Cloning and overexpression of the Exiguobacterium sp. F42 gene encoding a new short chain dehydrogenase, which catalyzes the stereoselective reduction of ethyl 3-oxo-3-(2-thienyl)propanoate to ethyl (S)-3-hydroxy-3-(2-thienyl) propanoate

Bioscience, Biotechnology and Biochemistry

Volumn 68, Issue 7, 2004, Pages 1481-1488

  Wada, Masaru ^a,c   Yoshizumi, Ayumi ^a   Furukawa, Yuki ^a   Kawabata, Hiroshi ^b   Ueda, Makoto ^b   Takagi, Hiroshi ^a   Nakamori, Shigeru ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

^b Mitsubishi Chem Grp Sci T   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Asymmetric reduction; Duloxetine; Exiguobacterium

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CLONING; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; GELS; MOLECULAR WEIGHT; PURIFICATION; SYNTHESIS (CHEMICAL);

GEL FILTRATION CHROMATOGRAPHY; INTERACTING SITES; UPTAKE CARRIERS;

GENES;

BACTERIAL DNA; OXIDOREDUCTASE; PROPIONIC ACID DERIVATIVE; THIOPHENE DERIVATIVE;

AMINO ACID SEQUENCE; ARTICLE; BACILLACEAE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BACILLACEAE; BASE SEQUENCE; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; PROPIONATES; THIOPHENES;

ESCHERICHIA COLI; EXIGUOBACTERIUM; EXIGUOBACTERIUM SP.; PROKARYOTA;

EID: 11244302527     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.68.1481     Document Type: Article

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