Subunit complementation of thymidylate synthase in Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase

Molecular and Biochemical Parasitology

Volumn 139, Issue 1, 2005, Pages 83-90

  Chanama, Manee ^a   Chitnumsub, Penchit ^b   Yuthavong, Yongyuth ^b  

^a MAHIDOL UNIVERSITY   (Thailand)

^b NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

Author keywords

Dihydrofolate reductase; Malaria; Plasmodium falciparum; Subunit complementation; Thymidylate synthase

Indexed keywords

ARGININE; CYSTEINE; DIHYDROFOLATE REDUCTASE; THYMIDYLATE SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBUNIT; HYDROGEN BOND; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTOZOON;

ANIMALS; BINDING SITES; DEOXYURACIL NUCLEOTIDES; DIMERIZATION; FOLIC ACID; GENETIC COMPLEMENTATION TEST; MULTIENZYME COMPLEXES; MUTATION, MISSENSE; PLASMODIUM FALCIPARUM; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; SUBSTRATE SPECIFICITY; TETRAHYDROFOLATE DEHYDROGENASE; THYMIDYLATE SYNTHASE;

PLASMODIUM FALCIPARUM;

EID: 11144309676     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2004.09.010     Document Type: Article

References (38)

1. C.W. Carreras, and D.V. Santi The catalytic mechanism and structure of thymidylate synthase Annu Rev Biochem 64 1995 721 762
2. P.J. Houghton, G.S. Germain, B.J. Hazelton, J.W. Pennington, and J.A. Houghton Mutants of human colon adenocarcinoma, selected for thymidylate synthase deficiency Proc Natl Acad Sci USA 86 1989 1377 1381
3. H.A. Ingraham, L. Dickey, and M. Goulian DNA fragmentation and cytotoxicity from increased cellular deoxyuridylate Biochemistry 25 1986 3225 3230
4. A. Yoshioka, S. Tanaka, and O. Hiraoka Deoxyribonucleoside triphosphate imbalance. 5-Fluorodeoxyuridine-induced DNA double strand breaks in mouse FM3A cells and the mechanism of cell death J Biol Chem 262 1987 8235 8241
5. F.M. Huennekans In search of dihydrofolate reductase Protein Sci 5 1996 1201 1208
6. H. Myllykallio, G. Lipowski, D. Leduc, J. Filee, P. Forterre, and U. Liebl An alternative flavin-dependent mechanism for thymidylate synthesis Science 297 2002 105 107
7. D. Leduc, S. Graziani, and G. Lipowski Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers Proc Natl Acad Sci USA 101 2004 7252 7257
8. S.M. Beverley, T.E. Ellenberger, and J.S. Cordingley Primary structure of the gene encoding the bifunctional dihydrofolate reductase-thymidylate synthase of Leishmania major Proc Natl Acad Sci USA 83 1986 2584 2588
9. D.J. Bzik, W.B. Li, T. Horii, and J. Inselburg Molecular cloning and sequence analysis of the Plasmodium falciparum dihydrofolate reductase-thymidylate synthase gene Proc Natl Acad Sci USA 84 1987 8360 8364
10. G.X. Chen, and J.W. Zolg Purification of the bifunctional thymidylate synthase-dihydrofolate reductase complex from the human malaria parasite Plasmodium falciparum Mol Pharmacol 32 1987 723 730
11. C.E. Garrett, J.A. Coderre, and T.D. Meek A bifunctional thymidylate synthetase-dihydrofolate reductase in protozoa Mol Biochem Parasitol 11 1984 257 265
12. P.K. Rathod, T. McErlean, and P.-C. Lee Variations in frequencies of drug resistance in Plasmodium falciparum Proc Natl Acad Sci USA 94 1997 9389 9393
13. N.J. White, and P.L. Olliaro Strategies for the prevention of antimalarial drug resistance: Rationale for combination chemotherapy for malaria Parasitol. Today 12 1996 399 401
14. Y. Yuthavong Basis for antifolate action and resistance in malaria Microbes Infect 4 2002 175 182
15. B.L. Hillcoat, and R.L. Blakely Dihydrofolate reductase of Streptococcus faecalis. I. Purification and some properties of reductase from the wild strain and from strain A J Biol Chem 241 1966 2995 3001
16. G.H. Hitching Pyrimethamine-use of an antimetabolite in the chemotherapy of malaria and other infections Clin Pharmacol Ther 1 1960 570 589
17. P.O. Olliaro, and Y. Yuthavong An overview of chemotherapeutic targets for antimalarial drug discovery Pharmco Ther 81 1999 91 110
18. I. Macreadie, H. Ginsburg, W. Sirawaraporn, and L. Tilley Antimalarial drug development and new targets Parasitol Today 16 2000 438 444
