Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum

Applied and Environmental Microbiology

Volumn 70, Issue 12, 2004, Pages 7148-7155

  Netzer, Roman ^a   Peters Wendisch, Petra ^a   Eggeling, Lothar ^a   Sahm, Hermann ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIODEGRADATION; ENZYMES; GENES; GLUCOSE; METABOLISM; METABOLITES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

COMETABOLISM; CORYNEBACTERIUM GLUTAMICUM; L-SERINE;

GROWTH KINETICS;

ALANINE; CYSTATHIONINE BETA LYASE; PYRUVIC ACID; SERINE; SERINE DEHYDRATASE;

METABOLISM; MICROBIOLOGY;

AMINO ACID METABOLISM; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; GENE DELETION; GENE EXPRESSION; GENE IDENTIFICATION; GENE OVEREXPRESSION; METC GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DEGRADATION; SDAA GENE;

BACTERIAL PROTEINS; CARBON ISOTOPES; CORYNEBACTERIUM GLUTAMICUM; CULTURE MEDIA; GENE DELETION; GLUCOSE; L-SERINE DEHYDRATASE; MAGNETIC RESONANCE SPECTROSCOPY; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; SERINE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM; UNCULTURED ACTINOMYCETE;

EID: 10444221880     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.12.7148-7155.2004     Document Type: Article

References (63)

