Iron handling and gene expression of the divalent metal transporter, DMT1, in the kidney of the anemic Belgrade (b) rat

Kidney International

Volumn 64, Issue 5, 2003, Pages 1755-1764

  Ferguson, Carole J ^b   Wareing, Mark ^b   Delannoy, Mathieu ^b   Fenton, Robert ^b   McLarnon, Stuart J ^b   Ashton, Nicholas ^b   Cox, Alan G ^b   McMahon, Raymond F T ^b   Garrick, Laura M ^b   Green, Roger ^b   Smith, Craig P ^b   Riccardi, Daniela ^a  

^a G38 Stopford Building   (United Kingdom)

^b NONE

Author keywords

Anemia; Divalent metal transporter DMT1; Immunohistochemistry; Inductively coupled plasma mass spectrometry; Iron; Metabolism cage studies; Northern analysis

Indexed keywords

FERROUS SULFATE; MESSENGER RNA; NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 2; POLYCLONAL ANTIBODY;

ANEMIA; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ARTIFICIAL DIET; CONTROLLED STUDY; GENE EXPRESSION; GENE MUTATION; HETEROZYGOSITY; HOMOZYGOSITY; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOREACTIVITY; IRON BLOOD LEVEL; IRON METABOLISM; IRON RESTRICTION; KIDNEY METABOLISM; MALE; MOLECULAR DYNAMICS; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; RAT; RAT STRAIN; STATISTICAL SIGNIFICANCE; URINARY EXCRETION; WESTERN BLOTTING;

EID: 10744222334     PISSN: 00852538     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1755.2003.00274.x     Document Type: Article

References (32)

1. FLEMING MD, TRENOR CC, III, SU MA, et al: Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene. Nat Genet 16:383-386, 1997
2. FLEMING MD, ROMANO MA, SU MA, et al: Nramp-2 is mutated in the anemic Belgrade (b) rat: Evidence of a role for Nramp-2 in endosomal ron transport. Proc Natl Acad Sci USA 95:1148-1153, 1998
3. VIDAL S, BELOUCHI A-M, CELLIER M, et al: Cloning and characterization of a second human NRAMP gene on chromosome 12q13. Mamm Genome 6:224-230, 1995
4. GARRICK MD, SCOTT D, WALPOLE S, et al: Iron supplementation moderates but does not cure the Belgrade anemia. Biometals 10:65-76, 1997
5. HARRISON DE: Marrow transplantation and iron therapy in mouse hereditary microcytic anemia. Blood 40:893-901, 1972
6. SLADIC-SIMIC D, ZIVKOVIC N, PAVIC D, et al: Hereditary hypochromic microcytic anemia in the laboratory rat. Genetics 53:1079-1089, 1966
7. GARRICK MD, GNIECKO K: Liu Y, et al: Transferrin and the transferrin cycle in Belgrade rat reticulocytes. J Biol Chem 268:14867-14874, 1993
8. EDWARDS JA, GARRICK LM, HOKE JE. Defective iron uptake and globin synthesis by erythroid cells in the anemia of the Belgrade laboratory rat. Blood 51:347-357, 1978
9. BOWEN BJ, MORGAN EH: Anemia of the Belgrade rat: Evidence for defective membrane transport of iron. Blood 70:38-44, 1987
10. FARCICH EA, MORGAN EH: Uptake of transferrin-bound and non-transferrin bound iron by reticulocytes from the Belgrade laboratory rat: Comparison with Wistar rat transferrin and reticulocytes. Am J Haematol 39:9-14, 1992
11. GARRICK LM, DOLAN KG, ROMANO MA, GARRICK MD: Non-transferrin bound iron uptake in Belgrade and normal rat erythroid cells. J Cell Physiol 178:349-358, 1999
12. SU MA, TRENOR III CC, FLEMING J, et al: The G185R mutation disrupts function of the iron transporter Nramp2. Blood 92:2157-2163, 1998
13. CANONNE-HERGAUX F, FLEMING MD, LEVY JE, et al: The Nramp2/ DMT1 iron transporter is induced in the duodenum of microcytic anemia mk mice but is not properly targeted to the intestinal brush border. Blood 96:3964-3970, 2000
14. CANONNE-HERGAUX F, GROS P: Expression of the iron transporter DMT1 in kidney from normal and anemic mk mice. Kidney Int 62:147-156, 2002
15. OATES PS, MORGAN EH: Defective iron uptake by the duodenum of Belgrade rats fed diets of different iron contents. Am J Physiol 270(5 Pt 1):G826-G832, 1996
16. WAREING M, FERGUSON CJ, GREEN R, et al: In vivo characterization of renal iron transport in the anaesthetized rat. J Physiol 524:581-586, 2000
17. FERGUSON CJ, WAREING M: Ward DT, et al: Cellular localization of divalent metal transporter DMT-1 in rat kidney. Am J Physiol Renal Physiol 280:F803-F814, 2001
18. OATES PS, THOMAS C, FREITAS E, et al: Gene expression of divalent metal transporter 1 and transferrin receptor in duodenum of Belgrade rats. Am J Physiol Gastrointest Liver Physiol 278:G930-G936, 2000
19. CHOMCZYNSKI P, SACCHI N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159, 1987
20. SMITH CP, LEE WS, MARTIAL S, et al: Cloning and regulation of expression of the rat kidney urea transporter (rUT2). J Clin Invest 96:1556-1563, 1995
21. GUNSHIN H, MACKENZIE B, BERGER UV, et al: Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388:482-488, 1997
22. ZAR JH: Biostatistical Analysis, Eaglewood Cliffs, NJ, Prentice Hall Inc., 1984.
23. ANDREWS NC: Iron homeostasis: Insights from genetics and animal models. Nat Rev Genet 1:208-217, 2000
24. GARRICK LM, GNIEKO K, LIU Y, et al: Iron distribution in Belgrade rat reticulocytes after inhibition of heme synthesis with succinyl-acetone. Blood 81:3414-3421, 1993
25. NUNEZ MT, ALVAREZ X, SMITH M, et al: Role of redox systems on Fe3+ uptake by transformed human intestinal epithelial (Caco-2) cells. Am J Physiol 267(6 Pt 1):C1582-C1588, 1994
26. RAJA KB, SIMPSON RJ, PETERS TJ: Investigation of a role for reduction in ferric iron uptake by mouse duodenum. Biochem Biophys Acta 10:141-146, 1992
27. CANONNE-HERGAUX F, GRUENHEID S, PONKA P, GROS P: Cellular and subcellular localization of the Nramp2 iron transporter in the intestinal brush border and regulation by dietary iron. Blood 93:4406-4417, 1999
28. VIDAL SM, PINNER E, LEPAGE P, et al: Natural resistance to intracellular infections: Nrampl encodes a membrane phosphoglycoprotein absent in macrophages from susceptible (Nramp1 D169) mouse strains. J Immunol 157:3559-3568, 1996
29. VULPE CD, KUO Y-M, MURPHY TL, et al: Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse. Nat Genet 21:195-199, 1999
30. DONOVAN A, BROWNLIE A, ZHOU Y, et al: Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. Nature 403:776-781, 2000
31. MCKIE AT, MARCIANI P, ROLFS A, et al: A novel duodenal iron-regulated transporter, IREG1, implicated in the basolateral transfer of iron to the circulation. Mol Cell 5:299-309, 2000
32. ABBOUD S, HAILE DJ: A novel mammalian iron-regulated protein involved in intracellular iron metabolism. J Biol Chem 275:19906-19912, 2000