Analysis of the Antigen Binding Site of Anti-Deoxycholate Monoclonal Antibody Using a Novel Affinity Labeling Reagent, Acyl Adenylate

Biochemistry

Volumn 43, Issue 7, 2004, Pages 2041-2048

  Mano, Nariyasu ^a   Nagaya, Yoko ^a   Saito, Shuntaro ^a   Kobayashi, Norihiro ^b   Goto, Junichi ^a,c  

^a TOHOKU UNIVERSITY   (Japan)

^b KOBE PHARMACEUTICAL UNIVERSITY   (Japan)

^c TOHOKU UNIVERSITY HOSPITAL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; ENZYME KINETICS; LABELING; MASS SPECTROMETRY; PH EFFECTS; SCREENING; SOLUBILITY;

ACIDIC CONDITIONS; BINDING SITES;

ANTIGENS;

ADENOSINE PHOSPHATE; DEOXYCHOLIC ACID; MONOCLONAL ANTIBODY;

AFFINITY LABELING; ANTIGEN BINDING; ARTICLE; BINDING SITE; ELECTROSPRAY MASS SPECTROMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; MOLECULAR RECOGNITION; PH; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

ADENOSINE MONOPHOSPHATE; AFFINITY LABELS; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ANTIGENS; BINDING SITES, ANTIBODY; CHOLIC ACIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROSS-LINKING REAGENTS; DEOXYCHOLIC ACID; DYNORPHINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 1242352912     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035855d     Document Type: Article

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