1. Awano, N., M. Wada, A. Kohdoh, T. Oikawa, H. Takagi, and S. Nakamori. 2003. Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli. Appl. Microbiol. Biotechnol. 62:239-243.
2. Brown, E. A., R. D'Ari, and E. B. Newman. 1990. A relationship between L-serine degradation and methionine biosynthesis in Escherichia coli K12. J. Gen. Microbiol. 136:1017-1023.
3. Cocaign, M., C. Monnet, and N. D. Lindley. 1993. Batch kinetics of Coryne-bacterium glutamicum during growth on various substrates: use of substrate mixtures to localize metabolic bottlenecks. Appl. Microbiol. Biotechnol. 40:526-530.
4. Crawford, I. P., and J. Ito. 1964. Serine deamination by the B protein of Escherichia coli tryptophan synthetase. Proc. Natl. Acad. Sci. USA 51:390-397.
5. Eggeling, L., and H. Sahm. 1999. L-Glutamate and L-lysine: traditional products with impetuous developments. Appl. Microbiol. Biotechnol. 52:146-153.
6. Eikmanns, B. J., N. Thum-Schmitz, L. Eggeling, K. Lüdtke, and H. Sahm. 1994. Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase. Microbiology 140:1817-1828.
7. Fariás, M. E., A. M. Strasser de Saad, A. A. Pesce de Ruiz Holgado, and G. Oliver. 1991. Purification and properties of L-serine dehydratase from Lactobacillus fermentum ATCC 14931. Curr. Microbiol. 22:205-211.
8. Flavin, M., and C. Slaughter. 1964. Cystathionine cleavage enzymes of Neurospora. J. Biol. Chem. 239:2212-2219.
9. Flint, D. H., and R. M. Allen. 1996. Iron-sulfur proteins with nonredox functions. Chem. Rev. 96:2315-2334.
10. Girbal, L., D. Hilaire, S. Leduc, L. Delery, J. L. Rols, and N. D. Lindley. 2000. Reductive cleavage of demeton-S-methyl by Corynebacterium glutamicum in cometabolism on more readily metabolizable substrates. Appl. Environ. Microbiol. 66:1202-1204.
11. Grabowski, R., A. E. Hofmeister, and W. Buckel. 1993. Bacterial L-serine dehydratases: a new family of enzymes containing iron-sulfur clusters. Trends Biochem. Sci. 18:297-300.
12. Grabowski, R., and W. Buckel. 1991. Purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent L-serine dehydratase from Peptostreptococcus asaccharolyticus. Eur. J. Biochem. 199:89-94.
13. Grant, S. G. N., J. Jessee, F. R. Bloom, and D. Hanahan. 1990. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. USA 87:4645-4649.
14. Hanahan, D. 1985. Techniques for transformation of Escherichia coli, p. 109-136. In D. M. Glover (ed.), DNA cloning, vol. 1. IRL Press, Oxford, United Kingdom.
15. Heßlinger, C., S. A. Fairhurst, and G. Sawers. 1998. Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. Mol. Microbiol. 27:477-492.
16. Hofmeister, A. E., R. Grabowski, D. Linder, and W. Buckel. 1993. L-Serine and L-threonine dehydratase from Clostridium propionicum. Two enzymes with different prosthetic groups. Eur. J. Biochem. 215:341-349.
17. Hofmeister, A. E., S. Textor, and W. Buckel. 1997. Cloning and expression of the two genes coding for L-serine dehydratase from Peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both L-serine dehydratases from Escherichia coli. J. Bacteriol. 179:4937-4941.
18. Ishige, T., M. Krause, M. Bott, V. F. Wendisch, and H. Sahm. 2003. The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses. J. Bacteriol. 185:4519-4529.
19. Jakoby, M., C. E. Ngouoto-Nkili, and A. Burkovski. 1999. Construction and application of new Corynebacterium glutamicum vectors. Biotechnol. Techniques 13:437-441.
20. Jakoby, M., M. Tesch, H. Sahm, R. Krämer, and A. Burkovski. 1997. Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I. FEMS Microbiol. Lett. 154:81-88.
21. Janke, D., and W. Fritsche. 1985. Nature and significance of microbial cometabolism of xenobiotics. J. Basic Microbiol. 25:603-619.
22. Keen, R. E., C. H. Nissenson, and J. R. Barrio. 1993. Analysis of femtomole concentrations of α-ketoisocaproic acid in brain tissue by precolumn fluorescence derivatization with 4.5-dimethoxy-1,2-diaminobenzene. Anal. Biochem. 213:23-28.
23. Keilhauer, C., L. Eggeling, and H. Sahm. 1993. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 175:5595-5603.
24. Kim, J. W., H. J. Kim, Y. Kim, M. S. Lee, and H. S. Lee. 2001. Properties of the Corynebacterium glutamicum metC gene encoding cystathionine β-lyase. Mol. Cells 11:220-225.
25. Krämer, R., C. Lambert, C. Hoischen, and H. Ebbighausen. 1990. Uptake of glutamate in Corynebacterium glutamicum. 1. Kinetic properties and regulation by internal pH and potassium. Eur. J. Biochem. 194:929-935.
26. Lange, C., D. Rittraann, V. F. Wendisch, M. Bott, and H. Sahm. 2003, Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine. Appl. Environ. Microbiol. 69:2521-2532.
27. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.
28. Marx, A., A. A. deGraaf, W. Wiechert, L. Eggeling, and H. Sahm. 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 49:111-129.
29. Marx, A., K. Striegel, A. A. deGraaf, H. Sahm, and L. Eggeling. 1997. Response of the central metabolism of Corynebacterium glutamicum to different flux burdens. Biotechnol. Bioeng. 56:168-180.
30. McFall, E., and E. B. Newman. 1996. Amino acids as carbon sources, p. 358-379. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
31. Mendz, G. L., and S. L. Maxell. 1995. Amino acid utilization by Helicobacter pylori. Int. J. Biochem. Cell. Biol. 27:1085-1093.