19. P.K. Rathod, and S. Reshmi Susceptibility of Plasmodium falciparum to a combination of thymidine and ICI D1694, a quinazoline antifolate directed at thymidylate synthase Antimicrob Agents Chemother 38 1994 476 480
20. M. Hekmat-Nejad, and P.K. Rathod Kinetics of Plasmodium falciparum thymidylate synthase: interactions with high-affinity metabolites of 5-fluoroorotate and D1694 Antimicrob Agents Chemother 40 1996 1628 1632
21. L. Jiang, P.C. Lee, J. White, and P.K. Rathod Potent and selective activity of a combination of thymidine and 1843U89, a folate-based thymidylate synthase inhibitor, against Plasmodium falciparum Antimicrob Agents Chemother 44 2000 1047 1050
22. S.J. Hall, P.F. Sims, and J.E. Hyde Functional expression of the dihydrofolate reductase and thymidylate synthetase activities of the human malaria parasite Plasmodium falciparum in Escherichia coli Mol Biochem Parasitol 45 1991 317 330
23. W. Sirawaraporn, R. Sirawaraporn, A.F. Cowman, Y. Yuthavong, and D.V. Santi Heterologous expression of active thymidylate synthase-dihydrofolate reductase from Plasmodium falciparum Biochemistry 29 1990 10779 10785
24. W. Sirawaraporn, P. Prapunwattana, R. Sirawaraporn, Y. Yuthavong, and D.V. Santi The dihydrofolate reductase domain of Plasmodium falciparum thymidylate synthase-dihydrofolate reductase. Gene synthesis, expression, and anti-folate-resistant mutants J Biol Chem 268 1993 21637 21644
25. S. Shallom, K. Zhang, L. Jiang, and P.K. Rathod Essential protein-protein interactions between Plasmodium falciparum thymidylate synthase and dihydrofolate reductase domains J Biol Chem 274 1999 37781 37786
26. J. Wattanarangsan, S. Chusacultanachai, J. Yuvaniyama, S. Kamchonwongpaisan, and Y. Yuthavong Effect of N-terminal truncation of Plasmodium falciparum dihydrofolate reductase on dihydrofolate reductase and thymidylate synthase activity Mol Biochem Parasitol 126 2003 97 102
27. J. Yuvaniyama, P. Chitnumsub, and S. Kamchonwongpaisan Insights into antifolate resistance from malarial DHFR-TS structures Nat Struct Biol 10 2003 357 365
28. L.W. Hardy, J.S. Finer-Moore, W.R. Montfort, M.O. Jones, D.V. Santi, and R.M. Stroud Atomic structure of thymidylate synthase: target for rational drug design Science 235 1987 448 455
29. M. Pookanjanatavip, Y. Yuthavong, P.J. Greene, and D.V. Santi Subunit complementation of thymidylate synthase Biochemistry 31 1992 10303 10309
30. P. Chitnumsub, J. Yuvaniyama, and J. Vanichtanankul Characterization, crystallization and preliminary X-ray analysis of bifunctional dihydrofolate reductase-thymidylate synthase from Plasmodium falciparum Acta Crystallogr D Biol Crystallogr 60 2004 780 783
31. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
32. B.L. Hillcoat, P.F. Nixon, and R.L. Blakley Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase Anal Biochem 21 1967 178 189
33. T.D. Meek, E.P. Garvey, and D.V. Santi Purification and characterization of the bifunctional thymidylate synthetase-dihydrofolate reductase from methotrexate-resistant Leishmania tropica Biochemistry 24 1985 678 686
34. I.H. Segal Enzyme Kinetics Behavior and Analysis of Steady-state and Rapid Equilibrium Enzyme Systems 1975 Wiley-Interscience New York
35. D. Roberts An isotopic assay for thymidylate synthetase Biochemistry 5 1966 3546 3548
36. R.H. O'Neil, R.H. Lilien, B.R. Donald, R.M. Stroud, and A.C. Anderson The crystal structure of dihydrofolate reductase-thymidylate synthase from Cryptosporidium hominis reveals a novel architecture for the bifunctional enzyme J Eukaryot Microbiol 50 Suppl. 2003 555 556
37. R.H. O'Neil, R.H. Lilien, B.R. Donald, R.M. Stroud, and A.C. Anderson Phylogenetic classification of protozoa based on the structure of the linker domain in the bifunctional enzyme, dihydrofolate reductase-thymidylate synthase J Biol Chem 278 2003 52980 52987
38. E.F. Johnson, W. Hinz, C.E. Atreya, F. Maley, and K.S. Anderson Mechanistic characterization of Toxoplasma gondii thymidylate synthase (TS-DHFR)-dihydrofolate reductase. Evidence for a TS intermediate and TS half-sites reactivity J Biol Chem 277 2002 43126 43136