Purification of Drosophila snRNPs and characterization of two populations of functional U1 particles

RNA

Volumn 7, Issue 3, 2001, Pages 457-470

  Labourier, Emmanuel ^a   Rio, Donald C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Pre mRNA splicing; Sm proteins; SNF; U1 snRNP kinase activity; U1 70K; U1 C

Indexed keywords

PROTEIN KINASE; SMALL NUCLEAR RIBONUCLEOPROTEIN;

AMINO ACID SEQUENCE; ANTIGENICITY; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DATA BASE; DROSOPHILA; ENZYME ACTIVITY; ENZYME SPECIFICITY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN ISOLATION; PROTEIN PURIFICATION; RNA PURIFICATION; RNA SPLICING; SEQUENCE ANALYSIS; SPLICEOSOME;

AMINO ACID SEQUENCE; ANIMALS; CELL NUCLEUS; CONSERVED SEQUENCE; DROSOPHILA; GUANOSINE; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN-SERINE-THREONINE KINASES; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA CAPS; RNA SPLICING; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY;

METAZOA;

EID: 0035038080     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201001327     Document Type: Article

References (60)

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4. U2 RNA shares a structural domain with U1, U4, and U5 RNAs
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10. An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis
11. Internal protein sequence analysis: Enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes
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14. The Drosophila sex determination gene snf encodes a nuclear protein with sequence and functional similarity to the mammalian U1A snRNP protein
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31. Isolation and characterization of ribonucleoprotein complexes
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33. An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: Implications for snRNP protein function in pre-mRNA splicing
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37. RNA splicing: What has phosphorylation got to do with it?
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39. Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP
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43. U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins
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49. Split genes and RNA splicing
50. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
51. Identification and characterization of a yeast homolog of U1 snRNP-specific protein C
52. Sequence and expression of two regulated transcription units during Drosophila melanogaster development: Deb-A and Deb-B
53. Association with terminal exons in pre-mRNAs: A new role for the U1 snRNP?
54. Protein composition of mammalian spliceosomal snRNPs
55. In vitro reconstitution of mammalian U1 snRNPs active in splicing: The U1-C protein enhances the formation of early (E) spliceosomal complexes
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57. Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors
58. The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster
59. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation