The drosophila U1-70K protein is required for viability, but its arginine-rich domain is dispensable

Genetics

Volumn 168, Issue 4, 2004, Pages 2059-2065

  Salz, Helen K ^a   Mancebo, Ricardo S Y ^c,d   Nagengast, Alexis A ^a   Speck, Olga ^b,e   Psotka, Mitchell ^b,f   Mount, Stephen M ^b  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

^c Howard Hughes Medical Institute   (United States)

^d APPLIED BIOSYSTEMS   (United States)

^e DUKE UNIVERSITY   (United States)

^f UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INSECT PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN; U1 70K PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; DROSOPHILA; EMBRYO DEVELOPMENT; GENE DELETION; GENETIC ANALYSIS; GENETIC TRANSFORMATION; IMMUNOPRECIPITATION; NONHUMAN; PHENOTYPIC VARIATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RNA SPLICING; SEQUENCE ANALYSIS; SURVIVAL RATE; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; ARGININE; DROSOPHILA; DROSOPHILA PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN STRUCTURE, TERTIARY; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA-BINDING PROTEINS;

ANIMALIA; ARACHNIDA; HEXAPODA; INSECTA;

EID: 11244321832     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: 10.1534/genetics.104.032532     Document Type: Article

References (29)

1. CACERES, J. F., T. MISTELI, G. R. SCREATON, D. L. SPECTOR and A. R. KRAINER, 1993 Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. EMBO J. 12: 4715-4726.
2. CAO, W., and M. A. GARCIA-BLANCO, 1998 A serine/arginine-rich domain in the human U1-70K protein is necessary and sufficient for ASF/SF2 binding. J. Biol. Chem. 273: 20629-20635.
3. CHAO, A. T., H. A. DIERICK, T. M. ADDY and A. BEJSOVEC, 2003 Mutations in eukaryotic release factors 1 and 3 act as general nonsense suppressors in Drosophila. Genetics 165: 601-612.
4. EPERON, I. C., D. C. IRELAND, R. A. SMITH, A. MAYEDA and A. R. KRAINER, 1993 Pathways for selection of 5′ splice sites by U1snRNPs and SF2/ASF. EMBO J. 12: 3607-3617.
5. EPERON, I. C., O. V. MAKAROVA, A. MAYEDA, S. H. MUNROE, J. F. CACERES et al. 2000 Selection of alternative 5′ splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF. Mol. Cell. Biol. 20: 8303-8318.
6. FLICKINGER, T. W., and H. K. SALZ, 1994 The Drosophila sex determination gene snf encodes a nuclear protein with sequence and functional similarity to the mammalian U1A snRNP protein. Genes Dev. 8: 914-925.
7. GOLOVKIN, M., and A. S. N. REDDY, 2003 Expression of U1 small nuclear ribonucleoprotein 70K antisense transcript using APET-ALA3 promoter suppresses the development of sepals and petals. Plant Physiol. 132: 1884-1891.
8. GOTTSCHALK, A., J. TANG, O. PUIG, J. SALGADO, G. NEUBAUER et al., 1998 A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA 4: 374-393.
9. HILLEREN, P. J., H.-Y. KAO and P. G. SILICIANO, 1995 The amino-terminal domain of yeast U1-70K is necessary and sufficient for function. Mol. Cell. Biol. 15: 6341-6350.
10. JAMISON, S. F., Z. PASMAN, J. WANG, C. WILL, R. LUHRMANN et al., 1995 U1 snRNP-ASF/SF2 interaction and 5′ splice site recognition: characterization of required elements. Nucleic Acids Res. 23: 3260-3267.
11. JURICA, M. S., and M. J. MOORE, 2003 Pre-mRNA splicing: awash in a sea of proteins. Mol. Cell 12: 5-14.
12. KOHTZ, J. D., S. F. JAMISON, C. L. WILL, P. Zuo, R. LUHRMANN et al., 1994 Protein-protein interactions and 5′ splice-site recognition in mammalian mRNA precursors. Nature 368: 119-124.
13. LABOURIER, E., and D. C. Rio, 2001 Purification of Drosophila snRNPs and characterization of two populations of functional U1 particles. RNA 7: 457-470.
14. MACMORRIS, M., C. BROCKER and T. BLUMENTHAL, 2003 UAP56 levels affect viability and mRNA export in Caenorhabditis elegans. RNA 9: 847-857.
15. MANCEBO, R., P. C. Lo and S. M. MOUNT, 1990 Structure and expression of the Drosophila melanogaster gene for the U1 small nuclear ribonucleoprotein particle 70K protein. Mol. Cell. Biol. 10: 2492-2502.
16. MOUNT, S. M., and H. K. SALZ, 2000 Pre-messenger RNA processing factors in the Drosophila melanogaster genome. J. Cell Biol. 150: 37-43.
17. NAGENGAST, A. A., S. M. STITZINGER, C.-H. TSENG, S. M. MOUNT and H. K. SALZ, 2003 Sex-lethal splicing autoregulation in vivo: interactions between SEX-LETHAL, the U1 snRNP and U2AF underlie male exon skipping. Development 130: 463-471.
18. PRESTON, C. R., and W. R. ENGELS, 1996 Flanking duplications and deletions associated with P-induced male recombination and gene conversion in Drosophila. Genetics 144: 1623-1638.
19. RIGAUT, G., A. SCHEVCHENKO, B. RUTZ, M. WILM, M. MANN et al., 1999 A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17: 1030-1032.
20. RUDNER, D. Z., K. S. BREGER, R. KANAAR, M. D. ADAMS and D. C. Rio, 1998a RNA binding activity of heterodimeric splicing factor U2AF: at least one RS domain is required for high-affinity binding. Mol. Cell. Biol. 18: 4004-4011.
21. RUDNER, D. Z., K. S. BREGER and D. C. Rio, 1998b Molecular genetic analysis of the heterodimeric splicing factor U2AF: the RS domain in either the large or small Drosophila subunit is dispensable in vivo. Genes Dev. 12: 1010-1021.
22. RUDNER, D. Z., R. KANAAR, K. S. BREGER and D. C. Rio, 1998c Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo. Mol. Cell. Biol. 18: 1765-1773.
23. SPRADLING, A. C., D. STERN, A. BEATON, E. J. RHEM, T. LAVERTY et al., 1999 The Berkeley Drosophila Genome Project gene disruption project: single P-element insertions mutating 25% of vital Drosophila genes. Genetics 153: 135-177.
24. STITZINGER, S., T. CONRAD, A. ZACHLIN and H. K. SALZ, 1999 Functional analysis of SNF, the Drosophila U1A/U2B″ homolog: identification of dispensable and indispensable motifs for both snRNP assembly and function. RNA 5: 1440-1450.
25. THUMMEL, C. S., and V. PIRROTTA, 1992 Technical notes: new pCasper P-element vectors. Dros. Inf. Serv. 71: 150.
26. THUMMEL, C. S., A. M. BOULET and H. D. LIPSHITZ, 1988 Vectors for P element-mediated transformation and tissue culture transformation. Gene 74: 445-456.
27. WOPPMANN, A., C. WILL, U. KORNSTADT, P. ZUO, J. MANLEY et al., 1993 Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors. Nucleic Acids Res. 21: 2815-2822.
28. Wu, J. Y., and T. MANIATIS, 1993 Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell 75: 1061-1070.
29. ZHU, J., and A. R. KRAINER, 2000 Pre-mRNA splicing in the absence of an SR protein SR domain. Genes Dev. 14: 3166-3178.