Medicine

Volumn 81, Issue 3, 2003, Pages 205-213

  Boenicke, Lars ^a   Chu, Kang ^a   Pauls, Regina ^a   Tams, Claudia ^a   Kruse, Marie Luise ^b   Kurdow, Roland ^a   Schniewind, Bodo ^a   Böhle, Arnd ^a   Kremer, Bernd ^a   Kalthoff, Holger ^a  

^a Clin for Gen and Thoracic Surgery   (Germany)

^b Laboratory of Molecular Gastroenterology and Hepatology   (Germany)

Author keywords

Fusion protein; Nickel resin purification; Protein transduction; VP22; Xenotransplantation model

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; ICP35 PROTEIN, HUMAN HERPESVIRUS 1; NICKEL; PHOTOPROTEIN; PROTEIN VP22; RESIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ADOLESCENT; ANIMAL; APOPTOSIS; ARTICLE; CANCER THERAPY; CARCINOMA; CELL NUCLEUS; DOSE RESPONSE; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; FLUORESCENCE ANALYSIS; GENETIC TRANSDUCTION; GENETICS; HUMAN; HUMAN CELL; METABOLISM; MODEL; MOUSE; MOUSE MUTANT; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PANCREAS CARCINOMA; PANCREAS TUMOR; PATHOLOGY; PHYSIOLOGY; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN TRANSPORT; SCID MOUSE; TIME; TUMOR CELL LINE; XENOTRANSPLANTATION;

EID: 12444307569     PISSN: 09462716     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00109-003-0421-3     Document Type: Article

References (20)

1. Vile RG, Russell SJ, Lemoine NR (2000) Cancer gene therapy: hard lesson and new courses. Gene Ther 7:2-8
2. Elliott G, O'Hare P (1997) Intercellular trafficking and protein delivery by a herpes virus structural protein. Cell 88:223-233
3. Sciortino MT, Taddeo B, Poon AP, Mastino A, Roizman B (2002) Of the three tegument proteins that package mRNA in herpes simplex virions, one (VP22) transports the mRNA to uninfected cells for expression prior to viral infection. Proc Natl Acad Sci USA 99:8318-8323
4. Elliott G, O'Hare P (1999) Intercellular trafficking of VP22-GFP fusion proteins. Gene Ther 6:149-151
5. Elliott G, O'Hare P (2000) Cytoplasm-to-nucleus translocation of a herpes virus tegument protein during cell division. J Virol 74:2131-2141
6. Aints A, Guven H, Gahrton G, Smith CI, Dilber MS (2001) Mapping of Herpes simplex virus-1 VP22 functional domains for inter- and subcellular protein targeting. Gene Ther 2001 8:1051-1056
7. Phelan A, Elliott G, O'Hare P (1998) Intercellular delivery of functional p53 by the herpes virus protein VP22. Nat Biotechnol 16:440-443
8. Okabe T, Yamaguchi N, Ohsawa N (1983) Establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the exocrine pancreas. Cancer 3:662-668
9. Morgan RT, et al (1980) Human cell line (COLO 357) of metastatic pancreatic adenocarcinoma. Int J Cancer Res 25:591
10. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
11. Howard BD, Boenicke L, Schniewind B, Henne-Bruns D, Kalthoff H (2000) Transduction of human pancreatic tumor cells with vesicular stomatitis virus G-pseudotyped retroviral vectors containing a herpes simplex virus thymidine kinase mutant gene enhances bystander effects and sensitivity to ganciclovir. Cancer Gene Ther 7:927-938
12. Landis SH, Murray T, Bolden S, Wingo PA (1998) Cancer statistics. CA Cancer J Clin 48:6-29
13. Kelly DM, Benjamin IS (1995) Pancreatic carcinoma. Ann Oncol 6:19-28
14. Rosenberg L (2000) Pancreatic cancer: a review of emerging therapies. Drugs 59:1071-1089
15. Kouraklis G (2000) Gene therapy for cancer: from the laboratory to the patient. Dig Dis Sci 45:1045-1052
16. Derer W, Easwaran HP, Knopf CW, Leonhardt H, Cardoso MC (1999) Direct protein transfer to terminally differentiated muscle cells. J Mol Med 77:609-613
17. Wybranietz WA, Prinz F, Spiegel M, Schenk A, Gregor M, Lauer UM (1999) Quantification of VP22-GFP spread by direct fluorescence in 15 commonly used cell lines. J Gene Med 1:265-274
18. Fang B, Xu B, Koch P, Roth JA (1998) Intercellular trafficking of VP22-GFP fusion proteins is not observed in cultured mammalian cells. Gene Ther 5:1420-1424
19. Lundberg M, Johansson M (2002) Positively charged DNA-binding proteins cause apparent cell membrane translocation. Biochem Biophys Res Commun 2002 291:367-371
20. Cao G, Pei W, Ge H, Liang Q, Luo Y, Sharp FR, Lu A, Ran R, Graham SH, Chen J (2002) In vivo delivery of a Bcl-xL fusion protein containing the TAT protein transduction domain protects against ischemic brain injury and neuronal apoptosis. J Neurosci 22:5423-5431