32. Nagata, K., Y. Nagata, T. Sato, M. A. Fujino, K. Nakajima, and T. Tamura. 2003. L-Serine, D- and L-proline and alanine as respiratory substrates of Helicobacter pylori: correlation between in vitro and in vivo amino acid levels. Microbiology 149:2023-2030.
33. Newman, E. B., and C. Walker. 1982. L-Serine degradation in Escherichia coli K-12: a combination of L-serine, glycine, and leucine used as a source of carbon. J. Bacteriol. 151:777-782.
34. Newman, E. B., and V. Kapoor. 1980. In vitro studies on L-serine deaminase activity of Escherichia coli K12. Can. J. Biochem. 58:1292-1297.
35. 14C-labeled substrates between catabolic and anabolic pathways. J. Bacteriol. 182:1136-1143.
36. Ogawa, W., T. Kayahara, M. Tsuda, T. Mizushima, and T. Tsuchiya. 1997. Isolation and characterization of an Escherichia coli mutant lacking the major serine transporter, and cloning of a serine transporter gene. J. Biochem. 122:1241-1245.
37. Petersen, S., A. A. de Graaf, L. Eggeling, M. Mollney, W. Wiechert, and H. Sahm. 2000. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J. Biol. Chem. 275:35932-35941.
38. Peters-Wendisch, P., R. Netzer, L. Eggeling, and H. Sahm. 2002. 3-Phosphoglycerate dehydrogenase from Corynebacterium glutamicum: the C-terminal domain is not essential for activity but is required for inhibition by L-serine. Appl. Microbiol. Biotechnol. 60:437-441.
39. Peters-Wendisch, P. G., V. F. Wendisch, A. A. de Graaf, B. J. Eikmanns, and H. Sahm. 1996. C3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum. Arch. Microbiol. 165:387-396.
40. Peters-Wendisch, P. G., V. F. Wendisch, S. Paul, B. J. Eikmanns, and H. Sahm. 1997. Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum. Microbiology 143:1095-1103.
41. Pfefferle, W., B. Möckel, B. Bathe, and A. Marx. 2003. Biotechnological manufacture of lysine. Adv. Biochem. Eng. Biotechnol. 79:59-112.
42. Pizer, L. I., and M. L. Potochny. 1964. Nutritional and regulatory aspects of serine metabolism in Escherichia coli. J. Bacteriol. 88:611-619.
43. Prüß, B. M., J. M. Nelms, C. Park, and A. J. Wolfe. 1994. Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids. J. Bacteriol. 176:2143-2150.
44. Riedel, C., D. Rittmann, P. Dangel, B. Möckel, S. Petersen, H. Sahm, and B. J. Eikmanns. 2001. Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production. J. Mol. Microbiol. Biotechnol. 3:573-583.
45. Rossol, I., and A. Pühler. 1992. The Corynebacterium glutamicum aecD gene encodes a C-S lyase with α,β-elimination activity that degrades aminoethylcysteine. J. Bacteriol. 174:2968-2977.
46. Rückert, C., A. Pühler, and J. Kalinowski. 2003. Genome-wide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation. J. Biotechnol. 104:213-228.
47. Sawers, G. 1998. The anaerobic degradation of L-serine and L-threonine in enterobacteria: networks of pathways and regulatory signals. Arch. Microbiol. 171:1-5.
48. Schäfer, A., A. Tauch, W. Jäger, J. Kalinowski, G. Thierbach, and A. Pühler. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.
49. Schrumpf, B., A. Schwarzer, J. Kalinowski, A. Pühler, L. Eggeling, and H. Sahm. 1991. A functionally split pathway for lysine synthesis in Corynebacterium glutamicum. J. Bacteriol. 173:4510-4516.
50. Shao, Z., R. T. Lin, and E. B. Newman. 1994. Sequencing and characterization of the sdaC gene and identification of the sdaCB operon in Escherichia coli K12. Eur. J. Biochem. 222:901-907.
51. Simic, P., H. Sahm, and L. Eggeling. 2001. L-Threonine export: use of peptides to identify a new translocator from Corynebacterium glutamicum. J. Bacteriol. 183:5317-5324.
52. Stauffer, G. V. 1996. Biosynthesis of serine, glycine, and one-carbon units, p. 506-513. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella; cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
53. Su, H., J. Moniakis, and E. B. Newman. 1993. Use of gene fusions of the structural gene sdaA to purify L-serine deaminase 1 from Escherichia coli K-12. Eur. J. Biochem. 211:521-527.
54. 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 232:433-448.
55. Umbarger, H. E. 1996. Biosynthesis of the branched-chain amino acids, p. 442-457. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
56. van der Rest, M. E., C. Lange, and D. Molenaar. 1999. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 52:541-545.
57. Velayudhan, J., M. A. Jones, P. A. Barrow, and D. J. Kelly. 2004. L-Serine catabolism via an oxygen-labile L-serine dehydratase is essential for colonization of the avian gut by Campylobacter jejuni. Infect. Immun. 72:260-268.
58. Vining, L. C., and B. Magasanik. 1981. Serine utilization by Klebsiella aero-genes. J. Bacteriol. 146:647-655.
59. Wada, M., N. Awano, K. Haisa, H. Takagi, and S. Nakamori. 2002. Purification, characterization and identification of cysteine desulfhydrase of Corynebacterium glutamicum, and its relationship to cysteine production. FEMS Microbiol. Lett. 217:103-107.
60. Wendisch, V. F. 2003. Genome-wide expression analysis in Corynebacterium glutamicum using DNA microarrays. J. Biotechnol. 104:273-285.
61. Wendisch, V. F., A. A. de Graaf, H. Sahm, and B. J. Eikmanns. 2000. Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose. J. Bacteriol. 182:3088-3096.
62. Xu, Y., and R. H. Abeles. 1993. Inhibition of tryptophan synthase by (1-fluorovinyl)glycine. Biochemistry 32:806-811.
63. Zinser, E. R., and R. Kolter. 1999. Mutations enhancing amino acid catabolism confer a growth advantage in stationary phase. J. Bacteriol. 181:5800-5807.