Cloning, expression, and characterization of uracil-DNA glycosylase of Chlamydia pneumoniae in Escherichia coli

Protein Expression and Purification

Volumn 35, Issue 1, 2004, Pages 46-53

  Liu, Xipeng ^a   Liu, Jianhua ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Chlamydia pneumoniae; Expression; Purification; Uracil DNA glycosylase

Indexed keywords

CHLAMYDIA; CHLAMYDIA PNEUMONIAE; CHLAMYDOPHILA PNEUMONIAE; ESCHERICHIA COLI;

EID: 10644268702     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.12.013     Document Type: Article

References (40)

1. M.L. Dodson, R.S. Lloyd, Mechanistic comparisons among base excision repair glycosylases, Free Radic. Biol. Med. 32 (2002) 678-682.
2. U. Varshney, T. Hutcheon, J.H. van de Sande, Sequence analysis, expression, and conservation of Escherichia coli uracil DNA glycosylase and its gene (ung), J. Biol. Chem. 263 (1988) 7776-7784.
3. Y. Nakabeppu, H. Kondo, M. Sekiguchi, Cloning and characterization of the alkA gene of Escherichia coli that encodes 3-methyladenine DNA glycosylase II, J. Biol. Chem. 259 (1984) 13723-13729.
4. H. Asahara, P.M. Wistort, J.F. Bank, R.H. Bakerian, R.P. Cunningham, Purification and characterization of Escherichia coli endonuclease III from the cloned nth gene, Biochemistry 28 (1989) 4444-4449.
5. H.P. Laurence, Structure and function in the uracil-DNA glycosylase superfamily, Mutat. Res. 460 (2000) 165-181.
6. J.H. Chung, E.K. Im, H.Y. Park, J.H. Kwon, S. Lee, J. Oh, K.C. Hwang, J.H. Lee, Y. Jang, A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily, Nucleic Acids Res. 31 (2003) 2045-2055.
7. R. Argnani, F. Focher, S. Zucchini, A. Verri, G.E. Wright, S. Spadari, R. Manservigi, Herpes simplex virus type 1 (HSV-1) uracil-DNA glycosylase: functional expression in Escherichia coli, biochemical characterization, and selective inhibition by 6-(p-n-octylanilino) uracil, Virology 211 (1995) 307-311.
8. K.J. Percival, M.B. Klein, P.M. Burgers, Molecular cloning and primary structure of the uracil-DNA glycosylase gene from Saccharomyces cerevisiae, J. Biol. Chem. 264 (1989) 2593-2598.
9. H. Nilsen, M. Otterlei, T. Haug, K. Solum, T.A. Nagelhus, F. Skorpen, H.E. Krokan, Nuclear and mitochondrial uracil-DNA glycosylases are generated by alternative splicing and transcription from different positions in the UNG gene, Nucleic Acids Res. 25 (1997) 750-755.
10. P. Gallinari, J. Jiricny, A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase, Nature 383 (1996) 735-738.
11. P. Neddermann, P. Gallinari, T. Lettieri, D. Schmid, O. Truong, J.J. Hsuan, K. Wiebauer, J. Jiricny, Cloning and expression of human G/T mismatch-specific thymine-DNA glycosylase, J. Biol. Chem. 271 (1996) 12767-12774.
12. K.A. Haushalter, P.T. Stukenberg, M.W. Kirschner, G.L. Verdine, Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors, Curr. Biol. 9 (1999) 174-185.
13. A.A. Sartori, P. Schär, S. Fitz-Gibbon, J.H. Miller, J. Jiricny, Biochemical characterization of uracil processing activities in the hyperthermophilic archaeon Pyrobaculum aerophilum, J. Biol. Chem. 276 (2001) 29979-29986.
14. A.A. Sartori, S. Fitz-Gibbon, H. Yang, J.H. Miller, J. Jiricny, A novel uracil-DNA glycosylase with broad substrate specificity and an unusual active site, EMBO J. 21 (2002) 3182-3191.
15. H. Yang, S. Fitz-Gibbon, E.M. Marcotte, J.H. Tai, E.C. Hyman, J.H. Miller, Characterization of a thermostable DNA glycosylase specific for U/G and T/G mismatches from the hyperthermophilic archaeon Pyrobaculum aerophilum, J. Bacteriol. 182 (2000) 1272-1279.
16. G. Xiao, M. Tordava, J. Jagadeesh, A.C. Droha, J.T. Stivers, G. L. Gilliland, Crystal structure of Escherichia coli uracil DNA glycosylase and its complexes with uracil and glycerol: structure and glycosylase mechanism revisited, Proteins 35 (1999) 13-24.
17. R.M. Werner, Y.L. Jiang, R.G. Gordley, G.J. Jagadeesh, J.E. Ladner, G. Xiao, M. Tordova, G.L. Gilliland, J.T. Stivers, Stressing-out DNA? The contribution of serine-phosphodiester interactions in catalysis by uracil DNA glycosylase, Biochemistry 39 (2000) 12585-12594.
18. P. Handa, S. Roy, U. Varshney, The role of leucine 191 of Escherichia coli uracil DNA glycosylase in the formation of a highly stable complex with the substrate mimic, Ugi, and in uracil excision from the synthetic substrates, J. Biol. Chem. 276 (2001) 17324-17331.
19. P. Handa, N. Acharya, U. Varshney, Effects of mutations at tyrosine 66 and asparagine 123 in the active site pocket of Escherichia coli uracil DNA glycosylase on uracil excision from synthetic DNA oligomers: evidence for the occurrence of long-range interactions between the enzyme and substrate, Nucleic Acids Res. 30 (2002) 3086-3095.
