Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis

Protein Engineering

Volumn 12, Issue 5, 1999, Pages 407-415

  Miyazaki, Chie ^a   Iba, Yoshitaka ^b   Yamada, Yukio ^a   Takahashi, Haruo ^a   Sawada, Jun Ichi ^c   Kurosawa, Yoshikazu ^b  

^a Rd Management Section   (Japan)

^b FUJITA HEALTH UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Antibodies; Error prone PCR; Phage display antibody; Steroid; Structural models

Indexed keywords

CORTODOXONE; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANTIBODY COMBINING SITE; ANTIBODY SPECIFICITY; ARTICLE; BINDING AFFINITY; CLONE; COMPUTER SIMULATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; MUTAGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SURFACE PLASMON RESONANCE;

EID: 0033023075     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.5.407     Document Type: Article

References (26)

1. Arevalo, J.H., Stura, E.A., Taussig, J.J. and Wilson, I.A. (1993a) J. Mol. Biol., 231, 103-118.
2. Arevalo, J.H., Taussig, M.J. and Wilson, I.A. (1993b) Nature, 365, 859-863.
3. Berek, C. and Milstein, C. (1987) Immunol. Rev., 96, 23-41.
4. Casson, L.P. and Manser, T. (1995) J. Immunol., 155, 5647-5654.
5. Chaiken, I., Rosé, S. and Karlsson, R. (1992) Anal. Biochem., 201, 197-210.
6. Cox, J.P.L., Tomlinson, I.M. and Winter, G. (1994) Eur. J. Immunol., 24, 827-836.
7. Dauber-Osguthorpe, P., Roberts, V.A., Osguthorpe, D.J., Wolff, J., Genest, M. and Haglar, A.T. (1988) Proteins, 4, 31-47.
8. de Kruif, J., Boel, E. and Logtenberg, T. (1995) J. Mol. Biol., 248, 97-105.
9. Ding, H.Q., Karasawa, N. and Goddard, W.A. (1992) J. Chem. Phys, 97, 4309-4315.
10. Greengard, L. and Rokhlin, V.I. (1987) J. Comp. Phys., 83, 325-348.
11. Griffiths, A.D., Williams, S.C., Hartley, O., Tomlinson, I.M., Waterhouse, P., Crosby, W.L., Kontermann, E., Jones, P.T., Low, N.M., Allison, T.J., Prospero, T.D., Hoogenboom, H.R., Nissim, A., Cox, J.P.L., Harrison, J.L., Zaccolo, M., Gherardi, E. and Winter, G. (1994) EMBO J., 13, 3245-3260.
12. Hawkins, R.E., Russel, S.J. and Winter, G. (1992) J. Mol. Biol., 226, 889-896.
13. Hosoda, H., Tomura, S., Tsukamoto, R., Kobayashi, N., Sawada, J., Terao, T. and Nambara, T. (1987) Chem. Pharm. Bull., 35, 1497-1501.
14. Iba, Y., Ito, W. and Kurosawa, Y. (1997) Gene, 194, 35-46.
15. Iba, Y., Hayashi, N., Sawada, J., Titani, K. and Kurosawa, Y. (1998) Protein Engng, 11, 361-370.
16. Ichihara, Y., Matsuoka, H. and Kurosawa, Y. (1988) EMBO J., 7, 4141-4150.
17. Ito, W. and Kurosawa, Y. (1993) J. Biol. Chem., 268, 20668-20675.
18. Johnson, B., Löfas, S. and Lindquist, G. (1991) Anal. Biochem., 198, 268-277.
19. Kawasaki, K., Minoshima, S., Nakato, E., Shibuya, K., Shintani, A., Schmeits, J.L., Wang, J. and Shimizu, N. (1997) Genome Res., 7, 250-261.
20. Leung, D.W., Chen, E. and Goeddel, D.V. (1989) Technique, 1, 1-15.
21. Levitte, M. (1992) J. Mol. Biol., 226, 507-533.
22. Sawada, J., Mizusawa, S., Terao, T., Naito, M. and Kurosawa, Y. (1991) Mol. Immunol., 28, 1063-1072.
23. Schmnidt, K.E. and Lee, M.A. (1991) J. Stat. Phys., 63, 1223-1235.
24. Tomlinson, I.M., Walter, G., Marks, J.D., Llewelyn, M.B. and Winter, G. (1992) J. Mol. Biol., 227, 776-798.
25. Tonegawa, S. (1983) Nature, 302, 575-581.
26. Vaughan, T.J., Williams, A.J., Pritchard, K., Osbourn, J.K., Pope, A.R., Earnshaw, J.C., McCafferty, J., Hodits, R.A., Wilton, J. and Johnson, K.S. (1996) Nature Biotech., 14, 309-314.