Chimeragenesis of the Fatty Acid Binding Site of Cytochrome P450BM3. Replacement of Residues 73-84 with the Homologous Residues from the Insect Cytochrome P450 CYP4C7

Biochemistry

Volumn 43, Issue 7, 2004, Pages 1771-1780

  Murataliev, Marat B ^a   Trinh, Long N ^a   Moser, Lani V ^a   Bates, Robert B ^a   Feyereisen, René ^b   Walker, F Ann ^a  

^a University of Arizona   (United States)

^b CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYME KINETICS; ENZYMES; FATTY ACIDS; HYDROXYLATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OXIDATION; PURIFICATION;

BINDING SITES; MONOOXYGENATION;

BIOCHEMISTRY;

CYTOCHROME P450 BM3; CYTOCHROME P450 ISOENZYME; FATTY ACID; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; INSECT; LIPOGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LIPID INTERACTION; PROTON NUCLEAR MAGNETIC RESONANCE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BACTERIAL PROTEINS; BINDING SITES; CARRIER PROTEINS; CATALYSIS; COCKROACHES; CYTOCHROME P-450 ENZYME SYSTEM; FARNESOL; FATTY ACID-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HYDROXYLATION; INSECT PROTEINS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PALMITIC ACID; PEPTIDE FRAGMENTS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

INSECTA;

EID: 1242330318     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035674b     Document Type: Article

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