Crystal structures of the catalytic domain of HIV-1 integrase free and complexed with its metal cofactor: High level of similarity of the active site with other viral integrases

Journal of Molecular Biology

Volumn 282, Issue 2, 1998, Pages 359-368

  Maignan, Sébastien ^a   Guilloteau, Jean Pierre ^a   Zhou Liu, Qing ^a   Clément Mella, Christine ^a   Mikol, Vincent ^a  

^a NONE   (France)

Author keywords

Cacodylate; HIV; Integrase; Protein crystallization; X ray crystallography

Indexed keywords

ASPARTIC ACID; CACODYLIC ACID; INTEGRASE; MAGNESIUM; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; HUMAN IMMUNODEFICIENCY VIRUS 1; PRIORITY JOURNAL; VIRUS REPLICATION;

EID: 0032544311     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.2002     Document Type: Article

References (16)

1. Andrake, M. D. & Skalka, A. M. (1996). Retroviral integrase, putting the pieces together. J. Biol. Chem. 271, 19633-19636.
2. Ariyoshi, M., Vassylyev, D. G., Iwasaki, H., Nakamura, H., Shinagawa, H. & Morikawa, K. (1994). Atomic structure of the RuvC resolvase: a Holliday junction specific endinuclease from E. coli. Cell, 78, 1063-1072.
3. Brünger, A. T. (1992). X-PLOR Version 3.1: a system for X-ray crystallography and NMR, Yale University Press, New Haven.
4. Bujacz, G., Jaskolski, M., Alexandrakos, J., Wlodawer, A., Merkel, G., Katz, R. A. & Skalka, A. M. (1995). High resolution structure of the catalytic domain of avian sarcoma virus integrase. J. Mol. Biol. 253, 333-346.
5. Bujacz, G., Jaskolski, M., Alexandrakos, J., Wlodawer, A., Merkel, G., Katz, R. A. & Skalka, A. M. (1996a). The catalytic domain of avian sarcoma virus integrase: conformation of the active site residues in the presence of divalent cations. Structure, 4, 89-96.
6. Bujacz, G., Alexandrakos, J., Zhou-Liu, Q., Clément-Mella, C. & Wlodawer, A. (1996b). The catalytic domain of human immunodeficiency virus integrase: ordered active site in the F185H mutant. FEBS Letters, 398, 175-178.
7. Chow, S. A., Vincent, K. A., Ellison, V. & Brown, P. O. (1992). Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science, 225, 723-726.
8. Dyda, F., Hickman, A. B., Jenkins, T. M., Engelman, A., Craigie, R. & Davies, D. R. (1994). Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. Science, 266, 1981-1986.
9. Eich, E., Pertz, H., Kaloga, M., Schulz, J., Fesen, M. R., Mazumder, A. & Pommier, Y. (1996). (-)-Arctigenin as a lead structure for inhibitors of human immunodeficiency virus type-1 integrase. J. Med. Chem. 39, 86-95.
10. Engh, R. A. & Huber, R. (1991). Accurate bond and angle parameters for X-ray protein structure refinement. Acta Crystallog. sect. A, 47, 342-400.
11. Katayanagi, K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S., Ikehara, M., Matsuzaki, T. & Morikawa, K. (1990). Three-dimensional structure of ribonuclease H from E. coli. Nature, 347, 306-309.
12. Katz, R. A. & Skalka, A. M. (1994). The retroviral enzymes. Annu. Rev. Biochem. 63, 133-173.
13. Otwinowski, Z. & Minor, W. (1997). Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326.
14. Rice, P., Craigie, R. & Davies, D. R. (1996). Retroviral integrases and their cousins. Curr. Opin. Struct. Biol. 6, 76-83.
15. Thomas, M. & Brady, L. (1997). HIV integrase: a target for AIDS therapeutics. Trends Biotechnol. 15, 167-172.
16. Tsao, D. H. H. & Maki, A. H. (1991). Optically detected magnetic resonance study of the interaction of an arsenic(III) derivative of cacodylic acid with EcoRI methyl transferase. Biochemistry, 30, 4565-4572.