A mass spectrometry-based approach for probing enzyme active sites: Identification of Glu 127 in Cellulomonas fimi exoglycanase as the residue modified by N-bromoacetyl cellobiosylamine

Analytical Biochemistry

Volumn 234, Issue 2, 1996, Pages 119-125

  Tull, Dedreia ^a   Burgoyne, David L ^a   Chow, David T ^a   Withers, Stephen G ^a   Aebersold, Ruedi ^b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES;

ACID-BASE CATALYSTS; AMINO ACID DERIVATIVES; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ENZYME ACTIVE SITES; EXPERIMENTAL APPROACHES; REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STRUCTURAL CHARACTERIZATION; TANDEM MASS SPECTROMETRY;

ELECTROSPRAY IONIZATION;

AMINO ACID; GLUCAN SYNTHASE; GLUTAMIC ACID; GLYCOSIDASE; PEPSIN A; PEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELLULOMONAS; DEGRADATION; ENZYME ACTIVE SITE; MASS SPECTROMETRY; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0030020869     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0063     Document Type: Article

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