Characterization of covalently inhibited extracellular lipase from Streptomyces rimusos by matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization quadrupole ion trap reflectron time-of-flight mass spectrometry: Localization of the active site serine

Journal of Mass Spectrometry

Volumn 39, Issue 12, 2004, Pages 1474-1483

  Zehl, Martin ^a   Leščić, Ivana ^b   Abramíc, Marija ^b   Rizzi, Andreas ^c   Kojić Prodić, Biserka ^b   Allmaier, Günter ^a  

^a VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^b RUDER BO KOVI INSTITUTE   (Croatia)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

3,4 Dichloroisocoumarin; Active site serine; Inhibitor modified enzyme; Matrix assisted laser desorption ionization low energy collision induced dissociation mass spectrometry; Streptomyces rimosus GDS(L) lipase

Indexed keywords

DESORPTION; DISSOCIATION; IONIZATION; MASS SPECTROMETRY; REACTION KINETICS; STOICHIOMETRY;

LIPASE INHIBITION; LIPOLYTIC ENZYME; MATRIX-ASSISTED LASER DESORPTION/IONIZATION (MALDI); SERINE RESIDUES;

AMINO ACIDS;

3,4 DICHLOROISOCOUMARIN; ACETOPHENONE DERIVATIVE; SERINE PROTEINASE INHIBITOR; TRIACYLGLYCEROL LIPASE;

CHEMICAL MODIFICATION; CONFERENCE PAPER; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ION TRAP MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; STOICHIOMETRY; STREPTOMYCES; STREPTOMYCES RIMOSUS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; LIPASE; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; SERINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STREPTOMYCES;

STREPTOMYCES; STREPTOMYCES RIMOSUS;

EID: 10844293442     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/jms.750     Document Type: Conference Paper

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