20. C.D. Mol, A.S. Arvai, G. Slupphaug, B. Kavli, I. Alseth, H.E. Krokan, J.A. Tainer, Crystal structure and mutational analysis of human uracil-DNA glycosylase: structural basis for specificity and catalysis, Cell 80 (1995) 869-878.
21. G. Slupphaug, C.D. Mol, B. Kavli, A.S. Arvai, H.E. Krokan, J.A. Tainer, A nucleotide-flipping mechanism from the structure of human uracil-DNA glycosylase bound to DNA, Nature 384 (1996) 87-92.
22. R. Savva, K. McAuley-Hecht, T. Brown, L. Pearl, The structural basis of specific base-excision repair by uracil-DNA glycosylase, Nature 373 (1995) 487-493.
23. T.A. Nagelhus, T. Haug, K.K. Singh, K.F. Keshav, F. Skorpen, M. Otterlei, S. Bharati, T. Lindmo, S. Benichou, R. Benarous, H.E. Krokan, A sequence in the N-terminal region of human uracil-DNA glycosylase with homology to XPA interacts with the C-terminal part of the 34-kDa subunit of replication protein A, J. Biol. Chem. 272 (1997) 6561-6566.
24. H. Yang, J.H. Chiang, S. Fitz-Gibbon, M. Lebel, A.A. Sartori, J. Jiricny, M.M. Slupska, J.H. Miller, Direct interaction between uracil-DNA glycosylase and a proliferating cell nuclear antigen homolog in the crenarchaeon Pyrobaculum aerophilum, J. Biol. Chem. 277 (2002) 22271-22278.
25. P. Handa, N. Acharya, U. Varshney, Chimeras between single-stranded DNA-binding proteins from Escherichia coli and Mycobacterium tuberculosis reveal that their C-terminal domains interact with uracil DNA glycosylases, J. Biol. Chem. 276 (2001) 16992-16997.
26. B. Dudley, A. Hammond, W.A. Deutsch, The presence of uracil-DNA glycosylase in insects is dependent upon developmental complexity, J. Biol. Chem. 267 (1992) 11964-11967.
27. S.J. Muller, S. Caradonna, Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase, J. Biol. Chem. 268 (1993) 1310-1319.
28. r 36,000 phosphoprotein, J. Biol. Chem. 273 (1998) 21909-21917.
29. K.E. Willetts, F. Rey, I. Agostini, J.M. Navarro, Y. Baudat, R. Vigne, J. Sire, DNA repair enzyme uracil DNA glycosylase is specifically incorporated into human immunodeficiency virus type 1 viral particles through a Vpr-independent mechanism, J. Virol. 73 (1999) 1682-1688.
30. S. Priet, J.M. Navarro, N. Gros, G. Quérat, J. Sire, Functional role of HIV-1 virion-associated uracil DNA glycosylase 2 in the correction of G:U mispairs to G:C pairs, J. Biol. Chem. 278 (2003) 4566-4571.
31. D. Buxton, Potential danger to pregnant women of Chlamydia psittaci from sheep, Vet. Rec. 118 (1986) 510-511.
32. J.T. Grayston, C.C. Kuo, L.A. Campbell, S.P. Wang, Chlamydia pneumoniae sp. nov. for Chlamydia strain TWAR, Int. J. Syst. Bacteriol. 39 (1989) 88-90.
33. U. Emre, N. Sokolovskaya, P.M. Roblin, J. Schachter, M.R. Hammerschlag, Detection of anti-Chlamydia pneumoniae IgE in children with reactive airway disease, J. Infect. Dis. 172 (1995) 265-267.
34. D.L. Hahn, T. Anttila, P. Saikku, Association of Chlamydia pneumoniae IgA antibodies with recently symptomatic asthma, Epidemiol. Infect. 117 (1996) 513-517.
35. A.L. Laurila, T. Anttila, E. Laara, A. Bloigu, J. Virtamo, D. Albanes, M. Leinonen, P. Saikku, Serological evidence of an association between Chlamydia pneumoniae infection and lung cancer, Int. J. Cancer 74 (1997) 31-34.
36. C.C. Kuo, A. Shor, L.A. Campbell, H. Fukushi, D.L. Patton, J.T. Grayston, Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries, J. Infect. Dis. 167 (1993) 841-849.
37. B.J. Balin, H.C. Gerard, E.J. Arking, D.M. Appelt, P.J. Branigan, J.T. Abrams, J.A. Whittum-Hudson, A.P. Hudson, Identification and localization of Chlamydia pneumoniae in the Alzheimer's brain, Med. Microbiol. Immunol. (Berl) 187 (1998) 23-42.
38. S. Kalman, W. Mitchell, R. Marathe, C. Lammel, J. Fan, R.W. Hyman, L. Olinger, J. Grimwood, R.W. Davis, R.S. Stephens, Comparative genomes of Chlamydia pneumoniae and C. trachomatis, Nat. Genet. 21 (1999) 385-389.
39. T.D. Read, R.C. Brunham, C. Shen, S.R. Gill, J.F. Heidelberg, O. White, E.K. Hickey, J. Peterson, T. Utterback, K. Berry, S. Bass, K. Linher, J. Weidman, H. Khouri, B. Craven, C. Bowman, R. Dodson, M. Gwinn, W. Nelson, R. DeBoy, J. Kolonay, G. McClarty, S.L. Salzberg, J. Eisen, C.M. Fraser, Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39, Nucleic Acids Res. 28 (2000) 1397-1406.
40. A.C. Drohat, J. Jagadeesh, E. Ferguson, J.T. Stivers, Role of electrophilic and general base catalysis in the mechanism of Escherichia coli uracil DNA glycosylase, Biochemistry 38 (1999) 11861-11875.