Identification of the active site nucleophile in the thermostable β- glycosidase from the archaeon Sulfolobus solfataricus expressed in Escherichia coli

Biochemistry

Volumn 36, Issue 11, 1997, Pages 3068-3075

  Febbraio, F ^a   Barone, R ^a   D'Auria, S ^a   Rossi, M ^a   Nucci, R ^a   Piccialli, G ^b   De Napoli, L ^b   Orru S ^a   Pucci, P ^a  

^a CNR   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CYANOGEN BROMIDE; EPOXIDE; GLYCOSIDASE;

ARTICLE; BINDING SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME SUBUNIT; GLYCOSYLATION; HYDROLYSIS; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; THERMOSTABILITY;

AMINO ACID SEQUENCE; BETA-GLUCOSIDASE; BINDING SITES; CLONING, MOLECULAR; ENZYME INHIBITORS; ESCHERICHIA COLI; GLUCOSIDASES; GLYCOSIDE HYDROLASES; INOSITOL; KINETICS; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SULFOLOBUS;

ARCHAEA; ESCHERICHIA COLI; SULFOLOBUS SOLFATARICUS;

EID: 0030893594     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi962496w     Document Type: Article

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