An improved function for fitting sedimentation velocity data for low- molecular-weight solutes

Biophysical Journal

Volumn 72, Issue 1, 1997, Pages 435-444

  Philo, John S ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CALCULATION; MACROMOLECULE; MOLECULAR WEIGHT; SEDIMENTATION RATE;

EID: 0031036440     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(97)78684-3     Document Type: Article

References (13)

1. Claverie, J.-M. 1975. Sedimentation of generalized systems of interacting particles. I. Solution of systems of complete Lamm equations. Biopolymers. 14:1685
2. Fujita, H., and V. J. MacCosham. 1959. Extension of sedimentation velocity theory to molecules of intermediate sizes. J. Chem. Phys. 30:291-298.
3. Fujita, H. 1975. Foundations of Ultracentrifugal Analysis. John Wiley & Sons, New York. 70.
4. Hansen, J. C., J. Lebowitz, and B. Demeler. 1994. Analytical ultracentrifugation of complex macromolecular systems. Biochemistry. 33: 13155-13163.
5. Hensley, P. 1996. Defining the structure and stability of macromolecular assemblies in solution: the re-emergence of analytical ultracentrifugation as a practical tool. Structure. 4:367-373.
6. Hensley, P., C. C. Silverman, D. E. McNulty, M. L. Doyle, D. G. Myszka, T. G. Porter, and W. F. Stafford, III. 1995. The binding of an Fab to dimeric human interleukin-5: a solution interaction analysis using the time derivative method to interpret sedimentation velocity data. Beckman Instruments Discovery Seminar, Protein Society Annual Meeting, July 1995, available on the Beckman WWW site at http:// www.beckman.com/biorsrch/sympo/dscvry/binding.htm.
7. Holladay, L. A. 1979. An approximate solution of the Lamm equation. Biophys. Chem. 10:187-190.
8. Holladay, L. A. 1980. Simultaneous rapid estimation of sedimentation coefficient and molecular weight. Biophys. Chem. 11:303-308.
9. Laue, T. M., B. D. Shah, T. M. Ridgeway, and S. L. Pelletier. 1992. Computer-aided interpretation of analytical sedimentation data for proteins. In Analytical Ultracentrifugation in Biochemistry and Polymer Science. S. E. Harding, A. J. Rowe, and J. C. Horton, editors. Royal Society of Chemistry, Cambridge. 90-125.
10. Philo, J. S. 1994. Measuring sedimentation, diffusion, and molecular weights of small molecules by direct fitting of sedimentation velocity concentration profiles. In Modern Analytical Ultracentrifugation. T. M. Schuster and T. M. Laue, editors. Birkhauser, Boston. 156-170.
11. Stafford, W. F., III. 1992. Boundary analysis in sedimentation transport experiments: a procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile. Anal. Biochem. 203:295-301.
12. Stafford, W. F., III. 1994. Boundary analysis in sedimentation velocity experiments. Methods Enzymol. 240:478-501.
13. Stafford, W. F., III. 1996. Rapid molecular-weight determination by sedimentation-velocity analysis. Biophys. J. 70:MP452.